VEGF和LN在大肠癌中的表达及与大肠癌预后的关系

目的：探讨大肠癌血管内皮生长因子(VEGF)、层黏连蛋白(LN)表达及与大肠癌预后的关系。方法：应用SP免疫组化法检测正常大肠黏膜、大肠腺瘤和大肠癌组织VEGF和LN的表达，并分析二者与大肠癌病理特征和预后的关系。结果：大肠癌VEGF和LN的表达均明显高于大肠腺瘤和正常大肠黏膜(P＜0.01，P＜0.05)。大肠癌VEGF和LN的表达均与淋巴结转移、分化程度、Duke’s分期和术后生存期有关(P＜0.01，P＜0.05)。结论：联合检测VEGF和LN可作为判断大肠癌浸润转移和评估预后的参考指标。     

Deproteinized bone with VEGF gene transfer to facilitate the repair of early avascular necrosis of femoral head of rabbit

Objective: To explore a new method for early avascular necrosis of femoral head (AVNFH) therapy.Methods: Sixty-nine AVNFH New Zealand adult rabbits were randomly divided into three groups with equal number. In Group A, deproteinized bone (DPB) that absorbed with recombinant plasmid pcDNA3.1-hVEGF165 was implanted into the drilled tunnel of necrotic femoral head. In Group B, only DPB was implanted. In Group C, only tunnel was drilled without DPB or plasmid implanted. Femoral head specimens were obtained at postoperative 1, 2, 4, 8, 16 weeks. The expression of VEGF165 and collagen I was detected by immunohistochemistry. Bone formation was detected generally by X-ray. Angiogenesis and the repair of the femoral head were observed histologically.Results: The expression of VEGF 165 could be detected 2 weeks after implantation in Group A, but it was not observed in other groups. The result of collagen I expression had a significantly difference 2, 4 and 8 weeks after operation in Group A from those in other groups (P＜0.01).X-ray results indicated that there was more bone formation in Group A than in other groups. The regenerated capillary vessels staining result of necrotic femoral head in Group A was significantly different from those in other groups at postoperative 2 and 4 weeks (P＜0.01).Conclusions: Transfection ofhVEGF165 gene enhances local angiogenesis and DPB-VEGF compound improves the repair of necrotic femoral head. Deproteinized bone grafting with VEGF gene transfer provides a potential method for the treatment of osteonecrosis.