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51.
Peter D. Kirkland Melinda Gabor Ian Poe Kristie Neale Kim Chaffey Deborah S. Finlaison Xingnian Gu Paul M. Hick Andrew J. Read Therese Wright Deborah Middleton 《Emerging infectious diseases》2015,21(12):2182-2185
Hendra virus occasionally causes severe disease in horses and humans. In Australia in 2013, infection was detected in a dog that had been in contact with an infected horse. Abnormalities and viral RNA were found in the dog’s kidney, brain, lymph nodes, spleen, and liver. Dogs should be kept away from infected horses. 相似文献
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Adithya Sridhar Salvatore Simmini Carla M. S. Ribeiro Caroline Tapparel Melvin M. Evers Dasja Pajkrt Katja Wolthers 《Viruses》2020,12(11)
Animal models and cell lines are invaluable for virology research and host–pathogen interaction studies. However, it is increasingly evident that these models are not sufficient to fully understand human viral diseases. With the advent of three-dimensional organotypic cultures, it is now possible to study viral infections in the human context. This perspective explores the potential of these organotypic cultures, also known as organoids, for virology research, antiviral testing, and shaping the virology landscape. 相似文献
54.
目的探讨肝硬变(LC),原发性肝癌(HCC)发生和发展与HCV及HBV感染的关系.方法HCC28例,LC48例和对照组50例,用ELISA法同步检测HBV及HCV的6项血清标志物(M).结果HCC,LC及对照组HBV_M的阳性率分别为750%,813%和400%;HCV_M的阳性率分别为71%,208%和20%.HCC,LC中HBV_M阴性患者HCV_M阳性率为125%.HCV与HBV重叠感染者占HCC和LC全部患者的132%.结论HCC,LC发生和发展与HBV及HCV病毒感染密切相关,重叠感染者肝功能损害及临床失代偿程度更严重. 相似文献
55.
目的采用定点PCR技术,研究HBV的S和C基因在肝癌与癌旁组织的存在状态,探讨癌旁HBsAg表达与S基因的关系。方法福尔马林固定,石蜡包埋肝癌及癌旁组织28例,HBsAg免疫组化后的切片分别切割癌组织、癌旁HBsAg强阳性区(P2)及癌旁HBsAg阴性区(P1)各1个低倍视野,作S及C基因PCR。结果癌组织S基因较C基因检出率高(18/28vs10/28,P<0.05),癌旁二者无差异;癌旁中P1与P2的S及C基因检出率无显著性差异;高分化肝癌S和/或C基因检出率较中分化者低(2/5vs20/28,P<0.05)。结论癌组织中整合的HBVDNA为残缺不全的,HBsAg强阳性与阴性区的S基因检出无显著性差异。 相似文献
56.
Nicholas Renzette Cornelia Pokalyuk Laura Gibson Bornali Bhattacharjee Mark R. Schleiss Klaus Hamprecht Aparecida Y. Yamamoto Marisa M. Mussi-Pinhata William J. Britt Jeffrey D. Jensen Timothy F. Kowalik 《Proceedings of the National Academy of Sciences of the United States of America》2015,112(30):E4120-E4128
57.
The hepatitis B virus (HBV) capsid is an attractive drug target, relevant to combating viral hepatitis as a major public health concern. Among small molecules known to interfere with capsid assembly, the phenylpropenamides, including AT130, represent an important antiviral paradigm based on disrupting the timing of genome packaging. Here, all-atom molecular dynamics simulations of an intact AT130-bound HBV capsid reveal that the compound increases spike flexibility and improves recovery of helical secondary structure in the spike tips. Regions of the capsid-incorporated dimer that undergo correlated motion correspond to established sub-domains that pivot around the central chassis. AT130 alters patterns of correlated motion and other essential dynamics. A new conformational state of the dimer is identified, which can lead to dramatic opening of the intradimer interface and disruption of communication within the spike tip. A novel salt bridge is also discovered, which can mediate contact between the spike tip and fulcrum even in closed conformations, revealing a mechanism of direct communication across these sub-domains. Altogether, results describe a dynamical connection between the intra- and interdimer interfaces and enable mapping of allostery traversing the entire core protein dimer. 相似文献
58.
Luciana Barros de Arruda Fabrício Souza Campos Jnatas Santos Abraho Flvio Guimares da Fonseca Joo Pessoa Araújo Junior Fernando Rosado Spilki 《Viruses》2021,13(3)
The year 2020 was profoundly marked by the emergence and spread of SARS-CoV-2, causing COVID-19, which represents the greatest pandemic of the 21st century until now, and a major challenge for virologists in the scientific and medical communities. Increased numbers of SARS-CoV-2 infection all over the world imposed social and travel restrictions, including avoidance of face-to-face scientific meetings. Therefore, for the first time in history, the 2020 edition of the Brazilian Society of Virology (SBV) congress was totally online. Despite the challenge of the new format, the Brazilian society board and collaborators were successful in virtually congregating more than 921 attendees, which was the greatest SBV participant number ever reached. Seminal talks from prominent national and international researchers were presented every night, during a week, and included discussions about environmental, basic, animal, human, plant and invertebrate virology. A special roundtable debated exclusively new data and perspectives regarding COVID-19 by some of the greatest Brazilian virologists. Women scientists were very well represented in another special roundtable called “Young Women Inspiring Research”, which was one of the most viewed and commented section during the meeting, given the extraordinary quality of the presented work. Finally, SBV offered the Helio Gelli Pereira award for one graduate and one undergraduate student, which has also been a fruitful collaboration between the society and Viruses journal. The annual SBV meeting has, therefore, reached its goals to inspire young scientists, stimulate high-quality scientific discussion and to encourage global collaboration between virologists. 相似文献
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60.
Mike C. Wolf Alexander N. Freiberg Tinghu Zhang Zeynep Akyol-Ataman Andrew Grock Patrick W. Hong Jianrong Li Natalya F. Watson Angela Q. Fang Hector C. Aguilar Matteo Porotto Anna N. Honko Robert Damoiseaux John P. Miller Sara E. Woodson Steven Chantasirivisal Vanessa Fontanes Oscar A. Negrete Paul Krogstad Asim Dasgupta Anne Moscona Lisa E. Hensley Sean P. Whelan Kym F. Faull Michael R. Holbrook Michael E. Jung Benhur Lee 《Proceedings of the National Academy of Sciences of the United States of America》2010,107(7):3157-3162
We describe an antiviral small molecule, LJ001, effective against numerous enveloped viruses including Influenza A, filoviruses, poxviruses, arenaviruses, bunyaviruses, paramyxoviruses, flaviviruses, and HIV-1. In sharp contrast, the compound had no effect on the infection of nonenveloped viruses. In vitro and in vivo assays showed no overt toxicity. LJ001 specifically intercalated into viral membranes, irreversibly inactivated virions while leaving functionally intact envelope proteins, and inhibited viral entry at a step after virus binding but before virus–cell fusion. LJ001 pretreatment also prevented virus-induced mortality from Ebola and Rift Valley fever viruses. Structure–activity relationship analyses of LJ001, a rhodanine derivative, implicated both the polar and nonpolar ends of LJ001 in its antiviral activity. LJ001 specifically inhibited virus–cell but not cell–cell fusion, and further studies with lipid biosynthesis inhibitors indicated that LJ001 exploits the therapeutic window that exists between static viral membranes and biogenic cellular membranes with reparative capacity. In sum, our data reveal a class of broad-spectrum antivirals effective against enveloped viruses that target the viral lipid membrane and compromises its ability to mediate virus–cell fusion. 相似文献