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1.
结核分枝杆菌DNA指纹技术及其应用研究   总被引:3,自引:3,他引:9  
目的 从分子流行病学角度探讨北京及其他地区结核分枝杆菌(结核菌)的分布特征。方法 构建以IS6110为基础的结核菌DNA指纹图谱,应用MINTS软件进行处理,并用χ2检验比较不同组别结核病人临床分离菌株成簇率的差别。结果 H37Rv、BCG两个标准菌株和62例结核病人临床分离菌株IS6110DNA指纹结果与国外同类报道一致;其中70%(44/62)的临床分离菌株IS6110DNA指纹相似值在1.00~0.65之间;分组统计结果显示,男性组与女性组成簇率之间差异有显著性(P<0.05),其他各组之间差异无显著性(P>0.05)。结论 DNA指纹技术对结核菌在株水平的鉴定具有特异、灵敏等优点,可应用于结核流行病学研究。研究表明,北京及其他地区结核菌临床分离菌株多数遗传关系较近,且在基因水平上相关程度较强;结核菌在男性人群中的传播频率可能较女性更高。  相似文献
2.
多重耐药性鲍曼不动杆菌耐药基因及菌株聚类分析   总被引:2,自引:0,他引:2  
目的了解临床分离到的鲍曼不动杆菌耐药相关基因存在状况和菌株间的亲缘性。方法药敏试验用PhoenixTM100系统检测。用PCR扩增β-内酰胺酶基因、氨基糖甙类药物修饰酶基因、DNA拓扑异构酶基因等,并用DNA测序仪证实。结果247株鲍曼不动杆菌对亚胺培南和美洛培南耐药率最低,仅为3.2%、4.1%。20株多重耐药性鲍曼不动杆菌中,17株具TEM型β-内酰胺酶基因,氨基糖甙类药物修饰酶基因aac(3)-Ⅰ、aac(3)-Ⅱ、aac(3)-Ⅲ、aac(3)-Ⅳ、aac(6′)-Ⅰ、ant(2″)-Ⅰ、ant(3″)-Ⅰ基因检出率分别为80%、15%、30%、70%、15%、55%、80%。20株对环丙沙星耐药的鲍曼不动杆菌gyrA密码子均由TCA→TTA,氨基酸由Ser83→Leu,而85%菌株(17/20)的ParC氨基酸序列发生Ser80→Leu替代。聚类分析显示存在2株克隆株,其耐药表型基本一致。结论本院鲍曼不动杆菌携带多种β-内酰胺酶、氨基糖甙类修饰酶等耐药基因,喹诺酮耐药决定区基因突变是细菌耐受喹诺酮类药物重要原因。聚类分析表明,本院的鲍曼不动杆菌存在克隆传播。  相似文献
3.
用于功能磁共振成像数据处理的时间簇分析法   总被引:1,自引:1,他引:0  
作为一种新的数据处理方法,在大脑激活的位置和时间信息完全未知的情况下,时间簇分析(TCA)可以基于每个时间点达到最大值的像素的个数或灰度值得到大脑激活的时间信息.该方法具有算法容易实现,计算量小等优点,但其灵敏度低.目前对该方法的研究还停留在单层磁共振数据上,限制了其应用.  相似文献
4.
INTRODUCTION: Cancer patients experience multiple concurrent symptoms. This exploratory analysis assessed symptom burden among patients undergoing chemotherapy for breast cancer to identify distinct subgroups of patients who experience differential symptom burden and assessed whether the patient subgroups were associated with deleterious quality of life (QOL) outcomes. MATERIALS AND METHODS: Women (N = 133) with stage I and II breast cancer undergoing adjuvant chemotherapy after primary surgery were evaluated at baseline and at the end of chemotherapy using the Memorial Symptom Assessment Scale (MSAS) and the SF-36 QOL questionnaire. Post treatment MSAS symptoms were included in hierarchical cluster analysis. Two patient subgroups were identified that corresponded to a high-symptom prevalence group and a low-symptom group. RESULTS AND DISCUSSION: No marked, statistically significant differences were found between groups on demographic, symptoms, QOL, or treatment variables at baseline. Patients in the high-symptom cluster were more likely to have stage I disease (p < 0.05). The two groups of patients showed significant differences in end-of-treatment symptoms and QOL scores (p < 0.05). The high-symptom burden group was more likely to report greater symptom prevalence and poorer QOL. CONCLUSIONS: Future research needs to examine why these differences occur despite similarities in treatment and how symptom burden can be reduced for the high-symptom prevalence group.  相似文献
5.
磁共振观察运动准备与执行的脑功能分布   总被引:1,自引:0,他引:1       下载免费PDF全文
目的 探索运动指令与运动执行的脑功能区空间分布.方法 被试者为15名健康大学生,根据屏幕上出现的指令,分别进行运动准备和手指对指运动,磁共振采集数据,以最大峰值作为聚类依据,自编聚类程序,对作业活动脑区进行聚类统计处理.结果 在M1区出现大面积的运动激活,且有少量运动准备活动出现;发现SMA、PPC等辅助运动区有较大面积运动准备激活,也有少量运动执行活动的脑激活.在运动脑区,两种功能活动的空间分布清晰.结论 M1、SMA、PPC等脑区并非单一脑功能区,而是多功能混合脑区.  相似文献
6.
Is There a Biological Basis for the Clustering of Symptoms?   总被引:1,自引:0,他引:1  
OBJECTIVES: To examine symptom clusters in oncology patients; to determine if these symptom clusters might share a common biologic mechanism; and to describe potential biologic mechanisms that warrant investigation. DATA SOURCES: Synthesis of the theoretical and research papers on symptom clusters. CONCLUSION: Definitive conclusions about whether there is a biologic basis for the clustering of symptoms cannot be determined at this time. The animal model of sickness behavior holds promise as a potential biologic mechanism for clustering symptoms. IMPLICATIONS FOR NURSING PRACTICE: Until more definitive studies of symptom clusters are performed, clinicians need to monitor patients for the co-occurrence of multiple symptoms and develop appropriate management plans.  相似文献
7.
市场细分技术在武汉市无偿献血者招募中的应用   总被引:1,自引:1,他引:0  
目的了解目标献血者的构成和轮廓,以提高血站运作效率,并使目标献血者最大化地响应招募。方法借鉴社会营销中的市场细分技术,选择无偿献血知信行(KAP)、生活方式和人口统计变量作为细分变量,使用聚类分析的对武汉市居民行细分分析:向18—55周岁的武汉市居民及无偿献血者发放问卷,并对所收回的1101份有效问卷作市场(群体)细分。结果分析得到4个细分群体,依据集群中心距离判断各个细分群体的最重要特征,依次为“自我意识型”、“健康关注型”、“积极合作型”和“疏远淡漠型”。结论4个细分群体在生活方式、献血动机与行为上有明显差异;血站对各个细分群体的无偿献血宣传和营销活动应根据不同的营销目标而采取相宜的招募策略。  相似文献
8.
9.
A survey of the psychologic profile of 540 chronic headache cases, including migraine, tension, and mixed headache, has been carried out with the symptom check list (SCL)-90-R inventory. The results obtained were viewed in relation to sex, age, illness onset, and illness duration. Females showed a positive correlation of somatization with present age and with age at onset of illness and a negative correlation of interpersonal sensitivity, hostility, and paranoid ideation with present age and age at onset of illness. Men showed a negative correlation of obsessive-compulsive and paranoid scores with age and a positive correlation between onset of illness and somatization. Data were also analyzed by cluster analysis, which showed underpopulation with peculiar patterns of symptom profile. A prospective, long-term epidemiologic study could provide more conclusive results.  相似文献
10.
The role of psychological factors in the course of primary headache syndromes is still controversial. Using the Minnesota Multiphasic Personality Inventory (MMPI) we investigated the personality profiles of 434 headache patients (160 migraineurs, 95 with tension type headache, 30 with cluster headache and 149 with combination headache) in accordance with the IHS criteria. In the first three MMPI scales (hypochondria, depression, hysteria) there was a slight increase in T mean values to over 60, but still in the range of two standard deviations of the normal population. There were no statistically significant differences between the four headache groups and between patients with and without analgesic abuse. It was impossible to distinguish headache groups on the basis of their personality profiles by means of reclassification with discriminant analysis. In a cluster analysis, patients with cluster headache showed the highest number (20%) of abnormalities, but also the highest percentage (13%) of completely normal results. Our findings--a cross section analysis of personality profiles--contradict many other MMPI-based studies.  相似文献
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