小型猪一侧失牙及义齿修复后TMJ关节液中IL-1β、TNF-α、TGF—β1含量的变化

目的：检测小型猪一侧失牙及义齿修复后颞下颌关节（temporomandibularjoint，TMJ）关节液中IL-1β、TNF-α、TGF-β1含量，探讨其在颞下颌关节紊乱病中的作用及相互关系。方法：11头小型猪随机分为空白组2头、拔牙组4头和修复组5头，拔牙组和修复组左侧后牙全部拔除，修复组于拔牙后3个月义齿修复；每月采集双侧TMJ关节液，ELISA法测定IL-1β、TNF-α、TGF-β1含量（共6个月）。结果：拔牙组和修复组关节液中IL-1p浓度于第1个月达到最大值，拔牙组于第3个月、修复组于第5个月IL-1β浓度达到最小值；拔牙组于第4个月、修复组于第3个月TNF-α达到最大值；拔牙组和修复组于第3个月TGF-β1浓度达到最大值。结论：IL-1β启动并与TNF—α协同破坏TMJ；TGF-β1在TMJ损伤的修复中发挥重要作用。     

缺氧诱导因子-1α和环氧合酶-2在大鼠放射性口腔黏膜炎中的表达及意义

目的检测缺氧诱导因子-1α（HIF-1α）和环氧合酶-2（COX-2）mRNA及蛋白在大鼠放射性口腔黏膜炎组织中的表达水平．并探讨二者之间的关系。方法建立放射性口腔黏膜炎SD大鼠模型，切取左、右侧颊黏膜，用半定量RT—PCR检测各标本HIF—1α和COX-2的mRNA相对表达值．采用免疫组化SABC法检测HIF—1α和COX-2蛋白在黏膜炎组织中的表达部位及表达强度。结果成功地建立了SD大鼠放射性口腔黏膜炎动物模型：RT—PCR结果显示ROM黏膜组织能够表达HIF—1α mRNA和COX-2 mRNA．而右侧自身对照组基本不表达HIF—1α和COX-2 mRNA；免疫组化结果显示：HIF—1α蛋白和COX-2蛋白在ROM黏膜组织中均能广泛表达，HIF—1α和COX-2蛋白的表达基本处于平行的关系．并且表达的部位和细胞类型也基本一致。结论放射性口腔黏膜炎组织有HIF—1α和COX-2 mRNA及蛋白的表达．两者的表达呈高度相关．HIF—1α和COX-2的表达与放射性口腔黏膜炎的严重程度有关。     

Soluble tumour necrosis factor receptors in synovial fluids from temporomandibular joints with painful anterior disc displacement without reduction and osteoarthritis

The objective of this study was to detect soluble-form tumour necrosis factor receptors (sTNFRs) in temporomandibular joint (TMJ) synovial fluid aspirates, and to compare the sTNFR concentrations between painful anterior disc displacement without reduction and osteoarthritis (ADDwoR/OA) and asymptomatic TMJs.Synovial fluid was sampled from the superior TMJ cavity of 11 painful ADDwoR/OA cases (mean age: 36.9 years) and 10 asymptomatic females (mean age: 24.7 years) by diluted aspiration. The concentrations of sTNFR-I and -II in the synovial fluid were measured using human sTNFR-I and -II enzyme-linked immunosorbent assays. The total protein concentrations in synovial fluids were measured using a bicinchoninic acid protein assay kit. All data were normalised to the total protein concentration of each sample.Two-way factorial analysis of variance and post hoc multiple comparison revealed that: (1). mean normalised sTNFR-I and -II concentrations were higher in TMJ synovial aspirates from ADDwoR/OA patients than from healthy controls; (2). in the ADDwoR/OA patients and the healthy controls, the sTNFR-I concentration in TMJ synovial aspirates was higher than the sTNFR-II concentration; and (3). high TMJ synovial aspirate sTNFR-II seemed to be associated with less TMJ pain and a less restricted range of mouth opening in the ADDwoR/OA patients.The concentrations of sTNFRs in TMJ synovial fluid are higher in the presence of painful ADDwoR/OA, which could modulate intracapsular inflammation.     

Effects of ethanolic extracts of Euonymus alatus on CCl4-induced hepatic fibrosis in mice based on JAK2/STAT3 signaling pathway and its mechanism北大核心CSCD

Aim To explore the mechanism of ethanolic extracts of euonymus alatus on CCl4-induced hepatic fibrosis in mice by regulating JAK2/STAT3 signaling pathway. Methods Sixty C57BL/6J mice were randomly divided into control group,model group,EAL,EAM),EAH,and Silybin(n=10). Except for the control group,mice in other groups were injected with 25% CCl4 of 1.6 mL·kg-1 to induce HF model. Moreover,the positive group was administered 12.6 mg·kg-1 Silybin by gavage once a day,and EAL,EAM,and EAH were administered 72,140 and 280 mg·kg-1 ethanolic extracts of euonymus alatus once by gavage once a day,and the intervention lasted for six weeks. After 6th week,mouse blood was collected,the body weight and liver weight were measured and liver mass index calculated,the liver appearance was observed,and ALT,AST,TNF-α,IL-6,IL-1β were detected in serum. The protein expression levels of collagen Ⅰ,α-SMA,and JAK2/STAT3 signaling pathway-related protein(JAK2,STAT3,p-JAK2,pSTAT3)in mouse liver tissues were detected. Results Compared with the model group,EAM and EAH significantly decreased liver mass index,and the ALT,AST,α-SMA,collagen I levels of serum. After treatment,the liver morphology and structure,cellular inflammatory infiltration,fiber changes and collagen deposition in euonymus alatus intervention group were dose-dependently better than those of the model group. Compared with the model group,the expression of TNF-α,IL-6 and IL-1β in EAM and EAH serum decreased significantly(P<0.05). Compared with the model group,the protein expression levels of JAK2,STAT3,p-JAK2,pSTAT3 in EAM and EAH mouse liver tissues decreased significantly(P<0.05). Conclusion Ethanolic extracts of euonymus alatus have an anti-CCl4-induced HF effect in mice,and its mechanism may be related to the regulation of the JAK2/STAT3 signaling pathway. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

Involvement of Nrf2, JAK and COX pathways in acetaminophen-induced nephropathy: Role of some antioxidants

ObjectivesAcetaminophen (APAP)-induced nephrotoxicity is detrimental consequence for which there has not been a standardized therapeutic regimen. Although, N-acetylcysteine (NAC) is a well-known antidote used in APAP-induced hepatotoxicity, its benefit in nephrotoxicity caused by APAP is almost lacking. This study aimed to compare the possible protective effect of thymoquinone (TQ), curcumin (CR), and α-lipoic acid (α-LA), either in solo or in combination regimens with that of NAC against APAP-induced renal injury.Design and methodRats were divided into nine groups; control group, APAP intoxicated group (1000 mg/kg; orally), and the remaining seven groups received, in addition to APAP, oral doses of NAC, TQ, CR, α-LA, CR plus TQ, TQ plus α-LA, or CR plus α-LA. The first dose of the aforementioned antioxidants was given 24 h before APAP, and then the second dose was given 2 h after APAP, whereas the last dose was given 10 h after administration of APAP.ResultsTreatment with APAP elevated kidney markers like serum uric acid, urea, and creatinine. In addition, it increased the serum level of tumor necrosis factor alpha (TNF-α), interleukin-1beta (IL-1β) and thiobarbituric acid reactive species (TBARS). Also, the protein expression of renal janus kinase (JAK) and cyclooxygenase (COX)-2 were all upregulated by APAP. In contrast, the expression of Nrf2 and the renal levels of superoxide dismutase and glutathione were downregulated. Treatment with the indicated natural antioxidants resulted in amelioration of the aberrated parameters through exhibiting anti-inflammatory, antioxidant and free radical-scavenging effects with a variable degree.ConclusionThe combined administration of CR and TQ exerted the most potent protection against APAP-induced nephrotoxicity through its anti-inflammatory and free radical-scavenging effects (antioxidant) which were comparable to that of NAC-treatment.     

Nd:YAG激光联合曲安奈德治疗口腔黏膜下纤维化的临床研究

  目的  采用Nd:YAG激光联合曲安奈德治疗口腔黏膜下纤维化患者,观察治疗后纤维化组织的修复、愈合及病情进展情况。  方法  选取2018年6月—2019年10月于杭州师范大学附属医院口腔科就诊的80例口腔黏膜下纤维化患者,采用随机数字表法分为观察组与对照组,每组40例。对照组患者使用曲安奈德注射液治疗,观察组在此基础上使用Nd:YAG激光治疗,2组均持续治疗9周。2组患者治疗前后采用视觉模拟评分法(VAS)评分进行评价,并测量张口程度和口腔黏膜病损面积;采用酶联免疫吸附法(ELISA)测定血清血管内皮生长因子(VEGF)、血小板反应蛋白(TSP)、基质金属蛋白酶-2(MMP-2)水平及血清IL-6、TNF-α水平。  结果  治疗后,对照组口腔黏膜病损面积为(1.47±0.62)cm2,观察组为(0.86±0.23)cm2,观察组口腔黏膜病损面积小于对照组(P < 0.05);2组患者血清VEGF均升高,观察组高于对照组[(0.75±0.19)pg/mL vs.(0.61±0.23)pg/mL, P < 0.05],而血清TSP、MMP-2、IL-6和TNF-α水平均降低,观察组低于对照组(均P < 0.05)。  结论  Nd: YAG激光联合曲安奈德可有效缓解口腔黏膜下纤维化患者口腔灼痛,抑制纤维化发展,并促进纤维化组织的修复与愈合,控制病情进一步发展。      

针刀整体松解术配合手法对膝骨性关节炎患者血清TNF-α、MMP-13及SOD的影响

目的:观察针刀整体松解术配合手法对膝骨性关节炎(knee osteoarthritis,KOA)患者血清TNF-α、MMP-13及SOD的影响。方法:选取2015年3月—2016年4月十堰市中医医院针灸科治疗的KOA患者130例,随机分为观察组和对照组,每组65例。观察组给予针刀整体松解术[2]配合手法治疗,对照组给予电针治疗。结果:观察组治疗后TNF-α、MMP-13、SOD水平均优于对照组(P0.01)。结论:针刀整体松解术配合手法可以改善KOA患者血清中TNF-α、MMP-13、SOD水平。     

补中益气汤对脑缺血再灌注大鼠神经功能及梗死灶IL-1β和TNF-α表达的影响

目的:观察补中益气汤对脑缺血再灌注大鼠神经功能及梗死灶白细胞介素1β(interleukin-1β,IL-1β)、肿瘤坏死因子α(Tumour necrosis factor-α,TNF-α)表达的影响。方法:将60只SD大鼠随机分为假手术组、模型组、尼莫地平组、补中益气汤高剂量组、补中益气汤中剂量组、补中益气汤低剂量组,每组各10只。假手术组和模型组给予蒸馏水0.003 m L·(g·d)~(-1),尼莫地平组给予尼莫地平混悬液0.003 m L·(g·d)~(-1),补中益气汤高剂量组、补中益气汤中剂量组、补中益气汤低剂量组分别给予临床用药量的25倍、20倍、15倍水煎浓缩液灌胃,用量均按人与大鼠体表面积等效剂量折算,各组等容灌胃每日2次,灌胃6 d后,第7天采用Longa等线栓法复制大脑急性脑缺血再灌注损伤模型,在造模后12h进行神经功能缺损评分,测量脑指数,测定IL-1β、TNF-α含量,光镜下做脑组织病理形态学观察。结果:模型组大鼠脑指数降低、神经体征评分明显高于假手术组(P0.05);与模型组比较,给药组大鼠脑指数降低、神经体征明显改善,差异具有统计学意义(P0.01);与模型组比较,给药组大鼠脑组织IL-1β,TNF-α的含量明显降低,差异具有统计学意义(P0.01);与尼莫地平组比较,补中益气汤高剂量组脑指数明显降低,脑组织IL-1β、TNF-α的含量降低明显,差异均有统计学意义(P0.05)。病理组织学检查发现模型组大鼠脑组织出现不同程度的水肿,神经细胞轴索肿胀、断裂等缺血样改变,给药组大鼠上述病变明显减轻。结论:补中益气汤能明显改善脑缺血再灌注大鼠神经体征,可下调脑指数、降低脑组织IL-1β,TNF-α的含量,对脑缺血再灌注大鼠脑组织病理形态有较强的改善作用。     

Characterization of synovial fluid from periprosthetic infection in revision total joint arthroplasty by single-molecule microscopy

Periprosthetic joint infection is among the most common and severe complications in total joint arthroplasty. Today, a combination of different methods is used for diagnosis because no single method with sufficient sensitivity and specificity is available. In this study, we explored the usability of single-molecule microscopy to characterize synovial fluid samples from periprosthetic joint infections. Patients (n = 27) that needed revision arthroplasty underwent the routine diagnostic procedures for periprosthetic joint infection of the University Hospital in Bonn. Additionally, the diffusion rate of two probes, dextran and hyaluronan, was measured in small volumes of periprosthetic synovial fluid samples using single-molecule microscopy. To evaluate the suitability of single-molecule microscopy to detect PJI the AUC for both markers was calculated. The diffusion rate of hyaluronan in periprosthetic synovial fluid from patients with septic loosening was faster than in samples from patients with aseptic loosening. Single-molecule microscopy showed excellent diagnostic performance, with an area under the receiver operating characteristic curve of 0.93, and allowed the detection of periprosthetic joint infection in patients that would be challenging to diagnose with current methods. For the first time, single-molecule microscopy was used to detect periprosthetic joint infection. Our results are encouraging to study the value of single-molecule microscopy in a larger patient cohort. The speed and accuracy of single-molecule microscopy can be used to further characterize synovial fluid, potentially allowing intraoperative diagnosis of periprosthetic joint infections in the future.