Identification of differentially expressed proteins in human stage I lung adenocarcinoma and tumor-adjacent tissues with two-dimension gel electrophoresis profiling

Objective： The aim of this study was to establish reproducible two-dimensional electrophoretic assay used for profiling and identification of differentially expressed proteins in human stage I lung adenocarcinoma and paired normal tumor-adjacent tissue. Methods： The proteins from 12 human stage I lung adenocarcinoma tissues and normal tumor-adjacent tissues were separated using isoelectric focusing electrophoresis （the first dimension） and the subsequent homogeneous SDS-polyacrylamide gel electrophoresis （SDS-PAGE） （the second dimension）. The differentially expressed proteins were determined with PDQuest image analysis software, and identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS） and database searching. Results： The well-reproducible 2-DE gel patterns of human stage I lung adenocarcinoma and normal tumor-adjacent tissues were profiled and 26 differentially expressed proteins uncovered. Nine of these 26 protein spots were cut out from the preparation gels and determined with MALDI-TOF-MS. Searching against the protein database, four candidate proteins were identified. They were 60S acidic ribosomal protein P2, Cathepsin B1, Apolipoprotein A-I precursor, and La 4.1 protein. Conclusion： In this study, high reproducible 2-DE gel protein images of human stage I lung adenocarcinoma and paired normal tumor-adjacent tissues were achieved successfully, and 4 differentially expressed proteins were revealed. These data will be helpful for screen of early biomarker and study of molecular mechanisms of human lung adenocarcinoma.     

辨证施护对2型糖尿病患者生活质量的影响

目的 探讨辨证施护对2型糖尿病患者生活质量的影响.方法 将103例2型糖尿病患者随机分为观察组(59例)和对照组(44例),对照组采用常规护理方法 ,观察组在常规护理的基础上根据疾病证型进行辨证施护.采用2型糖尿痛患者生活质量评定量表评定患者干预前后的生活质量.结果 干预1个月后对照组与观察组疾病、心理功能、满意程度3个维度评分及生活质量总分比较,差异有显著性意义(均P＜0.05).结论 辨证施护能显著提高2型糖尿病患者生活质量,促进其生理和心理的舒适.     

布鲁菌脉冲场凝胶电泳图谱分型研究

我们挑选国内不同年分、不同地点分离的95株布鲁菌（包括19株标准菌株），进行脉冲场凝胶电泳图谱分型的分析研究。     

启事

肿瘤细胞对电离辐射的反应依赖于氧，肿瘤的缺氧区需要更高的辐射量才能达到与氧供正常区相似的生物学效应。在缺氧情况下，肿瘤细胞发生遗传及适应性的改变可以提高其在不利的生理环境下的存活及增殖，其中的一个重要因子就是缺氧诱导因子1（HIFl），它是很多与适应相关的基因的转录因子，如血管生长因子、VEGF、糖转运子（Glut-1）。人肿瘤细胞产生2个生长因子家族专门作用于内皮细胞，包括VEGF家族及血管生成素家族（Ang-1、-2和-4）。     

上颌双侧磨牙形态异常1例

正常上颌第一、第二磨牙有独特的外形特征 ,尤其上颌第一磨牙 ,其牙冠外形和牙根数目都比较恒定。然而 ,笔者最近发现 1例 ,患者的上颌双侧第一磨牙、左侧第二磨牙牙冠及牙合面形态与常见的同名牙有明显差异 ,且牙根数目均为4个。笔者观察其牙冠牙合面形态与牙根数目可能有相关     

口腔门诊临诊晕厥急救52例分析

目的：探讨口腔门诊临诊晕厥常见病因及其防治。方法：回顾性分析在口腔门诊发生的52例晕厥病人临床资料。结果：不同性别、不同临诊经历病人晕厥发生率具有显著性差异。结论：初诊女性患者更易发生临诊晕厥；声音与影象的刺激较疼痛刺激更易诱发晕厥。     

彩色多普勒对脑梗死患者椎动脉供血不足的诊断价值

目的 探讨彩色多普勒超声对脑梗死患者颅外段椎动脉血流的诊断价值.方法 对100例脑梗死患者的椎动脉进行彩色多普勒超声检查并设非脑梗死60例作对照组,观察其形态、结构及血流动力学.结果 100例患者的椎动脉均显示有不同程度的管壁增厚,内径狭窄,血流量减少,阻力指数增高,其中血流量减少、阻力指数增高较对照组有显著性差异(P＜0.05).结论彩色多普勒对有椎基底动脉供血不足的脑梗死患者的诊断提供了血流动力学和形态学的依据.