HISTOMICROBIOLOGIC ASPECTS OF THE ROOT CANAL SYSTEM AND PERIAPICAL LESIONS IN DOGS' TEETH AFTER ROTARY INSTRUMENTATION AND INTRACANAL DRESSING WITH Ca(OH)2 PASTES

OBJECTIVE:

The purpose of this study was to evaluate the distribution of microorganisms in the root canal system (RCS) and periapical lesions of dogs'' teeth after rotary instrumentation and placement of different calcium hydroxide [Ca(OHy-based intracanal dressings.

MATERIALS AND METHODS:

Chronic periapical lesions were experimentally induced in 80 premolar roots of four dogs. Instrumentation was undertaken using the ProFile rotary system and irrigation with 5.25% sodium hypochlorite. The following Ca(OH)₂-based pastes were applied for 21 days: group 1 - Calen (n=18); group 2 - Calen+CPMC (n=20); group 3 - Ca(OH₂ p.a. + anaesthetic solution (n=16) and group 4 - Ca(OH₂ p.a.+ 2% chlorhexidine digluconate (n=18). Eight root canals without endodontic treatment constituted the control group. Histological sections were obtained and stained with Brown & Brenn staining technique to evaluate the presence of microorganisms in the main root canal, ramifications of the apical delta and secondary canals, apical cementoplasts, dentinal tubules, areas of cemental resorption and periapical lesions. The results were analyzed statistically by the Mann-Whitney U test (p<0.05).

RESULTS:

The control group showed the highest prevalence of microorganisms in all sites evaluated. Gram-positive cocci, bacilli and filaments were the most frequent morphotypes. Similar microbial distribution patterns in the RCS and areas of cementum resorption were observed in all groups (p>0.05). The percentage of RCS sites containing microorganisms in groups 1, 2, 3, 4 and control were: 67.6%, 62.5%, 78.2%, 62.0% and 87.6%, respectively.

CONCLUSION:

In conclusion, the histomicrobiological analysis showed that the rotary instrumentation and the different calcium hydroxide pastes employed did not effectively eliminate the infection from the RCS and periapical lesions. However, several bacteria seen in the histological sections were probably dead or were inactivated by the biomechanical preparation and calcium hydroxide-based intracanal dressing.     

辨证配合脐疗治疗慢性荨麻疹100例临床研究

目的研究以补气和血汤为主辨证配合脐疗治疗慢性荨麻疹的临床疗效。方法应用以补气和血汤为主辨证配合脐部(神阙穴)拨火罐,再用加味玉屏风散适量醋调敷脐,对100例慢性荨麻疹患者进行临床治疗观察;对照组口服防风通圣丸,两组均治疗1周为一个疗程,连续4个疗程后进行疗效评估。结果治疗组与对照组痊愈率分别为70.00%和19.00%,差异有极显著性(P<0.005)。结论以补气和血汤为主辨证配合脐疗治疗慢性荨麻疹有较好的疗效。     

隔药灸脐治疗脾肾阳虚型慢性疲劳综合征的临床观察

[目的]观察隔药灸脐治疗脾肾阳虚型慢性疲劳综合征的临床疗效,为临床治疗慢性疲劳综合征提供方法和思路。[方法]选取2018年1月至2019年2月就诊我院的脾肾阳虚型慢性疲劳综合征患者90例,随机分为观察组与对照组各45例。对照组予以普通针刺治疗,观察组在对照组基础上予隔药灸脐治疗。对比分析两组患者治疗前后主要症状(记忆力减退、失眠抑郁、紧张焦虑)评分、疲劳严重度量表(fatigue severity scale,FSS)、健康调查简表(the MOS item short from health survey,SF-36)评分,并比较临床疗效。[结果] 治疗后,观察组临床疗效明显优于对照组,差异具有统计学意义(P＜0.05)。两组治疗后的主要症状评分、FSS评分、SF-36评分均明显优于治疗前,差异具有统计学意义(P＜0.05);治疗后观察组主要症状评分、FSS评分、SF-36评分均明显优于对照组,差异具有统计学意义(P＜0.05)。[结论] 隔药灸脐联合普通针刺治疗脾肾阳虚型慢性疲劳综合征,疗效优于单纯常规针刺治疗,说明隔药灸脐治疗在改善疲劳症状、提高生活质量方面具有一定优势。