牙本质黏结剂增强根管倒充填材料密闭性能的实验研究

目的观察在使用MTA进行根尖倒封闭术时,牙本质粘结剂对其密闭抗微渗漏的影响.方法取40颗离体单根恒牙,分别用MTA、MTA 牙本质粘结剂进行根尖倒封闭术,用染料渗透法检测根尖微渗漏的情况.结果MTA 牙本质粘结剂组的根尖密闭效果明显优于单纯使用MTA组,其差别具有统计意义(P<0.05).结论在使用MTA进行根尖倒封闭术时,使用牙本质粘结剂可提高充填体的密闭效果.     

一种候选的转移相关基因MTA1在卵巢癌中的表达

目的　为阐明卵巢癌浸润和转移的分子机理 ,我们研究了转移相关基因MTA1在卵巢癌中的表达。方法　应用逆转录多聚酶链反应 (RT -PCR)方法 ,对 4 8例卵巢组织 ,其中 8例正常卵巢 ,2 0例卵巢癌原发灶和 2 0例相配对的淋巴结进行了分析。结果　 7例有转移的卵巢癌原发灶中 ,5例MTA1mRNA过度表达 (T /N比值 >2 ) ,占 71.4 % ,无转移的原发灶MTA1mRNA过度表达为 2 3.1% (3/ 13) ,P <0 .0 5 ;7例有转移的淋巴结中 ,6例过度表达占 85 .7% ,13例无转移的淋巴结中有 2例过度表达 (15 .4 % ) ,P <0 .0 5 ;结论　MTA1基因的过度表达与卵巢癌的淋巴结转移密切相关 ,MTA1mRNA的高表达可能是评价卵巢癌恶性程度的潜在指标。     

Complex endodontic and conservative treatment of a traumatized central incisor

ObjectivesThe functional recovery of a tooth with open-apex already endodontically treated is possible thanks to the materials, such as the MTA, able to produce an effective seal of the apex which can induce a restitutio-ad-integrum. A direct composite resin restoration may then provide a coronal seal immediate and effective in time, able to ensure a complete healing of the endodontic pathology.Materials and methodsThe hereby-clinical case describes a combined approach (endodontic and conservative) for the aesthetic-functional recovery of a severely fractured upper incisor of a child of 11 years.Results and conclusionsAt the control visit, after 12 months from the completion of the coronal restoration, a complete healing of periradicular bone tissue is observed through Rx. Clinical examination showed gingival tissue without inflammation and the probing depth is not increased, despite the presence of a cavity margin placed below the gingival margin.     

MTA与氢氧化钙直接盖髓在年轻恒前牙外伤治疗中的疗效观察

目的观察MTA与氢氧化钙直接盖髓在年轻恒前牙外伤治疗中的效果。方法选取我院40例年轻恒前牙因外伤而意外露髓患者随机分成两组,分别将MTA及氢氧化钙作为手术治疗中的盖髓剂进行治疗,并在术后6个月、1年、2年及3年进行复查,分析评价疗效。结果术后6个月、1年后复查,MTA组治疗效果与氢氧化钙组相似,差异在统计学上没有可比性(P>0.05);术后2年、3年进行复查,MTA组治疗效果明显优于氢氧化钙组,两组治疗效果有显著性差异,有统计学意义(P<0.05)。结论年轻恒前牙外伤治疗中,盖髓剂应优先选择MTA,MTA的远期治疗效果明显优于氢氧化钙。     

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??As a novel pulp-capping material with good properties??MTA is used in direct pulp capping and pulpotomy. Pulp tissue responses excellently to MTA. The quality of dentin bridge induced by MTA is better than that induced by calcium hydroxide. This article summaries the role of MTA in the formation of reparative dentin when used as a pulp-capping agent.     

Success rate of three capping materials used in pulpotomy of primary molars: A randomized clinical trial

BackgroundCarious primary molars, symptomless, or with reversible pulpitis are most frequently treated with pulpotomy to maintain arch integrity, otherwise they would be extracted. The present study was conducted to assess clinically and radiographically the success rate of three capping materials: Nanohydroxyapatite (NHA), Mineral Trioxide Aggregate (MTA), and Formocresol (FC) in pulpotomy of primary molars.MethodsA clinical trial was carried out on healthy, four to eight years old children, with 72- second primary molars indicated for pulpotomy. Molars were divided into 3 equal groups (24 teeth each) designated to NHA (group 1), MTA (group 2), and FC (group 3) as pulp medicaments. Treated teeth were finally restored with stainless steel crowns. Subjects were monitored clinically and radiographically after three, six, and twelve months. Statistical analysis was presented as intended to treat analysis. Categorical data were analyzed using Fisher’s exact test. The significance level was set at p ≤ 0.05. Statistical analysis was performed using SPSS, version 26.ResultsBy the end of the twelve months, the number and percentages of successfully treated molars for Group (1), Group (2) and Group (3) were 10 (41.7%), 19 (79.2%) and 18 (75.0%) respectively; with (NHA) group showing a significantly lower rate of success, (p = 0.019).ConclusionsMTA is still the material of choice for pulpotomy in primary molars.Trial RegistrationThis trial was registered on Clincal.Trial.gov (https://clinicaltrials.gov), on February 8, 2019 (Retrospectively registered). The protocol ID is 181053. The Identifier is NCT03833557.     

新型硅酸钙基生物材料用于根尖倒充填的封闭性能和粘接强度的研究

目的:评价和比较硅酸钙基生物材料iRoot BP Plus和MTA作为根尖倒充填材料的封闭性和粘接强度。方法:收集因正畸或牙周病新鲜拔除的单根离体牙93颗,截去牙冠后行常规根管预备、根管充填,切除根尖3mm后行根尖倒预备,随机分为3组:A组(iRoot BP Plus组)、B组(MTA组)、C组(GIC组),分别行根尖倒充填。扫描电镜下观察并比较不同倒充填材料与牙本质之间粘接界面的微观形态;薄片推出实验体视显微镜下观察各组的断裂形式;测量染料渗透深度结合图像软件分析不同的微渗漏结果。结果:扫描电镜显示:iRoot BP Plus与根管壁牙体组织之间的粘接界面结合具有好的边缘适应性。薄片推出实验发现,iRoot BP Plus和MTA两组间的粘接强度无显著性差异,均明显高于GIC组(P<0.05);三组均以混合型为主要断裂方式,GIC组内粘接型断裂形式所占比例相对最高。微渗透结果显示,iRoot BP Plus和MTA的渗透深度相比无统计学差异,均明显低于GIC组(P<0.05)。结论:iRoot BP Plus与MTA作为根尖倒充填材料都具有较好的根尖封闭性能和粘接强度,均优于传统倒充填材料GIC。     

血液环境下Biodentine与MTA的颜色稳定性研究

目的比较牙体牙髓治疗中常用的骨水泥材料Biodentine与三氧化矿物凝聚体(mineral trioxide aggregrate,MTA)在与血液接触条件下的颜色稳定性,并探讨其可能的变色原因。方法制备直径5 mm、高3 mm的Biodentine和MTA圆盘,将每种材料的24个圆盘随机分配到去离子水组和脱纤维羊血组中,浸泡1 d和7 d后进行后续检测。进行各类检测的3个时间点为:圆盘固化脱模后即刻、浸泡1 d和7 d后。检测指标如下:在同样光照环境下对圆盘进行拍照,直观比较两种材料的颜色变化;采用比色仪检测两种材料的色度;借助扫描电镜观察两种材料的表面形态;使用X射线能谱仪测量两种材料各元素含量。结果肉眼观察到与脱膜后即刻相比,在脱纤维羊血组浸泡1 d后仅MTA在明暗度上发生改变,脱纤维羊血组浸泡7 d后Biodentine与MTA颜色均加深变红,但MTA暗度明显增加。比色仪结果显示脱纤维羊血组MTA的7 d色差值ΔE(21.257±0.955)大于Biodentine的7 dΔE(5.833±0.501)(t=24.781,P<0.001);MTA材料自身变色随着与血液接触时间延长而加深,其7 dΔE与1 dΔE(6.233±0.888)相比,差异具有统计学意义(t=19.956,P<0.001);而Biodentine材料自身变色随时间延长则无统计学意义[7 dΔE与1 dΔE(6.790±0.831)相比,t=1.707,P=0.163]。扫描电镜结果表明:用脱纤维羊血浸泡7 d后,MTA表面孔隙率大于Biodentine,且MTA的晶体边缘相较Biodentine也更为圆钝。X射线能谱仪检测显示:在脱纤维羊血组浸泡7 d后MTA中氧元素含量下降、铋元素含量上升;与此同时Biodentine因阻射能力低未被检测出锆元素,但其他元素含量稳定。结论Biodentine在与血液接触条件下的颜色稳定性优于MTA,这主要与Biodentine表面孔隙率小、阻射剂成分稳定有关。     

应用牙髓切断术与牙髓摘除术治疗深龋机械性露髓乳磨牙的临床效果对比研究

目的　对比研究应用矿物三氧化物凝聚体（mineral trioxide aggragate,MTA）牙髓切断术与Vitapex牙髓摘除术治疗深龋机械性露髓乳磨牙的临床效果。方法　选取2014年6—12月于第四军医大学口腔医院就诊并符合纳入标准的深龋患儿108例,共计患牙224颗。将所有患儿随机均分为牙髓切断组（119颗患牙）和牙髓摘除组（105颗患牙）,分别采用MTA牙髓切断术和Vitapex牙髓摘除术进行治疗。术后6、12、18个月随访观察两组治疗效果,并进行治疗成功率评价。结果　随访患者共92例,共计患牙192颗,其中牙髓切断组患牙101颗,牙髓摘除组患牙91颗。术后6、12、18个月牙髓切断组治疗效果均优于牙髓摘除组;比较术后18个月两组治疗成功率发现,牙髓切断组治疗成功率（90.10%）高于牙髓摘除组（79.12%）,差异有统计学意义（χ2 = 4.50,P = 0.04）。结论　相较于Vitapex牙髓摘除术,MTA牙髓切断术治疗深龋机械性露髓乳磨牙的短期疗效更好,推荐临床医生应用。