Species dependent differences in the actions of sympathetic nerves and noradrenaline in the internal anal sphincter

Excitatory motor innervation to the internal anal sphincter (IAS) of the monkey, the rabbit and mouse were compared. Contractile responses to electrical field stimulation of nerves (EFS, atropine 1 micromol L(-1) and N(omega)-nitro-L-arginine 100 micromol L(-1) present throughout) were examined in isolated strips of IAS. In the monkey IAS, EFS caused frequency dependent (1-30 Hz) contractions which were abolished by guanethidine (10 micromol L(-1)) or phentolamine (3 micromol L(-1)). The sympathetic neurotransmitter noradrenaline (NA) also caused concentration-dependent (10 nmol L(-1)-100 micromol L(-1)) contractions which were abolished by phentolamine revealing a small relaxation that was abolished by propranolol (3 micromol L(-1)). In contrast, EFS caused only relaxation of the mouse and rabbit IAS which was not affected by guanethidine. Furthermore, NA relaxed these muscles and relaxation was nearly abolished by combined addition of phentolamine and propranolol. In conclusion, the monkey IAS is functionally innervated by sympathetic nerves that contract the muscle via excitatory alpha-adrenergic receptors. In contrast, no significant motor function could be identified for sympathetic nerves in the rabbit or mouse IAS although adrenergic receptors linked to muscle inhibition are present. These data reveal species dependent differences in sympathetic motor innervation and suggest that some species are more appropriate than others as models for motor innervation to the human IAS.     

慢性乙型肝炎患者血清HBV DNA水平与肝组织病理及免疫组化水平的关系

目的探讨慢性乙型肝炎患者血清HBV DNA水平与肝组织病理及免疫组化水平的关系。方法检测76例慢性乙型肝患者血清及肝组织外周血HBsAg，如BeAg和HBV DNA水平，常规病理和肝组织内的HBsAg，HBcAg。结果外周血中HBeAg阳性的患者中有91．49％HBV DNA阳性，而在如BeAg阴性的患者中，有58．62％的患者HBV DNA阳性，只有慢性乙型肝炎轻度患者的血清HBV DNA水平与其他各组差异有显著性（P〈0．05），而其余各组之间HBV DNA的复制水平均无明显差异，中度以上的血清HBV DNA多呈高水平分布。所有样本肝组织的HBsAg均为阳性，从肝组织不同HBsAg表达强度下，HBcAg表达的趋势来看，HBcAg有随着HBV DNA量的增加而表达增强的趋势。结论患者血清HBV DNA水平的变化可以反映HBV的复制水平而不能反应肝脏损伤程度。同时肝组织内的HBsAg检测更适合于低水平HBV感染的患者。     

子宫颈鳞癌患者预后影响因素及临床意义

目的分析子宫颈鳞癌Ib-IIa期手术后患者预后影响因素,建立预后预测系统,以探讨其在术后辅助治疗中的作用。方法回顾性分析74例手术治疗的Ib-IIa期宫颈鳞癌患者的临床病理资料,对其预后的因素进行分析。结果74例患者的5年生存率为74.32%,淋巴结转移、深肌层浸润、宫旁组织浸润是影响其预后的独立危险因素(P<0.05)。结论根据预后影响因素,建立预后预测系统有助于指导术后辅助治疗。     

手术前后微量元素平衡观察及其影响因素相关性分析

用原子吸收光谱法对４２ 例患儿手术前后微量元素铜、铁、锌含量进行检测,并分析了微量元素铜、铁、锌含量与年龄、手术大小、手术时间、术中输血的相关关系,提示不同因素对小儿术后微量元素含量有一定的影响。     

成年中缅树鼩大脑BDNF、trkB、ChAT mRNA及蛋白的表达

目的 观察成年中缅树鼩大脑BDNF、trkB、ChAT mRNA及蛋白的表达。方法 利用q-PCR和western blotting方法检测成年中缅树鼩大脑海马、基底核和皮层BDNF、trkB、ChAT mRNA及蛋白的表达。结果 成年树鼩大脑BDNF mRNA在海马最高,与基底核和皮层具有显著性差异（P <0.01）;trkB mRNA在海马最低,额叶皮层最高,二者间差异显著（P <0.05）;ChAT mRNA在海马、基底核和额叶皮层的表达无显著差异(P>0.05）。成年树鼩大脑BDNF蛋白表达在基底核最高,与海马或皮层有显著性差异（P <0.01）;树鼩大脑3个部位trkB、ChAT蛋白表达无显著差异(P>0.05)。 结论 成年树鼩大脑海马、基底核和皮层ChAT mRNA表达与蛋白表达具有一致性;而BDNF、trkB mRNA表达与蛋白表达不对应。提示BDNF、trkB与ChAT可能具有不同的转录水平调控模式。