氢氧化钙预防根管治疗期间急症的临床观察

目的 观察氢氧化钙根管内封药对预防根管治疗期间急症的临床疗效。方法 使用氢氧化钙及传统根管内消毒药物樟脑对氯酚、甲醛甲酚根管内封药，观察疼痛指数并比较其根管治疗期间急症的发生率。结果 氢氧化钙封药后根管治疗期间急症的发生率最低，与传统药物相比，其差异有统计学意义。结论 氢氧化钙根管内封药。对预防根管治疗期间的急症有较好的作用。     

浅谈电子文档的原始性和真实性及法律证据作用

该文针对电子文件或档案的原始性真实性与电子档案的法律证据作用,参考了最新的研究结果,并提出了自己的思考,逐步探讨了它们的涵义及关系.     

A Novel Application of the MIRC Repository in Medical Education

Medical students on the radiology elective in our institution create electronic presentations to present to each other as part of the requirements for the rotation. Access was given to previous students presentations via the web-based system, Medical Imaging Resource Center (MIRC) project, created and supported by the Radiological Society of North America (RSNA). RadPix Power 2 MIRC (Weadock Software, LLC, Ann Arbor, MI) software converted the Microsoft PowerPoint (Redmond, WA) presentations to a MIRC-compatible format. The textual information on each slide is searchable across the entire MIRC database. Future students will be able to benefit from the work of their predecessors.     

A bioinformatics pipeline for high-throughput microbial multilocus sequence typing (MLST) analyses

Multilocus sequence typing (MLST) analysis for semi-routine applications is hindered by the downstream, manually intensive steps of processing the raw sequence data files. This report describes the development of an MLST pipeline that automates DNA sequence editing and analysis in order to significantly reduce the time required for processing data. Validation using a pneumococcal dataset revealed complete agreement between the results generated by manual and automated workflows. The MLST pipeline was developed for both double-strand and single-strand sequencing.     

社区健康信息系统的功能与评价

彭浦社区卫生服务中心建立的健康信息系统,将社区居民健康档案与临床信息一体化。与以往社区卫生信息管理系统相比较,在建立居民健康档案方面,实现了“渠道单一”向“多档合一”的转变;在掌握社区人群健康方面,实现了“静态管理”向“动态管理”的转变;在优化服务流程方面,实现了“环节繁多”向“流程简化”的转变;在监测服务提供行为方面,实现了“事后控制”向“实时控制”的转变;在方便居民诊疗方面,实现了“重复检查”向“结果互认”的转变。     

浅谈网站建设中的一些细节问题

结合“作业管理系统”实例,阐述了网站建设中细节上常见的技术问题,引出这些问题涉及的基本概念及给出适用的处理方法,并做出相应的结论。     

La risoluzione delle complessità nei ritrattamenti

ObjectivesThe aim of this study is to describe the main causes and prevalence of endodontic therapy failures, to promote a diagnostic and prognostic work-up suited to the relevant pathology evolution degree and to suggest appropriate clinical strategies to achieve the expected results.Materials and methodsThrough an extensive review of recent literature, combined with an accurate analysis of clinical cases and epidemiological investigation, it was possible to gather and evaluate the necessary information and data for providing clinical dictates on the solution of endodontic retreatment problems.ResultsThe success percentage of endodontic retreatments is quite variable, ranging from 60% to 85% of treated cases. Such percentage is strongly dependent on two critical variables: the preoperative condition of periapical tissues and the previously provided treatment. A chronic apical periodontitis subjected to inadequate therapy leading to the structural alteration of dental elements, is indeed a crucial factor affecting the retreatment prognosis.ConclusionsAll retreatment procedures should be preceded by an accurate case analysis. Adverse anatomical conditions and periapical bone lesions may predict an unfavorable prognosis and should be taken into great consideration. However, in all other clinical conditions, high success rates can be achieved, ensuring a long-term survival of dental elements.     

根管治疗失败的原因与处理对策

根管治疗后出现根尖周病变或原有根尖周病变持续存在，在临床上往往被认为是根管治疗失败。对于这类根管治疗后病变的处理，首先要明确诊断，确定病因，方能为再治疗时机和方法的决策提供依据。临床上，根管治疗失败多与前次根管治疗的质量、患牙病变的性质及患牙存在伴发病变有关。本文将从上述三方面进行分析，并提出相应的处理对策，为临床医生处理根管治疗后病变提供诊断思路和再治疗方法的选择。     

Evaluation of photodynamic therapy on fibroblast viability and cytokine production

BackgroundThe aim of this study was to evaluate the effects of photodynamic therapy with curcumin (PDT) comparatively to 5% sodium hypochlorite (NaOCl) and saline solution on cell viability and cytokine (IL-1β and IL-6) production by mouse fibroblasts.MethodsSixty seconds of pre-irradiation time with curcumin 500 mg/L and Led wavelength (λ) 480 nm, 72 J cm², for 300 s was used for PDT. Solutions were diluted in culture medium DMEM (1 × 10⁴ cells) and placed into 24-well cell culture plates with mouse fibroblasts L-929. Culture medium was used as control. After 6, 24 and 48 h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MTT) was used to evaluate the cell viability and the supernatant was collected for cytokine evaluation using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by ANOVA and BonFerroni correction (p < 0.05) for MTT and Kruskal–Wallis test and Dunn (p < 0.05) for ELISA.ResultsPDT and saline solution presented low cytotoxic effect similar to the control group (p > 0.05) while 5% NaOCl was more cytotoxic than PDT (p < 0.05) in all periods of time. All materials similarly expressed IL-1β and IL-6 regardless to the experimental period (p < 0.05).ConclusionsPDT with curcumin was not cytotoxic to L929 fibroblasts differently from 5% NaOCl. In all groups occurred similar expression of IL-1β and IL-6.