CD4+CD25+调节性T细胞与免疫耐受的研究进展

CD4+CD25+调节性T细胞的主要功能是抑制自身反应性T细胞,其在维持机体T细胞内环境稳定,调节和保持对自身抗原耐受之间的平衡以及移植免疫耐受方面具有重要作用.本文主要就CD4+CD25+调节性T细胞的功能、作用机制以及免疫抑制药物对调节性T细胞的影响等研究进展进行综述.     

三氧化二砷治疗急性早幼粒细胞白血病的机制与不良反应

三氧化二砷 (As2 O3)是砒霜的主要成分 ,在治疗急性早幼粒细胞白血病 (APL)的过程中显示出卓越的疗效〔1〕。十九世纪中叶 ,欧洲化学家兼药物学家Lisscwer首先用 1 %亚砷酸钾 (Fowler液 )治疗慢性粒细胞白血病。 1 971年 3月哈尔滨医科大学第一临床医学院率先试用As2 O3治疗APL ,取得临床上的成功 ,使As2 O3成为国际血液肿瘤学界研究的新热点 ,成为继全反式维甲酸之后治疗APL的有效药物〔1 ,2〕。As2 O3治疗APL的可能机制是通过巯基依赖性途径 ,诱导线粒体跨膜电位下降及凋亡信号传导的分子活化 ,使恶性肿瘤细胞发生凋亡 ;同时延缓…     

奶牛口蹄疫免疫不良反应的防治

疫苗免疫是控制奶牛重大传染病的最有效方法之一，但在免疫过程中，经常发生过敏、厌食等应激反应的病例，给奶牛饲养户造成了很大的经济损失，同时，因不良应激反应导致奶牛饲养户对疫苗免疫产生抵触情绪，给动物防疫工作带来了很大困难。     

表达谱基因芯片筛选体外循环前后心肌细胞因子的变化

Objestive Systemic inflarmmation may be triggered by injury, hypothermia, ischemia-reperfusion and the contact of the blood with foreign body during cardiopulmonary bypass (CPB). To determine the application values of gene chip technique in the clinical practice and the study of cardiovascular stagery, as well as to provide clues to the study of inflammatory responess during CPB, microarry for gene expression profiles was used to identify the differences in the gene expression of myocardium between pre-and post- CPB. Methods Six adult patients who underwent CPB from March to May in 2003 were involved. Samples of right atrium were col- lected before and at immediate end of CPB. BD AtlasTM cDNA Expression Arrays was used to identify the differences in the gene ex- pression of cytokines. The results were compared with that of semi-quantative RT-PCR. Resellts The mean age of 6 patients (5 males and 1 female) was (32.67± 11.72) years. The baseline heart function was gradeⅡin 3 cases and grade Ⅲ in 3 other cases. The baseline left ventricular ejection fraction(LVEF)was (58.17±7.91)%. The mere duration was (91.67±43.88) minutes for CPB and was (58.67±43.46) minutes for aorta blocking. The minimum nasopharynx/rectal temperture was (29.37±1.90)℃/ (32.15±1.52)℃. Gene expression profiles of cytokines in the myocardium pre- and post-CPB were analysed successfully. The ex- pression of IL-6, IFN-γ,Wnt5a, TNFRSF1B, a member of tumor necrosis factor receptor superfamily, PIGF and MFNG in the myo- cardium were unpregulated after CPB. Conclusion Microarray technique is applicable in the study of cytokines changes dying CPB. cDNA microarray identified pleliminarily the differences in the gene expression between pre- and post-CPB. These genes may be in- valved in inflammation and other psthophysiological responses incuced by CPB. The myocardiym is probably one of the major sources of cytokines during CPB. Further study may be helpful in understanding the llngthe development of inflammation during CPB, and eventually, reducing the post-operative complications.     

75％酒精治疗锝亚甲二磷酸盐所致静脉炎的疗效观察

目的观察75%酒精治疗锝亚甲二磷酸盐(99Tc-MDP)所致静脉炎的疗效。方法对应用99Tc-MDP治疗类风湿性关节炎所致静脉炎的30例患者采用75%酒精纱布湿敷,测量患者湿敷前后疼痛程度、起效时间及预后等。结果21名患者湿敷4～5h有效,9名患者当天湿敷有效,继续敷用2～3d后痊愈,有效率100%;湿敷前后患者疼痛指数分别为5.6分、3.2分,治疗前后比较,差异有统计学意义(P<0.05);患者湿敷后平均46.2min疼痛开始缓解。结论75%酒精湿敷可以明显改善因99Tc-MDP静脉输入所致的静脉炎。     

甲氨蝶呤致化学性蛛网膜炎1例

病例患儿，女，11岁。2年半前被确诊为急性淋巴细胞白血病L2型，现回院化疗。用甲氨蝶呤12.5mg，地塞米松5mg，阿糖胞苷35mg，生理盐水5ml鞘内注射，2～3小时后患者出现头痛，呕吐，发热，最高体温达40．1℃，热退后症状缓解，查体颈项强直阳性，被确诊为化学性蛛网膜炎。     

头孢菌素的二硫醒样副作用

<正>近年来,随着各种药物的广泛应用,药物的不良反应也日渐增多。头孢菌素类的抗菌素,因抗菌谱广、耐酸、耐酶,毒性小、副作用少,临床运用很广泛。本文对2001年9月-2005年9月我院遇到应用头孢菌素类药(主     

高浓度氯化钾微量泵注射治疗严重低钾血症

目的：探讨低钾血症的治疗方法。方法：用微量泵静脉注射高浓度钾对54例严重低血钾症患者进行补钾治疗。结果：全部病例48小时内纠正低钾，没有不良反应。结论：本方法操作简单易行，疗效确切满意，值得推广。