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51.
目的总结逆行半比目鱼肌带蒂肌瓣修复小腿远端软组织缺损的临床应用结果。方法 2006年10月~2012年10月,应用逆行半比目鱼肌带蒂肌瓣修复12例小腿远端软组织缺损,年龄16~42岁(平均35岁)。肌瓣表面行一期中厚网状游离植皮,供区直接缝合。结果所有的肌瓣全部成活,取得了满意的临床效果。随访1.5~3.5年,平均2.5年,8例伴有胫骨骨折者骨折全部愈合。结论逆行半比目鱼肌带蒂肌瓣具有血管解剖恒定、血运丰富以及操作简单等优点,适宜修复小腿远端软组织缺损。 相似文献
52.
Interstitial Stress and Fluid Pressure Within a Growing Tumor 总被引:1,自引:0,他引:1
A solid tumor is composed of a population of cells that is expanding as a result of cell division. With dense cell packing, the solid matrix of the interstitial tissue is subject to residual stress. In addition, elevated interstitial fluid pressure (IFP) has been reported by researchers for a number of solid tumors. These features were incorporated into a mathematical model that predicts the mechanical response of a solid tumor within its host environment. A theoretical framework accounting for volumetric expansion, transvascular exchange and extravascular transport of fluids was developed using poroelastic theory, and applied to a spherical, vascularized, alymphatic tumor undergoing small growth increments. Simulations of tumor IFP were similar to those predicted by Jain and Baxter (Ref. 23), showing elevated IFP that is driven by microvascular fluid pressure. Tumor growth, tissue stiffness, and IFP contribute to the compressive stresses predicted in the solid tumor interior. Tensile and compressive stresses were predicted in adjacent host tissues corresponding to radial and circumferential directions, respectively. An application of this model includes a solid stress-based framework for predicting regions of vascular collapse within the tumor interior. © 2003 Biomedical Engineering Society.
PAC2003: 8717Ee, 8710+e, 8719Rr, 8719Xx 相似文献
53.
Malignant fibrous histiocytoma: an ultrastructural perspective 总被引:1,自引:0,他引:1
Malignant fibrous histiocytoma is a frequent diagnosis, but the relationship of the tumors to histologically similar soft tissue neoplasms is controversial. In this study, 157 examples representing the 4 main subtypes were reviewed by light microscopy and each tumor was studied with the electron microscope. Immunohistochemical stains were performed on 77 tumors. Electron micrographs on 100 fibrosarcomas were reviewed for comparison. Malignant fibrous histiocytomas often closely resemble fibrosarcomas at the ultrastructural level and differences between the two are generally of degree only. Evidence for true histiocytic differentiation was not found. The immunohistochemical results did not contradict the authors' impression from electron microscopy that malignant fibrous histiocytoma forms part of the histologic spectrum of tumors of fibroblasts. 相似文献
54.
伴有肌样/肌纤维母细胞性分化的隆突性皮纤维肉瘤的临床病理分析 总被引:5,自引:0,他引:5
目的 探讨隆突性皮纤维肉瘤(DFSP)中肌样/肌纤维母细胞性分化的本质及其临床病理学意义。方法 采用常规HE切片对124例DFSP进行筛选,对6例伴有肌样/肌纤维母细胞性分化的DFSP病例进行免疫组织化学标记,其中2例加做电镜检测。结果 肌样/肌纤维母细胞性分化多出现在纤维肉瘤型DFSP(FS-DFSP)中,表现为肿瘤周边部或肿瘤内散在性分布的深嗜伊红色小结节或短要束,由梭形细胞组成,细胞多无异型性,核分裂象也罕见,形态上似平滑肌细胞或肌纤维母细胞。免疫组织化学标记显示肌样区域细胞表达α-平滑肌肌动蛋白和肌物质特异性肌动抗原,不表达CD34;电镜观察证实细胞含有质膜下微丝束、局灶性致密体及微胸饮囊泡样结构,与肌纤维母细胞相一致,结论 DFSP中的肌样/肌纤维母细胞性分化可能是间质中肌纤维母细胞增生的结果,并非代表了瘤细胞的真性肌纤维母细胞性分化。 相似文献
55.
Kawaguchi K Oda Y Saito T Takahira T Yamamoto H Tamiya S Iwamoto Y Tsuneyoshi M 《Human pathology》2005,36(4):357-363
The PTEN/MMAC1 ( PTEN ) gene was identified as a tumor suppressor gene encoding a cytoplasmic protein that controls cellular processes. To investigate the potential role and the alteration of the PTEN gene in soft tissue sarcomas (STSs), we searched for homozygous deletion and promoter hypermethylation in a series of 48 STSs that was composed of malignant fibrous histiocytoma, leiomyosarcoma, malignant peripheral nerve sheath tumor, including 2 cases with a mutation that we previously reported; differential polymerase chain reaction and methylation-specific polymerase chain reaction, respectively, were used for the analyses. Furthermore, to determine whether PTEN gene alterations are involved in the down-regulation of PTEN expression, we examined the expression of PTEN protein in 38 cases in which paraffin-embedded tissues were available for immunohistochemical analysis. In addition to our previous results showing that 2 (4%) of 51 cases had a PTEN mutation, promoter methylation was recognized in 6 (13%) of 48 cases, and homozygous deletion was detected in 1 (2%) of 48 cases in the current study. Of 6 cases with promoter methylation of PTEN gene, 5 were malignant peripheral nerve sheath tumor. Decreased expression of PTEN protein was recognized in 11 (29%) of 38 STS cases. Of 9 cases with PTEN alterations (6 cases with promoter methylation, 2 with mutation, and 1 with homozygous deletion), 3 (33%) showed decreased expression of PTEN protein. Furthermore, decreased expression of the PTEN gene showed a statistically significant correlation with high MIB-1 labeling index in 38 STS cases examined ( P = .0441). In conclusion, promoter methylation and homozygous deletion of the PTEN gene were found to be relatively rare events in cases of STS, as is mutation of the gene. Of 9 cases with a PTEN alteration, 3 (33%) showed a decrease in PTEN expression, indicating that PTEN gene alterations seem to play a minor role in the inactivation of PTEN in these tumors. Furthermore, although a further detailed analysis of a larger number of cases is still necessary, the present results suggest that PTEN expression may be a useful indicator of cell proliferation in patients with STS. 相似文献
56.
前臂后皮神经营养血管远端蒂筋膜皮瓣修复手部软组织缺损 总被引:2,自引:0,他引:2
目的:探讨前臂后皮神经营养血管远端蒂筋膜皮瓣修复手部软组织缺损的临床效果。方法:对18例手部创伤性软组织缺损创面采用带前臂后皮神经营养血管远端蒂筋膜皮瓣进行修复,其中机器绞伤11例,压砸伤5例,电锯伤2例,皮瓣面积最大为12cm×8cm,最小7cm×5cm。结果:17例皮瓣完全成活;1例皮瓣边缘部分坏死,经清创换药痊愈。术后随访6~12个月,皮瓣质地良好,外观满意。结论:前臂后皮神经营养血管远端蒂筋膜皮瓣切取简便、成功率高,是修复手部软组织缺损的理想供区。 相似文献
57.
Monosomy 9q and trisomy 16q in a case of congenital solitary infantile myofibromatosis 总被引:1,自引:0,他引:1
Sirvent N Perrin C Lacour JP Maire G Attias R Pedeutour F 《Virchows Archiv : an international journal of pathology》2004,445(5):537-540
Although infantile myofibromatosis (IM) is the most common fibrous proliferation of infancy, many aspects of this benign lesion have not been explored. IM histogenesis is still poorly understood, despite immunohistochemical staining and ultrastructural features that suggest a myofibroblastic origin. IM diagnosis is often made difficult by the predominance of small primitive spindle cells over myofibrobasts and the presence of intravascular growth. Genetic information is scarce, with only one karyotyped case. Here we describe a case of solitary IM discovered at birth in an otherwise healthy girl. The tumor was well circumscribed, arranged in nodules and made up of ovoid cells without atypia, in a myxoid background. Immunohistochemical evaluation indicated a myofibroblastic differentiation. The cytogenetic and fluorescence in situ hybridization analyses revealed an abnormal chromosome 9, derived from an unbalanced whole-arm translocation between chromosomes 9 and 16. On both chromosomes, the breakpoints were located in the pericentric heterochromatic region. This clonal abnormality has not been reported in other tumors and is different from the chromosome 6q deletion reported in the single previous reported IM karyotype. 相似文献
58.
Seven cases of spindle-cell proliferations in which fine-needle aspiration biopsy (FNAB) did not correlate with subsequent histology are presented. Three cases were considered low-grade sarcoma, one a dermatofibrosarcoma protuberans (DFSP), one a spindle-cell tumor with malignancy not excluded, and one a rhab-domyosarcoma vs. a fibrosarcoma. Two of the these three were histologically nodular fasciitis and one an inflammatory pseudotumor. Two cases were diagnosed cytologically as fibromatosis or nodular fasciitis (NF). One of these histologically was an intramuscular hemangioma, the other a DESP. The last two cases were diagnosed by FNAB as spindle-cell lesion, undetermined if benign or malignant, and malignant fibrous histiocytoma (MFH). Histologically both of these case were leiomyosarcoma. The cyto-logic features of each case, differential diagnosis, and potential pitfalls are discussed. In the evaluation of FNAB smears dominated by spindle cells, cellullarity, individual cells and cell patterns,and background stromal features coupled with a precise clinical history may allow a narrow differential diagnosis with a focus on whether the lesion is benign or malignant. Caution is warranted in the exact classification of spindle-cell tumors from FNAB as this may have a major impact on patient management. © 1994 Wiley-Liss, Inc. 相似文献
59.
Extraskeletal myxoid chondrosarcoma: multimodal diagnosis and identification of a new cytogenetic subgroup characterized by t(9;17)(q22;q11) 总被引:2,自引:0,他引:2
Bjerkehagen B Dietrich C Reed W Micci F Saeter G Berner A Nesland JM Heim S 《Virchows Archiv : an international journal of pathology》1999,435(5):524-530
Extraskeletal myxoid chondrosarcoma is a rare malignant soft tissue tumour that can be difficult to diagnose correctly, especially
preoperatively. We describe four cases of extraskeletal myxoid chondrosarcoma of the extremities diagnosed by a multimodal
approach. The cytological examination of fine-needle aspirates showed small and round, mildly pleomorphic cells lying in sheets
and cords, but also dispersed within a myxoid and metachromatic intercellular substance. Histological, electron microscopic
and immunocytochemical examination also yielded findings compatible with the diagnosis of extraskeletal myxoid chondrosarcoma.
Cytogenetic analysis demonstrated a t(9;22)(q22;q12) in two tumours and a t(9;17)(q22;q11) in the third and fourth. The translocation
t(9;22)(q22;q12) has been described repeatedly in extraskeletal myxoid chondrosarcoma but never in other tumours; hence, the
detection of this pathognomonic chromosome abnormality in short-term cultured cells from fine-needle aspirates verified the
diagnosis in two of the cases. The t(9;17)(q22;q11) found in the last two cases probably represents a new cytogenetic subgroup
of extraskeletal myxoid chondrosarcoma as it, too, is unknown in other contexts. The multimodal approach taken in these four
cases enabled a definite diagnosis of a rare malignant tumour whose cytological and histological features alone are usually
not sufficiently distinct to rule out other differential diagnostic possibilities.
Received: 16 March 1999 / Accepted: 1 June 1999 相似文献
60.
软组织平滑肌肉瘤中p16基因的甲基化检测 总被引:2,自引:2,他引:2
目的 探讨软组织平滑肌肉瘤(LMS)中p16基因INK4A的甲基化状态及其与p16表达的关系。方法 应用MSP法检测38例软组织平滑肌肉瘤,10例平滑肌瘤及5例正常平滑肌组织中p16基因INK4A的甲基化状态,用免疫组织化学SP方法检测p16蛋白表达情况。结果 38例LMS中9例发生异常甲基化,异常甲基化率为23.7%(9/38)。其中,7例p16蛋白表达阴性,2例p16蛋白弱阳性,在p16蛋白表达阴性的LMS中,异常甲基化率为50%(7/14)。结论 p16基因第一外显子启动子区5‘CpG岛的异常甲基化是导致p16基因失活、蛋白缺如的重要基因外机制,并可能参与肿瘤的发生。 相似文献