Changes induced by corticosterone and adrenalectomy in synaptosomal and platelet uptake and binding of 5-HT: The relationship to [3H]imipramine binding

A comparative study of the effect of adrenalectomy, treatment with corticosterone, and imipramine on platelet and synaptosomal uptake was undertaken. One day after adrenalectomy, uptake of 5-hydroxytryptamine (5-HT) in the platelet and the hypothalamus was significantly decreased with an increase in the apparent K_m, but the uptake of 5-HT of the cerebral synaptosomes was unchanged. In this group, the k_d) of low affinity binding of 5-HT was also increased in the hypothalamic synaptosomes and in the platelet preparations. Treatment with cortieosterone (5 $\text{mg}\text{kg}$, i.m.) restored the decrease in the uptake of 5-HT induced by adrenalectomy in the hypothalamic synaptosomes and in the platelets, and significantly increased the uptake of 5-HT of these fractions in sham-operated rats. The binding of 5-HT was unchanged by acute treatment with corticosterone. The effectiveness of imipramine varied with the preparation and treatment group. The IC₅₀ of the cerebral synaptosomal preparation was greater than that of the hypothalamic synaptosomal preparation. Using this latter preparation, the IC₅₀ for imipramine in adrenalectomised, sham-operated and corticosterone-treated rats were found to be 0.04, 0.09 and 0.25 μM, respectively. These changes in sensitivity to imipramine were not reflected in the binding of [³H]imipramine which was unchanged.     

李时珍对人体生命的认识

在与我国古代道家、医家比较中 ,探讨李时珍对人体生命的认识 ,认为李时珍在前人认识的基础上首次明确提出“脑为元神之府”、经脉为“内景隧道”、命门在“两肾之间”、“命门为体 ,三焦为用”等富有原创性学说 ,这些学说的提出均在同时代医家之前。此外李时珍还提出“脾乃元气之母”观点 ,虽非首创 ,却也是对前人的充实和发展。     

李杲"阴火说"探微

关于“阴火”理论历来有不同解释,经反复分析原文,认为“阴火”是指下焦离位之邪火,乃为病理之火,与元气不两立,又称“心火”。对“阴火”诊治之法,认为病因是脾胃元气,病机是气火失调,治疗特点是甘温益气、升阳泻火。     

“虚里”含义再识

文章认为虚里不是心尖搏动处,而是胃之大络在体内的起点。胃之大络在体表的终点——-“左乳下”才是真正的心尖搏动处。结合《内经》原文从4个方面进行论述:虚有空虚不实之意;里有处所、部位之意。虚里即体内胃部空虚无穴的部位。胃之大络在体内的起点虚设为“虚里”.“左乳下”是胃之大络在体表的终点。原文中的两个“其”字,通常多认为是指“虚里”而言,而文章认为两个“其”字都是指“左乳下”而言。长期以来,人们误将“虚里”当作“胃之大络”的代名词。把“虚里”解释成“胃之大络”.文章认为“虚里”是“点”概念,而“胃之大络”是“线”的概念,“虚里”并不是“胃之大络”的代名词。     

兼收并蓄独树一帜——吴师机内病外治法的特点及其渊源

清代名家吴师机《理瀹骈文》为外治专著,其内病外治的特点是：阐论外治机理强调整体辩证,分析辨治方法注重三焦分治,其用方经验将内服有效之汤丸散等熬制成外治之膏药,对选用药味喜用味厚力大之品。并介绍外治法的学术渊源和历史沿革。     

《理虚元鉴》学术特色探析

《理虚元鉴》是明末医家汪绮石论治虚劳的专著,该书具有鲜明的学术特色。在病因上重视情志因素,在病机上倡虚火、伏火致病学说,治疗上依三本二统,尤其重培补脾胃,重视预防,强调虚劳当治其未成,该书对临床慢性虚损性疾病辨治颇具指导意义。     

"阴火"考释

"阴火"一词屡见于李杲之前的文学作品,并与李杲的"阴火"概念密切相关,对此加以考释,对于进一步理清李杲的"阴火"概念有十分积极的意义。     

李中梓治疗泄泻学术思想探析

李中梓将湿邪作为泄泻的主要病因,并从理论和实践上进一步加以深化和完善,阐明了泄泻、湿邪、脾土三者之间的辨证关系.治疗上力主辨证求因、审因论治,总结出渗利、升提、清凉、疏利、甘缓、酸收、燥脾、温肾、固涩等治泻九法.     

拟缺香茶菜的化学成分研究

目的研究拟缺香茶菜Rabdosia excisoides的化学成分。方法利用硅胶柱色谱法进行分离和纯化,通过谱学分析鉴定单体化合物结构。结果从拟缺香茶菜分离鉴定2个化合物,化合物Ⅰ命名为拟缺香茶菜甲素(excisoidesinA);化合物Ⅱ鉴定为齐墩果酸。结论化合物Ⅰ为首次发现的新二萜成分。     

温热联合细胞周期特异性药物对恶性胸膜间皮瘤细胞的影响

通过体外实验研究温热联合细胞周期特异性药物羟基喜树碱（ＯＰＴ）对ＳＭＣ１ 恶性胸膜间皮瘤细胞株的影响。结果显示：经０１ μｇ／ｍ ｌ、１０ μｇ／ｍ ｌ、１００ μｇ／ｍ ｌＯＰＴ 处理４８ ｈ 对ＳＭＣ１ 细胞增殖有抑制作用,其抑制作用具有药物浓度依赖性,流式细胞仪测定显示经ＯＰＴ处理后细胞周期出现改变,Ｓ期细胞下降,Ｇ２＋ Ｍ 期细胞增加（Ｐ＜ ００５ 或Ｐ＜ ００１）;单独加温３９℃、４１℃、４３℃６０ ｍ ｉｎ 对ＳＭＣ１ 细胞增殖同样具有抑制作用,并有温度依赖性;温热联合ＯＰＴ 对ＳＭＣ１ 细胞增殖具有最佳抑制效果,该效果显现出温度药物浓度依赖性。该实验为临床应用提供了一定的理论依据