鼻咽癌患者血清与组织乳酸脱氢酶同工酶谱的初步研究

对鼻咽癌患者血清及组织的乳酸脱氢酶(LDH)同工酶进行测定,发现初诊和复发鼻咽癌病人与正常人的血清酶谱虽然相似,但组织酶谱在患癌病人与慢性鼻咽炎病人之间却存在着显著性差异,放疗前后的组织酶谱亦显著不同。提示有可能将鼻咽组织的LDH同工酶谱分析作为一种辅助性诊断或评价疗效的指标。     

CYTOMORPHOMETRIC ANALYSIS DURING CANCERATION OF NASOPHARYNEAL EPITHELIUM

Cellular morphology was quantitatively analysed by using the point-counting technique In 76 specimens inciuding 18 normal nasopharyngeal epithelia, 16 simple hyperplasia and metaplasia, 18 dysplasia and 24 untreated nasopharyngeal carcinoma (NPC). Altogether 19 morphometric parameters of the cell were measured and calculated. THe results showed that with malignant transformation of nasopharyngeal epithelium, the great majority of the cellular morphometric parameters gradually increased in numerical values while no marked difference was shown between normal nasopharyngeal epithelium and simple hyperplasia or metaplasia in terms of cytomorphometric characteristics. Most of the parameters in dysplasla changed significantly as compared with those in other three groups. Cytomorphometric analysis seems to give strong support to the concept that dysplasla is among the transition stages of disease process between normal nasopharyngeal epithelium and NPC. 19 parameters were processed with multivarite analysis an     

Changes of cellular morphology and nuclear DNA content in different nasopharyngeal epithelium from different patients.

Cellular morphology and nuclear DNA content were measured with point-counting technique and flow cytometry (FCM) respectively in normal nasopharyngeal epithelium (NE), tissue adjacent to carcinoma (ATC) and nasopharyngeal carcinoma (NPC). The results showed that all 20 patients with NE show ed diploid, whereas 38 (38%) of 101 patients with NPC, 7 (58%) of 12 patients with recurrent NPC, 6 (30%) of 20 patients with ATC and 2 (33%) of 6 patients with NE positive to EBVCA-IgA test showed nondiploid. A total of 19 morphometric parameters of the cell were measured and calculated. The results showed that with malignant transformation of NE, most of the cellular morphometric parameters gradually increased in numerical values while no marked difference was shown between normal NE and simple hyperplasia or metaplasia in terms of cytomorphometric characteristics. Most of the parameters in dysplasia changed significantly as compared with those normal NE, simple hyperplasia or metaplasia and NPC groups. Cytomorphome     

Changes of nuclear DNA content in different types of nasopharyngeal epithelium.

The unclear DNA content was measured with flow cytometry (FCM) in 167 specimens of normal nasopharyngeal epithelium (NE), adjacent tissue to carcinoma (ATC) and nasopharyngeal carcinoma (NPC). All 20 patients with NE showed diploid, whereas 38 (38%) of 101 patients with NPC, 7 (58%) of 12 patients with recurrent NPC, 6 (30%) of 20 patients with ATC and 2 (33%) of 6 patients with NE, positive to EBVCA-IgA test, showed nondiploid. In addition, cellular proliferation index (PI) of diploid NPC and diploid ATC,though differred nonsignificantly, was significantly increased as compared with that of NE (P<0.01). The rate of cervical metastasis in nondiploid NPC was significantly higher than that in diploid NPC (P<0.025).     

鼻咽上皮癌变过程中超微结构的观察

本文对正常鼻咽上皮,及其单纯增生或化生、异型增生或化生,以及鼻咽癌细胞的超微结构进行观察。发现从正常鼻咽上皮到癌变,细胞的异型性逐渐显著,分化程度逐渐下降。单纯增生或化生鼻咽上皮与正常者比较变化显著。提示对于异型增生或化生中癌变可能性较大者,需考虑抗癌治疗。表明对异型增生或化生根据癌变可能性的大小进行分类具有十分重要的临床意义。     

形态测量术在肿瘤研究中的应用

本文对形态测量术的概念、方法、测定的基本参数以及在肿瘤研究方面应用的文献资料进行综述。形态测量术能对组织、细胞及其结构形态进行定量分析.目前这一新技术正日益受到广大肿瘤研究者的重视。     

鼻咽癌患者放射治疗前后细胞免疫功能的动态观察

本文应用~3H-TdR 掺入淋巴细胞转化试验,对鼻咽癌患者放射治疗前后的细胞免疫功能进行了动态观察。结果表明,治疗后3个月内细胞免疫功能较治疗前明显降低,以后有所回升,至一年以后接近正常;提示放射治疗对细胞免疫功能有一定的损伤作用,但这种损伤是可逆的。     

Detection of the antibody to Epstein-Barr virus-specific DNase by enzyme-linked immunoelectrotransfer blot technique

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