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本刊讯2012年度全国卫生专业技术资格考试安排已经确定,报名工作于2011年12月20日全面启动。根据人力资源和社会保障部、卫生部办公厅近日下发的《关于2012年度卫生专业技术资格考试工作有关问题 相似文献
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Objective To explore the effect of erythropoietin (EPO) on angiotensin Ⅱ (Ang Ⅱ) induced neonatal rat cardiomyocyte hypertrophy and the association with PI3K/Akt-eNOS signaling pathway. Methods Cardiomyocytes were isolated from new-born Sprague-Dawley rats and stimulated by Ang Ⅱ in vitro. The cell surface area and mRNA expression of atrial natriuretic factor (ANF) of cardiomyocytes were determined in the presence and absence of various concentrations of EPO, phosphatidylinositol 3'-kinase (PI3K) inhibitor LY294002 and nitric oxide synthase (NOS) inhibitor L-NAME. Intracellular signal molecules, such as Akt, phosphorylated Akt, eNOS and phosphorylated eNOS protein expressions were detected by western blot. Nitric oxide (NO) level in the supernatant of cultured cardiomyocytes was assayed by NO assay kit. Results EPO (20 U/ml) significantly inhibited Ang Ⅱ induced cardiomyocyte hypertrophy as shown by decreased cell surface area and ANF mRNA expression (all P <0.05). EPO also activated Akt and enhanced the expression of eNOS and its phosphorylation (all P < 0.05), increased the NO production (P <0.01). These effects could be partially abolished by cotreatment with LY294002 or L-NAME (all P < 0.05). Conclusion EPO attenuates Ang Ⅱ induced cardiomyocytes hypertrophy via activating PI3K-Akt-eNOS pathway and promoting NO production. 相似文献
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Objective To explore the effect of erythropoietin (EPO) on angiotensin Ⅱ (Ang Ⅱ) induced neonatal rat cardiomyocyte hypertrophy and the association with PI3K/Akt-eNOS signaling pathway. Methods Cardiomyocytes were isolated from new-born Sprague-Dawley rats and stimulated by Ang Ⅱ in vitro. The cell surface area and mRNA expression of atrial natriuretic factor (ANF) of cardiomyocytes were determined in the presence and absence of various concentrations of EPO, phosphatidylinositol 3'-kinase (PI3K) inhibitor LY294002 and nitric oxide synthase (NOS) inhibitor L-NAME. Intracellular signal molecules, such as Akt, phosphorylated Akt, eNOS and phosphorylated eNOS protein expressions were detected by western blot. Nitric oxide (NO) level in the supernatant of cultured cardiomyocytes was assayed by NO assay kit. Results EPO (20 U/ml) significantly inhibited Ang Ⅱ induced cardiomyocyte hypertrophy as shown by decreased cell surface area and ANF mRNA expression (all P <0.05). EPO also activated Akt and enhanced the expression of eNOS and its phosphorylation (all P < 0.05), increased the NO production (P <0.01). These effects could be partially abolished by cotreatment with LY294002 or L-NAME (all P < 0.05). Conclusion EPO attenuates Ang Ⅱ induced cardiomyocytes hypertrophy via activating PI3K-Akt-eNOS pathway and promoting NO production. 相似文献
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目的观察西洛他唑对兔动脉粥样硬化斑块组织的影响,进一步探讨西洛他唑抗动脉粥样硬化作用及其可能机制。方法将30只新西兰雄性白兔随机分为正常对照组、高脂饮食组和西洛他唑组。酶法检测血脂,免疫沉淀法测定血清C反应蛋白水平,免疫组织化学检测基质金属蛋白酶9和核因子κB的表达,病理形态学分析各组主动脉内膜厚度和斑块面积,逆转录聚合酶链反应检测血管组织单核细胞趋化蛋白1mRNA的表达。结果实验第12周末,西洛他唑组总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平低于高脂饮食组,但高于正常对照组(P均<0.01);西洛他唑组主动脉内膜厚度和斑块面积低于高脂饮食组,但大于正常对照组(P均<0.01);西洛他唑组核因子κB、单核细胞趋化蛋白1和基质金属蛋白酶9的表达弱于高脂饮食组,但强于正常对照组(P均<0.01)。结论西洛他唑有抗动脉粥样硬化作用,其作用机制可能与下调核因子κB、单核细胞趋化蛋白1及基质金属蛋白酶9的表达有关。 相似文献
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Objective To explore the effect of erythropoietin (EPO) on angiotensin Ⅱ (Ang Ⅱ) induced neonatal rat cardiomyocyte hypertrophy and the association with PI3K/Akt-eNOS signaling pathway. Methods Cardiomyocytes were isolated from new-born Sprague-Dawley rats and stimulated by Ang Ⅱ in vitro. The cell surface area and mRNA expression of atrial natriuretic factor (ANF) of cardiomyocytes were determined in the presence and absence of various concentrations of EPO, phosphatidylinositol 3'-kinase (PI3K) inhibitor LY294002 and nitric oxide synthase (NOS) inhibitor L-NAME. Intracellular signal molecules, such as Akt, phosphorylated Akt, eNOS and phosphorylated eNOS protein expressions were detected by western blot. Nitric oxide (NO) level in the supernatant of cultured cardiomyocytes was assayed by NO assay kit. Results EPO (20 U/ml) significantly inhibited Ang Ⅱ induced cardiomyocyte hypertrophy as shown by decreased cell surface area and ANF mRNA expression (all P <0.05). EPO also activated Akt and enhanced the expression of eNOS and its phosphorylation (all P < 0.05), increased the NO production (P <0.01). These effects could be partially abolished by cotreatment with LY294002 or L-NAME (all P < 0.05). Conclusion EPO attenuates Ang Ⅱ induced cardiomyocytes hypertrophy via activating PI3K-Akt-eNOS pathway and promoting NO production. 相似文献
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卢振华 《国际检验医学杂志》2011,32(2):207+209
目的分析HPV-DNA、液基薄层细胞学检查(TCT)在宫颈癌检测中的相互作用。方法对从2009年9月到2010年4月检测的537例标本进行HPV-DNA与TCT联合检测。结果 2009年9月至2010年4月检测的537例标本中HPV阳性标本74例,其中TCT异常17例,异常率为23.0%;HPV阴性标本463例,其中TCT异常58例,异常率为12.5%。结论 HPV感染者中TCT异常者较多,感染者需重视TCT检测结果,定期检查以防宫颈病变恶化。 相似文献
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目的:探讨临时起搏器在非心脏手术中常合并有心脏起搏及传导系统的功能障碍应用的必要性。方法:对60例合并缓慢型心律失常的外科患者在手术前1天或术前4h植入心脏临时起搏器,术中和术后行心电图监测记录,观察起搏器的工作状态。结果:60例中起搏器起搏有71.67%,其中严重窦性心动过缓、Ⅱ度AVB、房颤伴长间歇(>2s)和双束支阻滞的患者临时起搏器工作状态多,患者合并心肌梗死和心肌病时,是术中发生严重缓慢性心律失常的高危人群,具有临时起搏器植入的明显指征。结论:在非心脏手术中合并有缓慢型心律失常、Ⅱ度AVB、房颤伴R-R长间歇和双束支阻滞者在行外科手术前植入心脏临时起搏器可以为手术的顺利进行提供安全保障。 相似文献