排序方式: 共有62条查询结果,搜索用时 15 毫秒
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目的:摸索抑制神经干细胞(NSCs)中β-catenin表达的合适条件,为获得抑制β-catenin表达的NSCs做准备。利用抑制β-catenin表达的NSCs,探索wnt/β-catenin通路在脉冲强磁场促NSCs增殖中的作用。方法:取新生的SD大鼠双侧侧脑室下的NSCs,然后用无血清培养基培养14d。利用RNAi原理构建β-catenin shRNA慢病毒载体及阴性慢病毒载体,并转染NSCs,确定合适的感染复数(MOI)值。将NSCs分为阴性组(阴性病毒转染组)和实验组(β-catenin shRNA病毒转染组),并用4T、0.1Hz、8次的脉冲强磁场干预。在用脉冲强磁场干预后的1d、7d,采用CCK8法检测阴性组和实验组的NSCs增殖情况。结果:β-catenin shRNA序列在MOI=10时,对NSCs中β-catenin的抑制效果最好。阴性病毒组在脉冲强磁场干预后1d、7d时NSCs增殖最明显(P0.05),而实验组在干预前后NSCs增殖水平无明显变化。结论:wnt/β-catenin通路在脉冲强磁场促NSCs增殖中起作用。 相似文献
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目的分析和评价舰员疲劳状态和某舰艇舱室空气质量,找出可能导致舰员疲劳的原因,为维护和提高长航官兵的健康提供理论依据和指导意见。方法采用《疲劳自觉症状调查表》对舰员出航前后疲劳状况进行调查分析。采用Gasmet DX4035型傅立叶红外多组分气体分析仪对航行舰艇各个舱室的空气质量进行检测,并依据环境质量标准对舰艇舱室空气质量进行评价。结果 (1)经过1周的航行后,所有舰员疲劳症状明显加重,"身体"、"精神"、"神经感觉"3种疲劳因子均明显升高。(2)部分舱室空气中甲醛、SO2超标。结论舰员出海后较出海前疲劳程度明显加重;该舰某些舱室空气中部分有害气体含量超标,从而可能加重舰员的疲劳。 相似文献
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BACKGROUND: Hyperbaric oxygen (HBO) therapy can alleviate the skin flap congestion by improving the angiogenesis and increasing the oxygen content of blood in skin flaps. Although the HBO therapy ability to increase flap survival has been well described, the research on the application of HBO pretreatment in skin flap transplantation does not arouse adequate concern.
OBJECTIVE: To investigate the effect of HBO pretreatment on early-stage flap congestion in the rat model of over-length dorsal random skin flaps.
METHODS: Thirty-six SD rats were randomly divided into control group (n=12), HBO pretreatment group (n=12) and HBO treatment group (n=12). Rats in the HBO pretreatment group received 4 days of HBO therapy prior to transplantation, once a day. Rats in the HBO treatment group received 4 days of HBO therapy after transplantation. Rats in the control group were raised in the normal conditions after flap transplantation. At postoperative days 3 and 5, rats were sacrificed and the samples were collected. The inflammation of flap tissues was detected using hematoxylin-eosin staining. The expression of vascular endothelial growth factor and transforming growth factor-β was analyzed by immunohistochemistry staining. The flap survival rate was calculated at postoperative day 5.
RESULTS AND CONCLUSION: The flap survival area of the HBO pretreatment group and HBO treatment group was larger than that of the control group (P < 0.05). At postoperative days 3 and 5, the expression levels of vascular endothelial growth factor and transforming growth factor-β in the flap tissue were higher in the HBO pretreatment group and HBO treatment group than the control group (P < 0.05). However, there was no significant difference in the flap survival area and the expression of vascular endothelial growth factor and transforming growth factor-β between HBO pretreatment and HBO treatment groups (P > 0.05). HBO pretreatment can increase the expression of vascular endothelial growth factor and transforming growth factor-β and promotes angiogenesis in random pattern flaps, thereby improving skin flap survival. 相似文献
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一直以来,我国主流的医疗服务市场(医院)基本上无“市场运作”这一概念。一纸公文任命的院领导、施合般的服务方式普遍有之。大中型国有医院处于垄断性经营的地位,无须考虑生存,不愁病源匮乏,旱涝保收,风雨无忧……在“温室”中久了,不期然就滋生了一些流行“病毒”: 相似文献
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目的探讨脉冲强磁场对体外新生大鼠神经干细胞(NSCs)向神经元方向分化的作用。 方法体外分离培养新生3d内的SD大鼠脑室下区的NCSs,无血清培养14d后,随机分为实验组和对照组。实验组置于脉冲强磁场条件下用前期探索的促增殖参数(0.1Hz、4T、8次)进行干预,每次脉冲放电20ms。对照组置于相同条件下但不做干预处理。干预后第1天,采用10%胎牛血清诱导贴壁分化,显微镜下观察不同时间段细胞形态学变化;贴壁分化后第7天,通过免疫荧光染色、蛋白免疫印迹法(Western Blot)及实时定量聚合酶链反应技术(RT-PCR)检测NCSs分化后第7天神经元βⅢ微管蛋白(TUJ1)及星形胶质细胞相关特异性标志神经胶质纤维酸性蛋白(GFAP)的表达。 结果干预后,免疫荧光染色示实验组TUJ1阳性细胞数为(33.4±5.1)%,较对照组[(26.5±7.0)%]明显增多(P<0.05),实验组的GFAP阳性细胞率[(23.9±5.0)%]较对照组[(36.2±2.2)%]明显减少 (P<0.05)。RT-PCR检测显示,实验组TUJ1的mRNA表达量是对照组的(1.682±0.086)倍,GFAP的mRNA相对表达量是对照组的(0.590±0.157)倍,且组间差异有统计学意义(P<0.05)。Western Blot检测结果与RT-PCR检测一致,实验组TUJ1蛋白表达较对照组增多,GFAP蛋白表达较对照组减少。 结论脉冲强磁场具有促进NCSs向神经元分化,并抑制其向星形胶质细胞分化的作用。 相似文献
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阿托伐他汀钙对慢性心力衰竭患者血浆内皮素及心功能的影响 总被引:1,自引:0,他引:1
目的 观察阿托伐他汀钙对慢性心力衰竭患者血清内皮素(ET)及心功能的影响。方法选择98例慢性心力衰竭(NYHA分级Ⅱ~Ⅳ级)患者随机分成2组,治疗组(49例)和对照组(49例),对照组给予强心、利尿、扩血管等常规治疗,治疗组在此基础上加用阿托伐他汀钙20mg,晚饭后顿服,治疗12月,治疗前后检测血清内皮素-1(ET-1)、总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL—C)、高密度脂蛋白胆固醇(HDL-C)和左室射血分数(LVEF)情况并进行比较。结果 治疗组与对照组比较,治疗后血清ET-1、TC、TG、LDL.C均有不同程度的降低,左室射血分数(LVEF)则有明显提高。2组比较,差异有统计学意义(P〈0.05或P〈0.01)。结论阿托伐他汀钙能降低血浆ET-1水平,改善心脏功能,有益于慢性充血性心力衰竭的冶疗。 相似文献
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相信去医院看病的人总有一个体会,就是早上要比医院门诊开放至少提前两个小时去排队才能挂到当天的专家号,如果是大医院,则还要忍受不少票贩子跟你挤来挤去.用市场经济的观点来看,现在的大医院应该说是典型的卖方市场,患者有对服务态度方面的不满,相信大部分只能往自己肚里咽。 相似文献
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