荧光光谱法分析煤焦油组分

研究煤焦油组分时,为了防止组分相互之间的干扰,在测定前用几种不同极性的溶剂:石油醚;80%石油醚-20%甲苯;60%石油醚-40%甲苯;40%石滑醚-60%甲苯;20%石油醚-80%甲苯;甲苯;氯仿;50%氟仿-50%甲醇;甲醇进行洗提,从煤焦油中得到9个级分。然后用荧光法从9个级分中鉴定出28个组分:茶、苊、联苯、三甲基酚、菲、异喹啉、芴、对氨基酚、联苯胺、蒽醌、四氢萘、2,5-二甲酚、蒽、咔唑、间苯二酚、二乙基苯胺、喹啉、吲哚醌、1,3,5-三甲苯、1,2,4-三甲苯、萘醌、菲醌、窟、苾、氧芴、1,2-苯并蒽、2,3-苯并蒽、3,4-苯并苾。同时定量了氯仿和甲醇级分中氧芴、(艹屈)、联苯胺、苾、菲、蒽、咔唑、四氢蔡咔、四氢萘、苯醌、喹啉、8-羟基喹啉等12个组分的含量。     

病原生物学多媒体网络互动教学实验室的建设与展望

介绍了病原生物学多媒体网络互动教学实验室的建设和应具备的功能 ,论述了多媒体互动网络的建成对病原生物学实验教学环境和实验教学效果的积极促进作用 ,并对实验室以后的发展方向进行了展望     

基于小波模极大原理的脉象特征提取研究

目的 根据小波分析原理，研究脉象特征提取方法，以有效解决基于时域或频域的传统方法所无法准确提取脉象特征的问题。方法 运用小波模极大方法进行脉象信号周期分割和基本分解，沿时间排列并符号化脉象波上升沿与下降沿的模极大特征点、脉象波谷底和波峰的模极大特征点。结果 确定了不明显的重搏前波、重搏波，以及在主波上升沿和下降沿上出现的不规则脉波，并进一步准确提取了脉象信号的多尺度特征和各种时域特征。结论 采用此方法进行脉象信号的特征点定位并进一步完成特征提取，具有简单、快速、准确等特点，为进一步进行脉象分类识别研究奠定了基础。     

解毒降浊、益气活瘀是治疗老年缺血性卒中的主要治则

老年缺血性卒中在病因病机、治疗方法等方面迥异于老年前期缺血性卒中患者。根据老年人的生理病理特点结合临床实际，认为正虚血瘀为本，浊毒内蕴为标，毒损脑络为老年脑缺血损伤的终结；正虚血瘀、浊毒内蕴是老年脑缺血损伤的根本原因；解毒降浊、益气活血是治疗老年缺血性卒中的重要方法之一。     

克痛喷剂的质量标准研究

采用薄层色谱法对克痛喷剂中蟾酥、冰片、大黄、栀子、白芷进行定性鉴别；并应用气相色谱法对冰片的含量进行测定。实验结果证明方法简便，斑点圆整，重现性及专属性均好，可国该制剂的质量控制标准。     

用三参量模糊分析法识别潜水减压气泡信号的研究

目的 探讨潜水减压多普勒超声气泡信号的模糊识别方法。方法 根据气泡信号的频谱分布特征，构建f-f-△p三参量模糊算法，并通过减压病动物模型进行验证，同时对66例氦氧150m饱和-180m巡回潜水减压的数据进行检测。结果 在减压病动物模型中分别检测到I～Ⅱ级气泡(按Spencer分级标准)，气泡数量6～113个／3s内不等；在饱和潜水减压资料中，检测到1人两次有I级气泡音，气泡数量分别为3个(11s录音)与6个(17s录音)，与人工监听结果基本一致。结论 用三参量模糊分析方法充分借鉴了多年来人工分析所积累的经验，同时利用了计算机辅助分析技术，气泡信号的检测分析较为准确客观。     

Proteolytic artifacts in SDS-PAGE analysis of selected periodontal pathogen:

The aim of the study was to examine whether proteolytic artifacts, which result in a loss and poor resolution of protein bands, occur during sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis analysis of cellular proteins from selected proteolytic ( Porphyromonas gingivalis, Prevotella nigrescens and Treponema denticola ) and non-proteolytic ( Fusobacterium nudeatum ) bacteria. Conditions to limit or prevent proteolysis were also investigated. Bacterial cells were incubated in solubilizing buffer (SDS +β mercaptoethanol) at room temperature for various periods of time before boiling. A control assay consisted of trichloroacetic acid-treated bacterial cells. Cellular proteins were separated by electrophoresis and stained with Coomassie blue. Proteolysis occurred very rapidly in the case of P. gingivalis (<30 s), whereas a longer incubation time (>1 h) was required to observe similar effects in P. nigrescens and T. denticola. No proteolysis was observed for F. nudeatum. In all cases, heat (100°C) and low pH (<4) treatments of bacterial cells could avoid production of proteolytic artifacts. Incorporation of specific protease inhibitors before solubilization of bacteria could also prevent proteolysis. More particularly, N -α- p -tosyl- l -lysine chloromethyl ketone (TLCK), iodoacetamide and diisopropylfluorophosphate (50 mM) were highly efficient for P. gingivalis, P. nigrescens and T. denticola , respectively. When outer membranes of P. gingivalis were prepared in the presence of TLCK, numerous additional protein bands, not seen in the absence of TLCK, were detected. The present study suggests that specific protease inhibitors, effective in preventing proteolysis. should be identified and added during cell fractionation and protein purification procedures.     

Techniques in facial identification: Computer-aided facial reconstruction using a laser scanner and video superimposition

A facial image was reconstructed from the skull, part of a complete skeleton found in woodland, of a male person who had hanged himself from a tree. In addition, video superimposition was carried out with antemortem photographs of a person suspected of being the victim, and a good match was obtained. In a further case, a cheaper video-transparency superimposition was carried out, with identity later being confirmed on the basis of dental records. The techniques and the problems encountered are discussed. According to our experience, 3D computer reconstruction and video superimposition have a useful role in the process of identification, particularly in the early stages of an investigation and when other more definitive methods may not be available.     

Development of a real-time fluorescence resonance energy transfer PCR to identify the main pathogenic Campylobacter spp.

A simple real-time fluorescence resonance energy transfer (FRET) PCR, targeting the gyrA gene outside the quinolone resistance-determining region, was developed to identify Campylobacter jejuni and Campylobacter coli. These species were distinguished easily, as the corresponding melting points showed a difference of 15 degrees C. A second assay using the same biprobe and PCR conditions, but different PCR primers, was also developed to identify the less frequently encountered Campylobacter fetus. These assays were applied to 807 Campylobacter isolates from clinical specimens. Compared to phenotypic identification tests, the FRET assay yielded the same results for all except three of the isolates. Analysis by standard PCR and 16S rDNA sequencing demonstrated that two of these isolates were hippurate-negative C. jejuni strains, resulting in an erroneous phenotypic identification, while the third was an isolate of C. coli that contained a gyrA gene typical of C. jejuni, resulting in misidentification by the FRET assay. The FRET assay identified more isolates than standard PCR, which failed to yield amplification products with c. 10% of isolates. It was concluded that the FRET assays were rapid, reliable, reproducible and relatively cost-efficient, as they require only one biprobe and can be performed directly on boiled isolates.