Antibacterial effect of ozone on cariogenic bacterial species

Objective

The aim was to evaluate the antibacterial effect of ozone on cariogenic bacterial species with and without the presence of saliva and a possible effect on the salivary proteins.

Methods

Suspensions of Actinomyces naeslundii (ACTCC 12104^T), Lactobacilli casei (N CTC 151) and Streptococcus mutans (NCTC 10449), in salt buffer or in saliva, were exposed to ozone gas delivered by the ozone generator Healozone™ 2130C. Aliquots of the suspensions were taken after 10, 30 and 60 s ozone exposures and cultivated on agar plates. Initial number of bacteria per ml was 8.0 × 10⁷ (SD 2.2 × 10⁷) (A. naeslundii), 1.0 × 10⁸ (SD 3.1 × 10⁶) (L. casei) and 1.0 × 10⁸ (SD 7.0 × 10⁵) (S. mutans), respectively. The proteins were separated by SDS electrophoresis and visualized by silver staining.

Results

In salt buffer 92%, 73% and 64% of the initial numbers of A. naeslundii, S. mutans and L. casei, respectively, were killed already after 10 s ozone exposure, while approximately 99.9% of the bacteria were dead after a 60 s exposure. After 10 and 30 s, but not after 60 s exposure to ozone, S. mutans and L. casei were less efficiently killed in saliva compared to the salt buffer. Various saliva proteins were degraded by ozone after a 60 s exposure.

Conclusions

The cariogenic species S. mutans, L. casei and A. naeslundii were almost eliminated following 60 s of ozone treatment. This killing was reduced in the presence of saliva although increasing the ozone application time to 60 s overcame these reductants in saliva. Detection of altered salivary proteins indicates that saliva components constitute additional targets for ozone.     

针灸推拿配合臭氧治疗肩周炎

目的：探讨针灸推拿配合臭氧治疗肩周炎的有效方法.方法：对78例肩周炎患者运用针灸推拿配合臭氧治疗.结果：治疗78例,痊愈45例,显效20例,有效13例,无效0例,有效率100%.结论：针灸推拿配合臭氧治疗肩周炎效果显著.     

靶点射频消融联合臭氧盘内注射治疗腰椎间盘突出症临床体会

目的：观察突出物靶点射频消融联合臭氧盘内注射治疗腰椎间盘突出症的临床疗效.方法：确诊为腰椎间盘突出症的患者100例,根据入院后患者意愿自行选择分成两组.A组50例行胶原酶盘外治疗组;B组50例突出物靶点射频消融联合臭氧盘内注射治疗组.临床疗效采用改良MacNab评分及VAS评分,分别评价术后第4天,1个月,6个月,12个月的疗效.结果：术后有效率分别为：A组84.7%,90.3%,92.4%,94.2%;B组：92.8%,93.0%,93.7%,96.3%,组间比较差异显著（P〈0.05）.结论：突出物靶点射频消融联合臭氧盘内注射是治疗腰椎间盘突出症的有效方法,6个月疗效远高于胶原酶盘外治疗.     

不同浓度医用臭氧对实验大鼠膝关节组织结构安全性评价

目的 观察不同浓度医用臭氧对实验大鼠膝关节软骨组织的病理变化影响,以评估对正常软骨、滑膜组织的氧化应激程度.方法 32只实验大鼠随机分为A、B、C、D4组,每组各10只随机选择一侧膝关节腔内注射医用臭氧气体.A组为浓度10 mg/L,B组为30 mg/L,C组浓度为50 mg/L,D组浓度为80 mg/L,4组分别注射1ml气体.注射医用臭氧后2d、2周、4周后分别对膝关节软骨、滑膜取活组织行病理学光镜检查,对侧膝关节软骨取活组织作空白对照组.结果 与空白对照组比较,病理光镜下显示,关节内注射10 mg/L医用臭氧2d、2周、4周后软骨、滑膜组织未见明显异常.50 mg/L浓度的医用臭氧对软骨、滑膜的氧化作用比30mg/L的稍强,80 mg/L浓度的医用臭氧氧化作用要强许多,软骨在注射80 mg/L浓度的医用臭氧后2周后出现不同程度的病理改变,并且4周后软骨出现组织结构破坏.结论 当医用臭氧浓度达到50 mg/L以上时,开始对软骨、滑膜细胞产生损伤,80 mg/L浓度的医用臭氧出现组织结构破坏.提示在临床应用中30 mg/L的医用臭氧浓度是安全的.     

虎蛇解毒方配用三氧治疗慢性乙型肝炎的临床研究

目的探讨虎蛇解毒方配用体外循环三氧对慢性乙型肝炎患者肝功能的保护作用及对HBV复制的抑制作用。方法以清热凉血解毒,疏肝健脾补肾为法,自拟"虎蛇解毒方",观察其配用体外循环三氧对慢性乙型肝炎患者HBV复制的抑制作用、肝功能的保护作用以及患者免疫功能的变化,并设干扰素组及常规护肝组进行疗效比较。结果虎蛇解毒方配用体外循环三氧组HBeAg、HBV DNA转阴率、ALT复常率及临床有效率分别为46.9%、67.5%、85%及85%;干扰素组HBeAg、HBV DNA转阴率、ALT复常率及临床有效率分别为32.3%、34.2%、71.7%及60.53%;常规护肝组HBeAg、HBV DNA转阴率、ALT复常率及临床有效率分别为5.9%、7.1%、50%及23.81%。经统计学处理,虎蛇解毒方配用三氧治疗组ALT复常率、HBV DNA、HBeAg的转阴率、免疫功能指标CD3、临床效果较常规护肝组明显提高(P0.01),与干扰素组比较,ALT复常率、HBeAg的转阴率差异无统计学意义(P0.05);但HBV DNA临床效果、免疫功能指标CD3差异有统计学意义(P0.05或P0.01)。结论虎蛇解毒方配用体外循环三氧疗法对HBV有一定的抑制作用,对肝功能及免疫功能有改善作用,并可作为治疗慢性乙型肝炎的一种重要的治疗方法 。     

臭氧预处理对内毒素血症大鼠肾脏水通道蛋白的影响

目的研究臭氧预处理对于脂多糖诱导的急性肾功能衰竭(ARF)时水通道蛋白2(AQP-2)的影响。方法 30只雄性Wistar大鼠随机分成3组:空白对照组(CL组)、内毒素处理组(LP组)、臭氧预处理组(OP组)。OP组每天在同一时间腹腔注射1次臭氧(1mg/kg,浓度50μg/mL),每次约4.4～5mL,连续10d。LP组每天注射相同体积的空气。LP组、OP组于最后1次预处理后24h腹腔注射脂多糖10mg/kg。脂多糖注射4h后处死大鼠留取肾脏标本,免疫组织化学染色观察AQP-2表达及肾脏组织核转录因子(NF)-κB的活化程度。结果与CL相比,LP组与OP组AQP-2表达均下降(P<0.01),但OP组下降低于LP组(P<0.01)。CL组NF-κB的活化极少,而LP组与OP组NF-κB活化程度均增高(P<0.01),但LP组升高较OP组更为明显(P<0.01)。结论臭氧预处理可以抑制肾脏AQP-2的下降,减轻肾脏组织的损伤。其机制与臭氧抑制NF-κB的活性,调节炎症的产生和消退,并且保护细胞的氧化还原状态有关。     

弯套针旋转多向注射法注射臭氧治疗腰椎间盘突出症的电生理评价

目的:比较弯套针旋转多向注射法及常规注射方法注射臭氧治疗腰椎间盘突出症(LDH)的电生理改变。方法:自2005年5月至2009年6月,选择100例LDH患者,其中男68例,女32例;年龄25～58岁,平均为44岁;病程3个月～8年,平均8.8个月。全部患者按就诊顺序编号,并运用DPS软件完全随机地分成观察组(A组)和对照组(B组),每组50例。A组采用弯套针旋转多向注射法注射臭氧至病变的椎间盘内治疗,B组采用常规注射方法。所有患者于治疗前及治疗后3个月时进行电生理检查,检测患肢主要肌群及相应节段的椎旁竖脊肌的肌电图,计算运动单元电位(MUP)时限和多相比,并检查双下肢胫神经H反射,记录异常例数及H波潜伏期。结果:两组治疗后出现异常肌电图的肌肉数均有不同程度减少,但A组减少更显著(P0.05或0.01);两组患者治疗后患侧下肢肌群的MUP时限和多相比均减少,并接近正常水平,经统计学分析均有统计学意义(P0.01),且两组间比较,A组变化优于B组,具有统计学意义(P0.05或0.01)。治疗前两组间H反射异常例数比较无统计学差异,治疗后A组明显少于B组(P0.05)。两组治疗后H波潜伏期均明显缩短且趋于正常,但A组比B组更明显,差异有统计学意义(P0.05)。结论:神经电生理的异常表现可以反映神经根受压损害的程度,是判断神经肌肉功能的客观指标之一。运用弯套针旋转多向注射法比常规注射方法注射臭氧治疗LDH能更好地改善电生理异常。     

医用臭氧介入治疗椎间盘突出症的临床应用研究

目的探讨应用医用臭氧治疗腰椎间盘突出症的临床疗效。方法腰椎间突出症39例,按照CT/MRI表现椎间盘突出症分为3种,分别采取侧后入路方法及小关节内侧缘穿刺方法,均经皮腰椎间盘内注射浓度45μg/ml的医用臭氧10～15ml,退针至椎间孔附件时,向椎旁组织内神经根旁注入臭氧10ml,按照纤维环是否破裂分组研究纤维环破裂与疗效的关系,另对2例椎间盘突出症手术后腰背综合征（FBSS）进行臭氧治疗的疗效观察。结果 39例臭氧治疗腰椎间盘突出症3～6个月观察显示：根据MacNab临床疗效评价标准,总有效率为82.95%,其中显效20例,42.42%,有效12例,40.53%,无效7例,17.05%,其中FBSS治疗效果良好。对于未破裂总有效率66.5%,破裂组总有效率87.1%,差异有统计学意义。另对2例椎间盘突出伴度滑脱病人进行臭氧实验性治疗研究,6个月后临床症状显著改善。结论 39例共48盘椎间盘病人臭氧治疗效果确切,2例FBSS疗效良好,2例滑脱病人效果显著,椎间盘破裂组臭氧治疗明显优于未破裂组。同时对椎间盘突出症臭氧治疗的适应症及禁忌症做进一步分析。     

臭氧与紫外线对普外科换药室空气消毒效果的临床观察

目的观察臭氧与紫外线对普外科换药室空气消毒的效果,以期为临床工作提供理论帮助。方法收集我院普外科换药室120日的消毒时间,分为观察组（60d）与对照组（60d）,观察组应用臭氧进行消毒,对照组应用紫外线进行消毒,于消毒前、后对空气中的菌落进行培养,比较两种方法的差别。结果臭氧与紫外线均能对普外科换药室的空气进行消毒,但是臭氧消毒后菌落数更少。结论臭氧与紫外线对普外科换药室均有明显的消毒作用,但是臭氧的消毒效果更明显,临床可以积极应用。     

Ambient ozone concentration and hospital admissions due to childhood respiratory diseases in New York State, 1991-2001

Background and objective

Respiratory diseases constitute one of the leading causes of ill health among children in New York State (NYS). The current project, a component of the NYS Environmental Public Health Tracking Program, investigated a potential association between ambient ozone (O₃) concentration and childhood respiratory hospital admissions over 11 years in NYS.

Methods

We used a two-stage Bayesian hierarchical model to assess the exposure-disease associations within 11 geographic regions and statewide. The data included total daily hospital admissions due to respiratory diseases for children 0-17 years old from 1991 to 2001 in NYS (N=134,099) and daily ambient O₃ level with different single-day lags. These analyses adjusted for particulate matter ?10 μm in size (PM₁₀), meteorological conditions, day of the week, seasonality, long-term trends, and demographic characteristics.

Results

In 5 of the 11 regions, including the Upper and Lower Adirondacks, Upper Hudson Valley, Staten Island, and New York City, positive associations were found between respiratory hospital admissions and ambient O₃ level 2 days prior to the admission. Applying different statistical methods and sensitivity analysis of PM₁₀ did not alter these findings. When region-specific results were combined, no statewide association was apparent.

Conclusions

Geographic differences were found in the associations between O₃ levels and respiratory hospital admissions among children. In addition, we found that the two-stage model may be an appropriate approach for tracking the health effects of air pollution over time in different geographic areas when heterogeneity of risk factors across regions is present.