Highly sensitive simultaneous electrochemical determination of myricetin and rutin via solid phase extraction on a ternary Pt@r-GO@MWCNTs nanocomposite

The simultaneous electrochemical determination of myricetin and rutin remains a challenge due to their indistinguishable potentials. To solve this problem, we constructed a ternary platinum nanoparticle, reduced graphene oxide, multi-walled carbon nanotubes (Pt@r-GO@MWCNTs) nanocomposite via a facile one-pot synthetic method. Under the optimized conditions, the ternary Pt@r-GO@MWCNTs nanocomposite exhibited good electrocatalytic activity toward myricetin and rutin via solid phase extraction and excellent performance for the simultaneous determination of myricetin and rutin. The oxidation peak current of myricetin was proportional to its concentrations in the range of 0.05–50 μM with a detection limit of 0.01 μM (S/N = 3). The linear range for rutin was 0.05–50 μM with a detection limit of 0.005 μM (S/N = 3). The ternary nanocomposite sensor also exhibited good reproducibility and stability, and was successfully used for the simultaneous determination of myricetin and rutin in real orange juice samples with recoveries ranging between 100.57% and 108.46%.     

高效液相色谱法测定仙蓟化斑胶囊中芦丁的含量

目的:建立高效液相色谱法对仙蓟化斑胶囊中芦丁含量的测定方法。方法:采用YWG—C18反相柱(4.6mm×200mm,5um)色谱柱,流动相:甲醇-水-冰醋酸(40:58:3);进样量10ul;室温;流速1ml/min;检测波长为360nm。结果:芦丁对照品在0.105~0.525μg之间,进样量与峰面积间呈良好的线性关系,r=0.9998,平均回收率为101.6%;RSD为1.52%。结论:该法快速、准确,操作简便,可用于本产品的质量控制。     

新疆产两种槐花中芦丁和槲皮素的含量测定

目的：采用反相高效液相色谱法（RP－HPLC）测定了新疆产两种槐花（槐、刺槐）中芦丁及槲皮素含量。方法：色谱柱：ZorbaxC18柱,流动相：甲醇－水－乙酸（40：57．5：2．5）,检测波长：254nm,流速1．0ml/min。结果：槐和刺槐花蕾、花中芦丁的含量分别为13．88％、10．48％、0．1288％、0．1744％,槐花花蕾、花中槲皮素的含量为0．1415％、0．2158％。结认：为地产槐花的开发利用提供了依据。     

均质法提取侧柏叶中黄酮类化合物的研究

目的选择均质法从侧柏叶中提取黄酮类化合物。方法采用比色法,以芦丁为对照品,建立了含量测定法。采用单因素试验法,对比超声、微波、回流和均质提取法,并利用正交试验,以均质时间、甲醇浓度、料液比和次数4个因素进行考察,结果总黄酮浓度在0.008 96~0.053 76 mg/m L范围内呈良好的线性关系,加样回收率为102.07,RSD为2.77%。正交试验结果:均质时间15 min,甲醇浓度为70%,料液比1∶10,提取1次。结论所建立的含量测定法简单易行,均质法比其他方法更为简便,省去对物料粉碎的步骤,且正交试验确定的试验结果稳定、可靠,可用于工业生产。     

HPLC法同时测定杏花中阿魏酸、芦丁和异槲皮苷

目的建立HPLC法同时测定杏花中阿魏酸、芦丁和异槲皮苷。方法色谱柱为YMC-Pack ODS-A C_(18)柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸溶液(15∶85);体积流量1.0 mL/min;检测波长为343 nm;柱温为30℃。结果阿魏酸、芦丁和异槲皮苷分别在0.029 08~0.290 8μg(r=0.999 9)、0.548 4~5.484 2μg(r=0.999 9)、0.033 07~0.330 7μg(r=0.999 9)范围内线性关系良好;平均回收率分别为98.31%、98.93%、100.25%,RSD分别为1.0%、0.9%、1.1%。10批杏花样品中阿魏酸、芦丁、异槲皮苷含量测定结果分别为0.105~0.554、3.080~6.933、0.258~0.974 mg/g。结论该方法多种成分同时测定,操作简便、准确,重复性好,可用于杏花药材的质量控制。     

Preparation and Characterization of Rutin-Encapsulated Polymeric Micelles and Studies of Synergism with Bioactive Benzoic Acids and Triazolofluoroquinolones as Anticancer Nanomedicines

Background: The study aimed to examine rutin micelles of advanced superlative dual cytotoxicity-antiinflammtion bioefficacies in substantially novel submicro-nanoaffinities vs. both the raw rutin and reference proapoptotic cisplatin. Methodology: Antiproliferative capabilities of rutin, benzoic acid (BA) and triazolofluoroqunolone (TFQ) derivatives were reported; hence chemosensitizing effects of rutin or its polymeric micelles (of improved solubility and bioavailability via direct dissolution using the amphiphilic copolymer Pluronic P123) in co-incubations with 5 BAs or 3 TFQ derivatives in a panel of 6 cancer cell lines were verified. Results: Rutin loading in micelles was achieved with a loading efficiency of 59.5 ± 2.9%. The particle size of the micelles was found to be 18 ± 2 nm. Though Rutin loaded nanomicelles were of minimal DPPH radical scavenging activity; they had nitrogen oxide (NO) radical scavenging activity in lipopolysaccharide-induced RAW264.7 macrophages with equipotency to indomethacin (IC50 values (µM) 73.03 vs. 60.88; p=0.057). Remarkably nano-micelle formulation of rutin was proved of significantly more potent antineoplastic bioactivity with submicro-nanomolar affinities in the 6 cancer cell lines vs. both free rutin’s and cisplatin’s (except A549 lung cancer cell line). Rrutin nanomicelles chemo-sensitized all selected 8 cotreatments with BA derivatives and TFQs and, thus reducing the dose used against breast cancer MCF7 cells to submicro-nanomolar affinities of greater potencies than cisplatin’s. Except for Triazolo-4-anisidine cipro butyl acid in PANC1, 2-Amino-3,5-Di iodo BA in A375 and 4-Nitrophenol in A549 incubations; rutin loaded nanomicelles chemosensitized 7/8 cotreating selected benzoic acid (BAs) derivatives and TFQs and chemosensitized pancreatic PANC1, skin A375 and lung A549 cancer cell lines, thus reducing the dose to submicro-nanomolar affinities of greater potencies than cisplatin’s. Rutin loaded nanomicelles chemosensitize 6/8 cotreating selected benzoic acid (BA) derivatives and TFQs (except for 2-Amino-5-Bromo Benzoic Acid and Triazolo-4-anisidine cipro butyl acid), thus reducing the dose used against resistant CACO2 colorectal cancer cells.     

枸骨中芦丁和槲皮素含量测定

目的：采用高效液相色谱法测定枸骨不同部位（嫩叶、老叶、枝、青果、红果）及不同采收期枸骨叶中芦丁和槲皮素的含量。方法采用C18色谱柱（250 mm ×4．6 mm ,5μm）,流动相为甲醇－0．5％磷酸水溶液（47∶53）,流速为0．7 ml／min ,检测波长为356 nm ,柱温25℃,测定枸骨中芦丁和槲皮素含量。结果芦丁和槲皮素线性范围分别为0．52～5．2μg和0．34～3．4μg,相关系数 r均为0．9999,平均回收率分别为98．6％和99．2％,RSD分别为1．70％和1．91％（n＝6）。结论枸骨不同部位及不同采收期枸骨叶中芦丁和槲皮素含量有明显差异。     

槐米提取物的制备工艺及其紫外吸收特性考察

目的：优选槐米防晒成分芦丁的最佳提取工艺。方法采用紫外分光光度法检测槐米提取液在波长200~400 nm各区间的平均吸收率,采用高效液相色谱法测定其主要成分芦丁含量。分别以槐米提取液紫外吸收率、芦丁含量为指标,对超声波提取工艺进行单因素考察,并采用L9(34)正交试验优选槐米芦丁的提取工艺。结果槐米提取液在UVA、UVB、UVC波段内对紫外线均有较强的吸收作用。槐米提取液的最佳提取工艺：乙醇浓度为70%,料液比为1：30,超声时间为30 min,超声温度为65℃。结论槐米提取液在紫外各区间均有较强吸光能力,具有很好的广谱防晒能力。超声波提取法具有时间短、提取率高等优点,可用于槐米防晒成分芦丁的提取。     

一测多评法同时测定舒血宁注射液中6种黄酮类成分

摘 要 目的： 采用一测多评(quantitative analysis of multi components by single marker,QAMS)法,同时测定舒血宁注射液中6种黄酮类成分,并考察其方法的耐用性与系统适应性。方法: 以舒血宁注射液为研究对象,以芦丁为内参物,建立芦丁与水仙苷、槲皮素-3-O-β-D-葡萄糖基(1-2)-α-L-鼠李糖苷、山奈酚-3-O-β-D-葡萄糖基(1-2)-α-L-鼠李糖苷、槲皮素-3-O-[2-O-(1-O)-对香豆酰基-β-D-葡萄糖基]-α-L-鼠李糖苷、山奈酚-3-O-[2-O-(6-O)-对香豆酰基-β-D-葡萄糖基]-α-L-鼠李糖苷间的相对校正因子（f）,比较该方法与外标法测定结果的差异,并对该方法进行再验证。结果: 利用相对校正因子计算舒血宁注射液中这6种黄酮类成分的含量,与外标法比较,差异较小(RSD<5%) 。结论:QAMS 可以作为一种简便准确的质量评价模式用于舒血宁注射液多种成分的定量测定,为后续质量标准评价研究提供了参考依据。     

Determination of antioxidant activity,rutin, quercetin,phenolic acids and trace elements in tea infusions: Influence of citric acid addition on extraction of metals

Antioxidant activity of tea, content of bioactive compounds (rutin, quercetin, phenolic acids including: gallic, chlorogenic, protocatechuic, p-coumaric, caffeic, ferrulic, syringic and sinapic as well as other selected organic acids) and trace elements (Mn, selected as a metal playing a role in oxidative metabolism; Al; Cd; and Pb – the so-called heavy or noxious metals) were studied as these parameters greatly influence the quality of tea infusions. A number of different samples were tested including white, green, black and lemon-flavored teas, as well as the Red Lapacho tea. Antioxidant activity was measured using DPPH and ABTS tests, and a strong correlation between obtained results was observed. Infusions made from lemon teas packed in bags showed increased levels of rutin, quercetin and phenolic acids. Nevertheless, the positive effect of the increased level of phenolic compounds extracted is also connected with higher level of extracted noxious elements. Citric acid added to tea and herbal Red Lapacho infusions significantly increased Al, Cd, and Pb trace elements concentrations. Nevertheless, the infusions prepared from the lemon tea bags contained even the 10–70-fold higher content of these elements. On the other hand, addition of citric acid to tea infusions increased amount of extracted Mn only in black teas and Red Lapacho.