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排序方式: 共有443条查询结果,搜索用时 26 毫秒
1.
目的:调查研究四川省盐亭县中药资源、重点品种、药用植物栽培情况等,为盐亭县中药资源的开发利用提供科学依据。方法:以全国第4次中药资源普查技术规范为指导,通过野外样地调查、栽培药材调查、市场调查、民间走访调查、标本采集与制作、普查数据整理等方法,对盐亭县现有中药资源进行普查、统计和分析。结果:本次调查总共完成了36个样地、180个样方套、1 080个样方的调查,完成重点中药材调查40种,重点中药材种质资源收集40种,调查栽培药材20种,制作药用植物标本407种。结论:盐亭县中药资源种类较为丰富,但分布较为分散,中药种植产业正处于初期阶段,栽培药材种类丰富,形成了一定规模。在此基础上,应加强盐亭县中医药产业发展规划指导,充分发掘当地中药资源,对其进行合理开发、利用和保护。  相似文献   
2.
目的 探讨了失效模型和效应分析方法(Failure mode and effects analysis, FMEA)在核酸的采集、清点、运输和接收等全过程中的应用和有效性。方法 便利抽样法,以从2020年11月至2021年4月在某院进行核酸采样的标本为主要研究对象。将2020年11月至2021年1月进行的核酸采样标本(共10704例)作为对照组,采用常规核酸采样流程;将2021年2月至2021年4月进行核酸采样标本(共10541例)作为观察组,通过经过FMEA优化后的应用信息化手段核酸采样全过程控制,对已筛查出来的高危失效模型实施有针对性的整改。结果 表明在经过FMEA优化后,观察组高危失效模型的RPN值显著下降,核酸标本丢失率均显著降低,标本扫码执行率、标本数量正确率提高(均P<0.05)。结论利用FMEA对核酸标本进行全程信息化安全管理,可以减少差错的发生,缓解医患矛盾,节约人力。  相似文献   
3.
Acute pancreatitis (AP) is an inflammatory disease with high morbidity and mortality. Dysregulation of microRNAs (miRNAs) was involved in human diseases, including AP. However, the effects of miR-92b-3p on AP process and its mechanism remain not been fully clarified. The expression levels of miR-92b-3p and tumor necrosis factor receptor-associated factor-3 (TRAF3) were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The protein levels of TRAF3, tumor necrosis factor α (TNF-α) TNF-α, interleukin-6 (IL-6), phosphorylated mitogen-activated protein kinase kinase 3 (p-MKK3), MKK3, p38 and phosphorylated p38 (p-p38) were detected by western blot. The concentration of TNF-α and IL-6 in the medium was measured using ELISA kits. The possible binding sites of miR-92b-3p and TRAF3 were predicted by TargetScan and verified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. The expression level of miR-92b-3p was decreased and TRAF3 expression was increased in AR42J cells stimulated with caerulein. Moreover, the protein levels of pro-inflammatory cytokines (TNF-α and IL-6) were markedly elevated, and the expression levels of autophagy-related markers Beclin1 as well as the ratio of LC3-II/I were obviously increased in AR42J cells treated with caerulein. In addition, overexpression of miR-92b-3p or knockdown of TRAF3 significantly suppressed the release of pro-inflammatory cytokines and autophagy in caerulein-induced AR42J cells. Furthermore, TRAF3 was a direct target of miR-92b-3p and its upregulation reversed the effects of miR-92b-3p overexpression on inflammatory response and autophagy. Besides, overexpression of miR-92b-3p inhibited the activation of the MKK3-p38 pathway by affecting TRAF3 expression. In conclusion, miR-92b-3p attenuated inflammatory response and autophagy by downregulating TRAF3 and suppressing MKK3-p38 pathway in caerulein-induced AR42J cells, providing a novel avenue for treatment of AP.  相似文献   
4.
目的:研究影响骨髓造血干细胞动员及采集的因素。方法方便选取2013年3月_2016年6月期间就诊该院的77名健康供者,经重组人粒细胞集落刺激因子(rhG-CSF﹚动员后第4天采集骨髓,分析不同健康供者的年龄和性别对骨髓造血干细胞采集的影响以及采集时外周血白细胞数与采集效果的关系。结果77名供者均成功采集,骨髓采集物单个核细胞数(BM-MNCs)为(4.3±0.43﹚×108/kg,骨髓采集物CD34+细胞数(BM-CD34+﹚为(1.84±0.30﹚×106/kg,健康供者年龄和性别差异无统计学意义,采集时外周血白细胞数不能预测骨髓采集物中CD34+细胞的数量。结论采用5~10μg/kg G-CSF动员,健康供者均可成功采集骨髓干细胞。  相似文献   
5.
目的详细分析初次全血采集量不足原因。方法对2016-2018年焦作献全血情况进行调查,甄选出初次全血采集量不足者,细致探讨造成采集量不足的的原因。结果造成初次全血采集量不足原因,主要有7项,分别为精神因素、空腹献血、坐姿不当、采血者穿刺水平较低、个人因素、止血带以及环境因素。结论根据全血采集量不足原因制定一系列处理方案,可降低初次全血采集量不足的发生几率,能够更好地救治需要输血的患者。  相似文献   
6.
7.

Objective

To identify current practice patterns for unresolved issues in the surgical and adjuvant management of endometrial cancer in Korea.

Methods

We designed and conducted a survey of all 218 active members of the Korean Gynecologic Oncology Group to try to identify how they would manage various case scenarios for endometrial cancer. Data were collected using an Internet survey database.

Results

A total of 108 members (49.5%) responded to the survey. Laparoscopy (81.6%) was the most commonly used mode of surgery in early-stage endometrial cancer. Of all the respondents, 19.8% stated that lymphadenectomy could be omitted and 21.7% recommended selective lymphadenectomy based on sentinel biopsy or frozen results for patients with presumed stage IA/grade 1 disease. On the other hand, 71.9% of respondents recommended para-aortic lymphadenectomy for patients with presumed stage IB/grade 1 disease and 86.4% recommended this treatment for presumed stage IB/grade 3 disease. The majority of respondents performed adjuvant therapy for stage IB/grade 2 (91.7%), IB/grade 3 (99.0%), and stage II (89.6%). Whole pelvic radiotherapy and vaginal brachytherapy were the most frequently used options among these patients. All respondents administered adjuvant therapy when node metastasis was found, and concurrent chemoradiotherapy (53.2%) was the most preferred option for stage IIIC1 disease.

Conclusion

There is broad variation in both the surgical and adjuvant treatment of endometrial cancer among Korean gynecologic oncologists.  相似文献   
8.
Microbial detoxification of deoxynivalenol (DON) represents a new approach to treating DON-contaminated grains. A bacterium Devosia mutans 17-2-E-8 was capable of completely transforming DON into a major product 3-epi-DON and a minor product 3-keto-DON. Evaluation of toxicities of these DON-transformation products is an important part of hazard characterization prior to commercialization of the biotransformation application. Cytotoxicities of the products were demonstrated by two assays: a MTT bioassay assessing cell viability and a BrdU assay assessing DNA synthesis. Compared with DON, the IC50 values of 3-epi-DON and 3-keto-DON were respectively 357 and 3.03 times higher in the MTT bioassay, and were respectively 1181 and 4.54 times higher in the BrdU bioassay. Toxicological effects of 14-day oral exposure of the B6C3F1 mouse to DON and 3-epi-DON were also investigated. Overall, there were no differences between the control (free of toxin) and the 25 mg/kg bw/day or 100 mg/kg bw/day 3-epi-DON treatments in body and organ weights, hematology and organ histopathology. However, in mice exposed to DON (2 mg/kg bw/day), white blood cell numbers and serum immunoglobulin levels were altered relative to controls, and lesions were observed in adrenals, thymus, stomach, spleen and colon. Taken together, in vitro and in vivo studies indicate that 3-epi-DON is substantially less toxic than DON.  相似文献   
9.
《Vaccine》2016,34(3):395-400
Brucellosis is a wide spread zoonotic disease that causes abortion and infertility in mammals and leads to debilitating, febrile illness in humans. Brucella abortus, Brucella melitensis and Brucella suis are the major pathogenic species to humans. Vaccination with live attenuated B. suis strain 2 (S2) vaccine is an essential and critical component in the control of brucellosis in China. The S2 vaccine is very effective in preventing brucellosis in goats, sheep, cattle and swine. However, there are still debates outside of China whether the S2 vaccine is able to provide protection against heterologous virulent Brucella species. We investigated the residual virulence, immunogenicity and protective efficacy of the S2 vaccine in BALB/c mice by determining bacteria persistence in spleen, serum antibody response, cellular immune response and protection against a heterologous virulent challenge. The S2 vaccine was of low virulence as there were no bacteria recovered in spleen four weeks post vaccination. The vaccinated mice developed Brucella-specific IgG in 2–3 weeks, and a burst production of IFN-γ at one week as well as a two-fold increase in TNF-α production. The S2 vaccine protected mice from a virulent challenge by B. melitensis M28, B. abortus 2308 and B. suis S1330, and the S2 vaccinated mice did not develop any clinical signs or tissue damage. Our study demonstrated that the S2 vaccine is of low virulence, stimulates good humoral and cellular immunity and protects animals against infection by heterologous, virulent Brucella species.  相似文献   
10.
《药学学报(英文版)》2020,10(8):1453-1475
Angiokinases, such as vascular endothelial-, fibroblast- and platelet-derived growth factor receptors (VEGFRs, FGFRs and PDGFRs) play crucial roles in tumor angiogenesis. Anti-angiogenesis therapy using multi-angiokinase inhibitor has achieved great success in recent years. In this study, we presented the design, synthesis, target identification, molecular mechanism, pharmacodynamics (PD) and pharmacokinetics (PK) research of a novel triple-angiokinase inhibitor WXFL-152. WXFL-152, identified from a series of 4-oxyquinoline derivatives based on a structure–activity relationship study, inhibited the proliferation of vascular endothelial cells (ECs) and pericytes by blocking the angiokinase signals VEGF/VEGFR2, FGF/FGFRs and PDGF/PDGFRβ simultaneously in vitro. Significant anticancer effects of WXFL-152 were confirmed in multiple preclinical tumor xenograft models, including a patient-derived tumor xenograft (PDX) model. Pharmacokinetic studies of WXFL-152 demonstrated high favourable bioavailability with single-dose and continuous multi-dose by oral administration in rats and beagles. In conclusion, WXFL-152, which is currently in phase Ib clinical trials, is a novel and effective triple-angiokinase inhibitor with clear PD and PK in tumor therapy.  相似文献   
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