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101.
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103.
Indications of immunological tolerance in kidney transplantation 总被引:5,自引:0,他引:5
104.
Polymorphic variants in the gene encoding factor VII (F7) affect the plasma levels of this coagulation protein and modify the clinical phenotype of FVII deficiency in some patients. In this study we report the in vitro functional analysis of a novel polymorphic variant located in the 3' untranslated region of F7: g.11293_11294insAA. To determine whether this variant regulates FVII expression, we initially compared an expression vector containing FVII cDNA with g.11293_11294insAA with the FVII wild-type (WT) construct. The kinetics of mRNA production showed that the insertion decreases the steady-state FVII mRNA levels. To assess whether the insertion influences the phenotype of FVII-deficient patients, we evaluated its effect on the expression of FVII in a patient with severe FVII deficiency (undetectable FVII activity and antigen) carrying two additional homozygous missense variations (p.Arg277Cys and p.Arg353Gln). The two substitutions alone reduced the expression of FVII activity and antigen in vitro, but with the insertion polymorphism in our expression vector the patient's phenotype of undetectable plasma FVII was recapitulated. The insertion polymorphism in the 3' untranslated region of F7 is another modifier of FVII expression that might explain the poor genotype-phenotype correlation in some FVII-deficient patients. 相似文献
105.
A. Seto-Ohshima P. C. Emson M. W. Berchtold C. W. Heizmann 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1989,75(3):653-658
Summary Parvalbumin mRNA was localized in rat brain by in situ hybridization using a 35S labelled rat parvalbumin cDNA and a synthetic oligodeoxyribonucleotide (corresponding to base sequences 140 to 183 of rat parvalbumin cDNA). Strongest hybridization signals were detected in the Purkinje cells of the cerebellum and in neurones of the reticular nucleus of the thalamus. Signal was also detected in the cerebral cortex, hippocampus, basal ganglia and brain stem in agreement with the distribution of parvalbumin immunoreactivity. 相似文献
106.
107.
Yasuhiro Yoshikawa Hiroshi Tsuruoka Mineo Matsumoto Takeshi Haga Tatsuo Shioda Hiroshi Shibuta Takeshi A. Sato Kazuya Yamanouchi 《Virus genes》1990,4(2):151-161
The nucleotide sequence of a cloned cDNA corresponding to the P+M dicistronic mRNA of a subacute sclerosing panencephalitis (SSPE) virus was determined and compared with data of measles virus (MV). The dicistronic mRNA of the SSPE virus consisted of the 3 proximal 626 nucleotides of P mRNA, intercistronic trinucleotides, a full length of M mRNA, and 75 poly A nucleotides. The part encoding the P protein had a high homology to MV, except at the noncoding region. The terminating consensus sequence of the P gene and the intercistronic trinucleotides of the SSPE virus were CTAC(A)6 and CCT; in MV they are TTAT(A)6 and CTT, respectively. In the M gene, the starting consensus sequence was exactly the same as MV, but at the 5 proximal end, one third of this gene was different: The first ATG codon of the MV M gene signaling opening of the reading frame was changed to ACG in the SSPE virus and one long open reading frame started from the third ATG codon. The stop codon (TAG) of the MV M gene was also changed to CAG in the SSPE virus. Thus, the deduced SSPE-virus M protein lacked 50 amino acids at the amino terminal and had 15 extra amino acids at the carboxyl end when compared with the MV M protein. 相似文献
108.
目的:探讨KLK6蛋白及其mRNA在原发性乳腺癌组织中的表达和临床意义.方法:随机选取原发性乳腺癌患者88例并收集其术后标本, 采用SABC免疫组化方法和RT-PCR技术, 检测乳腺癌组织和正常乳腺组织中KLK6蛋白及其mRNA的表达.并分析其与原发性乳腺癌组织临床病理学特征之间的关系.结果:KLK6蛋白在原发性乳腺癌组织中的阳性表达率为78.40% (69/88), 并与癌组织的临床病理学特征有关;发生淋巴结转移及ER( )的癌组织中KLK6蛋白的阳性表达率明显低于未转移组(P<0.05)及ER(-)组(P<0.05).KLK6 mRNA在原发性乳腺癌组织中的表达水平显著高于正常乳腺组织(P<0.01);但发生淋巴结转移的癌组织中, KLK6 mRNA的表达水平明显低于未转移组(P<0.05);ER 组亦低于ER(-)组(P<0.05).KLK6蛋白及其mRNA的表达与乳腺癌相关基因CerbB-2 的表达无相关性(P>0.05) .结论:KLK6蛋白及其mRNA的异常表达可能与原发性乳腺癌的发生、浸润、转移有关, 可以作为一个肿瘤标志物在临床中应用. 相似文献
109.
Norio Yoshimura Takahiro Oka Masakazu Kita Hiroshi Teraoka Yoshikatsu Hirai 《Journal of clinical immunology》1989,9(4):322-328
The present study examined the effect of cyclosporine (CsA) administered with steroidin vivo on the capacity of peripheral blood mononuclear cells (PBMC) from kidney transplant recipients to generate cytokines and their gene expression at the level of messenger RNA (mRNA). PBMC from CsA-prednisolone (Pred)-treated recipients displayed 66.9% inhibition (54.3±12.4 IU/ml;N=42;P<0.01) of -interferon (-IFN) production compared with normal individuals (134.6±18.6 IU/ml;N=23). Azathioprine (Az)-Pred-treated recipients displayed significantly less inhibition of -IFN generation (96.0±16.1 IU/ml;N=22;P<0.05) than CsA-treated patients. Macrophages (m) from CsA-Pred-treated recipients displayed 60.0% inhibition (5.1±0.7 U/ml;N=20;P<0.01) of interleukin-1 (IL-1) production compared with normal individuals (13.0±2.9 U/ml;N=21). These results were confirmed by the experiments using cDNA probe for -IFN or IL-1 (, ). High levels of -IFN mRNA in phytohemagglutinin (PHA)-stimulated PBMC or IL-1() mRNA in lipopolysaccharide (LPS)-stimulated m were present in normal individuals but not in CsA-treated recipients as judged by hybridization to a cloned human -IFN or IL-1() cDNA probe. These studies demonstrated that combination therapy of CsA with steroid inhibits both -IFN and IL-1 gene expression at the level of mRNA at physiological concentrations. 相似文献
110.
Isolation and characterization of a cDNA coding for a novel human 17.3K myelin basic protein (MBP) variant 总被引:12,自引:0,他引:12
H J Roth K Kronquist P J Pretorius B F Crandall A T Campagnoni 《Journal of neuroscience research》1986,16(1):227-238
Human fetal spinal cord poly A (+) mRNA was found to direct the synthesis of three major myelin basic protein (MBP) variants with molecular weights of 17K, 18.5K, and 21.5K when translated in reticulocyte lysates. In order to investigate the structural relationships between these MBP variants and their corresponding mouse variants, human fetal spinal cord and mouse brain cDNA libraries were constructed and screened for MBP cDNAs. A number of MBP cDNA clones were isolated and characterized. One of these, PP535 contained the entire coding region of the mouse 14K MBP; and another mouse cDNA clone, PP1.85, was almost full-length and coded for either the 21.5K MBP or the 18.5K MBP. A human clone (KK36), 1,173 nucleotides in length, contained the entire coding region of an MBP variant with a molecular weight of 17,342. The structure of this clone within its coding region is significantly different from the corresponding mouse 17K MBP cDNA. It is missing two sequences found in the mouse 17K MBP cDNA (exons 2 and 5); and it contains a sequence (exon 6) that is missing from the mouse 17K MBP cDNA. Thus, this human 17.3K cDNA codes for a "17K" human MBP variant that is quite different from the corresponding mouse variant and is identical to the human 18.5K MBP except for a deletion of a peptide consisting of 11 amino acids that includes the single tryptophan residue of the 18.5K MBP. An analysis of the structure of this 17.3K human MBP cDNA suggests that the major pathway for splicing the primary human MBP gene product may be different from that in the mouse. 相似文献