bFGF和CD105在胃癌组织中的表达及其生物学行为

目的 探讨缄性成纤维细胞生长因子(bFGF)和CD105在胃癌组织中的表达及其生物学行为.方法 采用免疫组化检测60例胃癌组织及20例正常胃组织中bFGF与微血管密度(MVt))的表达和胃癌细胞增殖指数(SPF).用TUNEL原位凋亡法检测胃癌组织中胃癌细胞凋亡,计算凋亡率(AR).结果 胃癌组织中bFGF蛋白表达、MVD、SPF明显高于正常胃组织,而AR低于正常胃组织.bFGF、MVD和SPF呈正相关(r=0.48、0.51、0.46,P<0.05).bFGF和MVD与AR呈负相关(r=-0.44、-0.47、0.43,P<0.05).结论 bFGF可能通过上调MVD的表达,从而促进胃癌细胞增殖,抑制胃癌细胞凋亡.     

Enhanced levels of cow's milk antibodies in infancy in children who develop type 1 diabetes later in childhood

Background: Early exposure to cow’s milk (CM) proteins have been implicated in the pathogenesis of type 1 diabetes (T1D). Objective: We analyzed the development of the humoral immune response to dietary CM proteins in early childhood and its relation to later T1D. Subjects and methods: We studied a subgroup of 94 children randomized to be weaned to a CM‐based infant formula in the trial to reduce insulin‐dependent diabetes mellitus in the genetically at risk (TRIGR) pilot study. All subjects carried human leukocyte antigen‐conferred T1D susceptibility and had an affected first‐degree relative. After 7 years of follow‐up, 8 subjects had progressed to T1D, 15 had at least one disease‐associated autoantibody, and 71 remained autoantibody negative (controls). Immunoglobulin (Ig) G and IgA class antibodies to whole CM formula, beta‐lactoglobulin (BLG), bovine serum albumin, and alpha‐casein and IgG antibodies to bovine insulin (BI) were measured with enzyme‐linked immunosorbent assays from sequential samples. Results: The children with later T1D showed increased IgG levels to BLG from 3 to 18 months of age (p = 0.028) and enhanced IgA levels to CM formula at the age of 9 months (p = 0.022) compared with controls. In the children with an affected father or sibling, IgG antibodies to BI were higher in autoantibody‐positive subjects than in autoantibody‐negative subjects at 18 months of age (p = 0.022). Conclusion: An enhanced humoral immune response to various CM proteins in infancy is seen in a subgroup of those children who later progress to T1D. Accordingly, a dysregulated immune response to oral antigens is an early event in the pathogenesis of T1D.     

行为干预辅助治疗学龄前儿童弱视的疗效

目的 探讨行为干预对学龄前儿童弱视疗效的影响.方法 采用Achen-bach儿童行为量表对120例4～6岁学龄前儿童进行评定分析.将80例弱视儿童分为研究组(40例)和弱视对照组(40例),其余40例无弱视的儿童为健康对照组.弱视儿童进行弱视治疗,并对研究组采用行为干预治疗1年以上,然后与弱视对照组进行行为因子和弱视疗效的比较.结果 研究组行为干预治疗后社交退缩和攻击2个共同行为因子的评分与弱视对照组的差异有统计学意义(P＜0.05).研究组弱视治愈率和视力提高程度与弱视对照组的差异有统计学意义(P＜0.01).研究组立体视恢复率较弱视对照组高,差异有统计学意义(P＜0.01).结论 弱视儿童存在心理行为问题,行为干预治疗有助于提高弱视治疗的疗效.     

乳腺超声若干临床常见问题专家共识(2018版)解读

【摘要】 《乳腺超声若干临床常见问题专家共识（2018版）》是针对超声BI RADS标准在中国应用过程中引发的热点问题,中国超声医学工程学会第三届浅表和血管专业委员会于2018年多次组织专家进行现场及线上的热烈讨论,最终就二十个问题形成统一观点,发表“共识”的中英文版。该共识为国内同行提供乳腺超声报告的规范,其英文版也代表中国声音,引起国际关注,旨在乳腺超声报告规范国际化。     

Comprehensive ADL Outcome Measurement after Stroke: Rasch Validation of the Lucerne ICF-Based Multidisciplinary Observation Scale (LIMOS)

ObjectiveTo establish Rasch validation of the Lucerne International Classification of Functioning, Disability and Health (ICF)-based Multidisciplinary Observation Scale (LIMOS) in stroke. LIMOS measures the level of assistance in daily life activities related to motor function, communication, cognition, and domestic life. Rasch Measurement Theory (RMT) transforms an ordinal scale into an interval scale and thus the Rasch-based LIMOS scale captures a more accurate improvement of functional outcomes via Rasch-transformed scores.DesignCross-sectional study design.SettingNeurorehabilitation center, Luzerne, Switzerland.ParticipantsWe recruited participants with stroke (N=407; age=63.2±16.0y; n=157 women) through consecutive sampling.InterventionsNot applicable.Main Outcome MeasuresParticipants were assessed with LIMOS at admission and discharge of rehabilitation. We used RMT to evaluate overall model fit, response dependency, floor and ceiling effect, reliability, and differential item functioning (DIF) for sex, age, type, and time of stroke on the 4 LIMOS subscales using the Rasch Unidimensional Measurement Model (RUMM) 2030 program.ResultsThe Rasch-based LIMOS subscales fit the Rasch model after reducing and rescoring items: motor (from 20 to 18 items), communication (5 items), cognition (from 15 to 13 items), and domestic life (5 items). There was no floor or ceiling effect. Some artificial DIF was identified. Scoring at discharge was dependent on the scoring responses at admission, which means that without applying a correction factor to the discharge scores, there was an underestimation of change in scores between admission and discharge, ranging from 0.24 to 0.97 logits (10.79%-49.24%) on the different subscales.ConclusionsThe Rasch-based LIMOS scale is recommended to measure functional outcome in people with acute or chronic stages of ischemic or hemorrhagic stroke.     

3DCPA联合TIRADS标准在甲状腺结节良恶性诊断中的价值

【摘要】 目的 探讨3D CPA联合TI RADS在甲状腺结节良恶性诊断中的价值。方法 收集2013年12月~2015年12月在遵义医学院附属医院行甲状腺手术的患者98例共105个甲状腺结节（良性50个,恶性55个）,回顾术前常规二维超声检查及3D CPA检查,所有甲状腺结节均按照TI RADS标准进行分类。以病理结果为金标准,比较3D CPA与TI RADS标准对甲状腺结节的良恶性诊断价值及联合两种方法对甲状腺结节良恶性的诊断价值。结果 3D CPA结果：50个良性病灶血流分布在周边的占36个（72.0%）、内外都有14个（28.0%）;血管走行规则43个（86.0%）、血管走行不规则为7个（14.0%）;空间分布对称为44个（86.0%）,空间分布不对称为6个（14.0%）。55个恶性病灶血流分布在周边的占5个（9.1%）、内外都有50个（90.9%）;血管走行规则为6个（10.9%）,血管走行不规则为49个（891%）;空间分布对称为18个（32.7%）,空间分布不对称为37个（67.3%）。良性结节和恶性结节在3D CPA声像图3组指标相比均具有明显差异（P<0.05）。3D CPA与TI RADS之间比较：SEN、SPE、PPV、NPV及ACC均有统计学差异（P<0.05）;.二种方法结合后灵敏度准确度明显高于单一方法。 结论 3D CPA中血流分布部位、血管走行、空间分布对诊断甲状腺结节良恶性有较好的临床意义,其中血管走行的敏感度、特异和准确度最高;. TI RADS分类标准可以对甲状腺结节超声描述起到规范化、标准化的作用,对甲状腺结节提供了风险性评估方案;3D CPA与TI RADS分类标准联合应用可提高诊断甲状腺结节良恶性的灵敏度及准确率。     

p21和p53在肾间质纤维化大鼠中表达的研究

目的动态观察p21和p53在单侧输尿管梗阻肾间质纤维化大鼠模型中的表达,以及依那普利对其表达的调节。方法将SD大鼠随机分为假手术组（SOR组）、单侧输尿管结扎组（UUO组）和依那普利治疗组（EnT组）,每组30只。用Masson染色的方法动态观察梗阻侧肾脏病理变化,用RT—PCR的方法检测梗阻肾皮质p21mRNA和p53mRNA的变化;并观察依那普利对肾皮质p21和p53表达的调节。结果在UUO大鼠模型早期,肾皮质p21mRNA和p53mRNA的表达即有明显增高,随着梗阻时间的延长,p21和p53的表达逐渐增强,二者具备相关性;依那普利能明显抑制p21和p53的表达。结论p21和p53在UUO大鼠肾皮质呈高表达,依那普利可抑制其表达。p21可能通过p53参与介导了。肾小管间质纤维化的发病过程。