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目的优选超声靶向微泡破坏(UTMD)技术介导载基因靶向纳米泡(T-NB)爆破的条件。方法采用薄膜水化法制备纳米泡,以生物素-亲和素-生物素连接方法制备pAd-AAV-9/miRNA-1 T-NB,观察其基本理化性质。基于机械指数(0.6、0.9、1.2、1.5)、T-NB溶液浓度(10^(5)/ml、10^(6)/ml、10^(7)/ml、10^(8)/ml)及辐照时间(30、45、60、120 s)设计三因素四水平正交实验,评价不同超声辐照参数对T-NB溶液信号强度的影响。结果pAd-AAV-9/miRNA-1 T-NB溶液为乳白色混悬液,浓度(0.74±0.10)×10^(8)/ml;T-NB为大小相近的球形,平均粒径(443.97±12.84)nm,平均电位(-7.33±0.09)mV。在不同辐照条件下,T-NB溶液的信号强度不同。机械指数、T-NB浓度及辐照时间均可显著影响信号强度(P均<0.05)。影响T-NB爆破的因素依次为机械指数、T-NB溶液浓度及辐照时间,机械指数0.6、T-NB浓度10^(6)/ml、辐照时间120 s为其最优组合。结论机械指数0.6、T-NB溶液浓度10^(6)/ml、辐照时间120 s为UTMD技术载基因T-NB的最优爆破条件。 相似文献
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Wilson Mathias Jeane M. Tsutsui Bruno G. Tavares Agostina M. Fava Miguel O.D. Aguiar Bruno C. Borges Mucio T. Oliveira Alexandre Soeiro Jose C. Nicolau Henrique B. Ribeiro Hsu Po Chiang João C.N. Sbano Abdulrahman Morad Andrew Goldsweig Carlos E. Rochitte Bernardo B.C. Lopes José A.F. Ramirez Roberto Kalil Filho Thomas R. Porter 《Journal of the American College of Cardiology》2019,73(22):2832-2842
BackgroundPreclinical studies have demonstrated that high mechanical index (MI) impulses from a diagnostic ultrasound transducer during an intravenous microbubble infusion (sonothrombolysis) can restore epicardial and microvascular flow in acute ST-segment elevation myocardial infarction (STEMI).ObjectivesThis study tested the clinical effectiveness of sonothrombolysis in patients with STEMI.MethodsPatients with their first STEMI were prospectively randomized to either diagnostic ultrasound–guided high MI impulses during an intravenous Definity (Lantheus Medical Imaging, North Billerica, Massachusetts) infusion before, and following, emergent percutaneous coronary intervention (PCI), or to a control group that received PCI only (n = 50 in each group). A reference first STEMI group (n = 203) who arrived outside the randomization window was also analyzed. Angiographic recanalization before PCI, ST-segment resolution, infarct size by magnetic resonance imaging, and systolic function (LVEF) at 6 months were compared.ResultsST-segment resolution occurred in 16 (32%) high MI PCI versus 2 (4%) PCI-only patients before PCI, and angiographic recanalization was 48% in high MI/PCI versus 20% in PCI only and 21% in the reference group (p < 0.001). Infarct size was reduced (29 ± 22 g high MI/PCI vs. 40 ± 20 g PCI only; p = 0.026). LVEF was not different between groups before treatment (44 ± 11% vs. 43 ± 10%), but increased immediately after PCI in the high MI/PCI group (p = 0.03), and remained higher at 6 months (p = 0.015). Need for implantable defibrillator (LVEF ≤30%) was reduced in the high MI/PCI group (5% vs. 18% PCI only; p = 0.045).ConclusionsSonothrombolysis added to PCI improves recanalization rates and reduces infarct size, resulting in sustained improvements in systolic function after STEMI. (Therapeutic Use of Ultrasound in Acute Coronary Artery Disease; NCT02410330). 相似文献
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Contrast Ultrasound Imaging of the Aorta Does Not Affect Progression of Atherosclerosis or Cardiovascular Biomarkers in ApoE−/− Mice 下载免费PDF全文
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基因治疗正逐渐成为肝细胞癌(HCC)、尤其晚期HCC的治疗方案。足够剂量的特定微RNA(miRNA)可抑制HCC细胞生长,超声微泡介导基因和药物递送治疗策略可促使miRNA于肿瘤区域靶向释放,从而达到治疗效果。本文对超声微泡介导miRNA治疗HCC研究进展进行综述。 相似文献
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目的:探讨实时心肌超声造影(RT-MCE)技术检测糖尿病(DM)早期大鼠心肌血流灌注异常的价值。方法:24只雄性SD大鼠中随机选取12只通过腹腔注射链脲佐菌素建立DM模型(DM组),剩余12只大鼠作为对照,腹腔注射等量枸橼酸缓冲液。模型建成8周后,在静息状态下,对两组大鼠行RT-MCE检查,检测两组大鼠乳头肌水平左室壁感兴趣区的峰值声学强度(A)以及造影剂的灌注速度(β),并计算出心肌血流量(A×β)。RT-MCE检查结束后,处死大鼠取心肌组织,行CD31免疫组织化学染色检测心肌微血管密度(MVD),然后对DM组A与MVD进行相关分析。结果:与对照组比较,在静息状态下DM组的A及A&#215;β较对照组明显减低,差异具有统计学意义(P<0.01),β较对照组减低,但差异无统计学意义(P>0.05)。DM组的MVD较对照组明显减低(P<0.01)。DM组A与MVD呈线性正相关(r=0.903,P<0.01)。结论:DM早期即可发生心肌血流灌注的异常,RT-MCE在检测DM早期心肌血流灌注异常方面具有重要的应用价值。 相似文献
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Donato Mele Vittorio Smarrazzo Gianni Pedrizzetti Matteo Bertini Roberto Ferrari 《Echocardiography (Mount Kisco, N.Y.)》2019,36(10):1919-1929
In the past years, assessment of cardiac function has become possible through the analysis of intracardiac flow dynamics, performed noninvasively using phase‐contrast cardiac magnetic resonance and contrast and noncontrast ultrasound techniques. From 2013 to 2019, 9 echocardiographic investigations have considered 215 patients with cardiac resynchronization therapy (CRT) as a model for assessing flow dynamics within the left ventricle. Preliminary results have been reported about the acute hemodynamic effects of CRT and programming of the CRT device, showing the potential of an approach based on analysis of intracardiac flows. At present, there are only scarce data on the capability of intracardiac flow dynamics to predict LV remodeling after CRT and no information on clinical outcome prediction. Future investigations should be aimed at clarifying the mechanisms and impact of maladaptive intracardiac vortex dynamics on progressive LV remodeling as well as the prognostic meaning of implanted CRT device based on cardiac flow analysis. 相似文献