用125I-M胆碱受体亚型特异抗体测定大鼠脏器M受体亚型的分布

目的人类多种疾病在Ｍ胆碱受体（Ｍ－ＡｃｈＲ）亚型的分布上有差别。本研究旨在根据Ｍ受体的氨基酸序列,合成人工多肽抗原,研究大鼠主要脏器Ｍ受体亚型的分布。方法制备特异性Ｍ１和Ｍ２受体抗体,用１２５标记两种抗体,注射到大鼠体内。结果研究显示Ｍ１和Ｍ２受体在大鼠心脏、肝脏、气管平滑肌、肾脏及肺组织中分布不同。结论人工抗体抗原诱发的Ｍ受体亚型抗体特异性好,为进行Ｍ受体亚型检测提供比较简便、可靠的方法。     

上海地区汉人瘦素受体基因G1n223Arg变异的研究

目的探讨瘦素受体基因Gln223Arg变异与肥胖的关系。方法359例无亲缘关系的上海地区汉人及66例白种人运用PCR-RFLP方法（MspI限制性内切酶）检测瘦素受体基因Gin223Arg变异的基因型。结果中国人中GG、GA及AA基因型频率分别为0.789,0.200及0.011;G及A等位基因频率分别为0.889及0.111,与本研究组中白种人的基因型及等位基因频率分布有显著差异（P=2.98×10-16,3.46×10-18）。在中国人正常体重组与超重组间该基因变异的基因型及等位基因频率分布无显著差异（P=0.369,0.344）。结论瘦素受体基因Gln223Arg变异,在中国人肥胖的发病中可能无重要作用。     

Interaction of ivermectin with γ-aminobutyric acid receptors in Trichinella spiralis muscle larvae

The value of the γ-aminobutyric acid (GABA) receptor of nematodes as a target for ivermectin's mode of action remains unclear. Using binding assays, we examined extracts from Trichinella spiralis muscle larvae for the presence of [³H]-ivermectin and [³H]-GABA binding sites. Tissue preparations displayed affinity binding sites for [³H]-ivermectin with a dissociation constant (K _d) of 83 nM and a receptor density (B_max) of 145 fmol/mg protein. We also identified a specific [³H]-GABA binding activity with a K _d of 1.2 μM and a B_max of 4.78 pmol/mg protein. In competition studies, ivermectin was found to be a competitive inhibitor of specific [³H]-GABA binding activity with an inhibition constant (K _i) of 3.39 nM, suggesting that GABA receptors could be implicated in the mechanism of action of ivermectin in nematodes. Received: 7 August 1998 / Accepted: 22 September 1998     

The inhibitory receptor superfamily: potential relevance to atopy

The role of inhibitory receptor superfamily (IRS) members in the pathophysiology of atopy is still largely unexplored but the past year or so has brought at least three important advances in the understanding of IRS member function: first, several targets of an inositol-phosphatase-dependent inhibitory signaling pathway utilized by some IRS members were identified; second, there is accumulating evidence from murine models supporting the importance of inhibitory Fc gamma receptors in modulating immune-complex-mediated inflammation; third, the inhibitory signaling capability of several previously identified candidate IRS members--including several expressed on cell types implicated in atopy and allergic reactions--has been demonstrated.     

Immunohistochemical localization of receptors for progesterone and oestradiol-17 beta in the implantation site of the rhesus monkey

The aim of the present study was to examine the cellular basis of the involvement of oestradiol and progesterone in blastocyst implantation in the primate. To this end, the cellular distribution of receptors for oestradiol (ER) and progesterone (PR) in fetal trophoblast cells and in endometrial compartments of timed lacunar (pre-villous) and villous stages of placentation in primary implantation sites collected on days 13-22 of gestation were investigated in rhesus monkeys. Both in pre-villous stage tissue and in villous stage tissue, cytotrophoblast cells and syncytiotrophoblast cells and other trophoblast derived cells were PR positive, while they were generally ER negative. Maternal endometrial cells were ER negative, while epithelial cells, stromal cells and vascular endothelial cells in maternal endometrium showed heterogeneous staining patterns for PR depending on their relative location; these patterns, however, correlated well with glandular hyperplasia and differentiation, stromal-decidual transformation and vascular response seen during blastocyst implantation.     

雌激素受体与代谢型谷氨酸受体在延髓和脊髓的分布与共存

采用免疫细胞化学双重标记方法研究了雌性大鼠延髓和脊髓内雌激素受体（ＥＲ）和代谢型谷氨酸受体１亚型（ｍＧｌｕＲ１）的分布与共存,以探讨在受体水平神经与内分泌的相互关系。结果发现：链霉亲和素ＡＢＣ法显示ＥＲ免疫反应产物呈棕色,ｍＧｌｕＲ１免疫反应产物呈蓝黑色。ＥＲ和ｍＧｌｕＲ１在脊髓和延髓有广泛的分布：在脊髓的颈、胸、腰段,ＥＲ分布于腹角、背角和侧角;ｍＧｌｕＲ１的分布与ＥＲ相似,但以腹角和背角较为丰富。在延髓,ＥＲ分布于舌下神经核、迷走神经背核、三叉神经脊束核、孤束核和网状结构;ｍＧｌｕＲ１的分布与ＥＲ相似,但在数量上有差别。此外,在延髓和脊髓ＥＲ和ｍＧｌｕＲ１广泛共存于许多神经细胞内,即存在着双标细胞。结果提示：雌激素和谷氨酸可通过共存于同一神经细胞的受体,进而在信使、基因和转录水平相互作用,调节神经细胞的机能活动     

恶性肿瘤放疗前后血清可溶性白细胞介素2受体含量变化的临床意义

目的　探讨血清可溶性白细胞介素２ 受体（ｓ Ｉ Ｌ２ Ｒ）水平与恶性肿瘤患者的病期及疗效的关系。方法　采用双抗体夹心 Ｅ Ｌ Ｉ Ｓ Ａ 法检测１５９ 例恶性肿瘤患者放疗前后血清ｓ Ｉ Ｌ２ Ｒ 水平。结果　恶性肿瘤患者放疗前血清ｓ Ｉ Ｌ２ Ｒ 水平明显高于正常对照组（ Ｐ＜ ００５）;放疗后血清ｓ Ｉ Ｌ２ Ｒ 水平明显低于放疗前（ Ｐ ＜ ０００１）;晚期患者（Ⅲ＋ Ⅳ期）不论是放疗前或放疗后 ｓ Ｉ Ｌ２ Ｒ 水平均明显高于早期患者（Ⅰ＋ Ⅱ期）（ Ｐ ＜ ００５）;各类恶性肿瘤之间血清ｓ Ｉ Ｌ２ Ｒ 水平无显著性差异（ Ｐ ＞００５）。结论　ｓ Ｉ Ｌ２ Ｒ水平在各种恶性肿瘤中的表达无特异性;检测恶性肿瘤患者ｓ Ｉ Ｌ２ Ｒ 放疗前后水平,是对病情估计和治疗疗效评价的一项参考指标。     

Kentanserin对大鼠心肌缺血 再灌注损伤的影响

为探讨５ ＨＴ２受体与心肌缺血 再灌注损伤的关系,观察５ ＨＴ２受体阻断剂ｋｅｎｔａｎｓｅｒｉｎ对心肌缺血 再灌注大鼠室颤及左室内压＋ｄｐ／ｄｔｍａｘ及－ｄｐ／ｄｔｍａｘ的影响。结果表明,ｋｅｎｔａｎｓｅｒｉｎ可拮抗缺血性心功能障碍和再灌室颤。提示５ ＨＴ２受体参与了缺血性心功能障碍和再灌室性心律失常的发生。     

表皮生长因子受体在结直肠癌的表达及临床病理意义

目的 探讨表皮生长因子受体（ＥＧＦＲ）在结直肠癌中的表达情况及其与病理特征和预后的关系。方法 采用免疫组化ＳＰ法对有随访资料的５５例结直肠癌标本进行ＥＧＦＲ检测,结合图像分析观察判定结果。结果 结直肠癌ＥＧＦＲ表达的阳性率为６９．０９％,ＥＧＦＲ的表达与肿瘤的浸润深度,淋巴结转移,Ｄｕｋｅ‘ｓ分期和生存期有关,而与分化程度无关。结论ＥＧＦＲ在结直肠癌的扩散和转移过程中可能产生一定的作用,并可作为预     

无排卵综合征大鼠肝脏、卵巢和肾上腺胰岛素受体的鉴定

目的探讨高胰岛素高雄激素无排卵综合征的发病机制。方法采用放射配体结合分析法首次对雄激素致高胰岛素高雄激素无排卵大鼠（ＡＳＲ）肝脏、卵巢和肾上腺的的胰岛素受体作了鉴定,并与正常对照大鼠进行比较。结果ＡＳＲ肝脏、卵巢和肾上腺胰岛素受体的数目及亲和力均与正常大鼠相似。结论ＡＳＲ不存在受体水平的胰岛素拮抗。