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91.
Carol A. Sawka Kathleen I. Pritchard Wendy Shelley Gerrit DeBoer Alexander H.G. Paterson J. William Meakin Donald J.A. Sutherland 《Breast cancer research and treatment》1997,43(3):211-215
Background: The outcome of breast cancer is usuallydetermined by multiple factors. Serum tumor necrosis factoralpha concentration has been found to be increasedin the circulation of patients with malignancy. Thisstudy was designed with the aim to investigateany correlation between the serum tumor necrosis factoralpha and the clinicopathological fetures and furthermore evaluatethe prognostic significance of serum tumor necrosis factoralpha concentration in breast cancer. Methods: Forty consecutivepatients with invasive breast cancer undergoing modified radicalmastectomy were prospectively included and evaluated. Venous bloodsamples were collected before the surgery. Sera wereobtained by centrifugation, and stored at – 70°C until assayed. The control group consisted 30healthy, age-matched subjects. Serum concentrations of tumor necrosisfactor alpha were measured by the quantitative sandwichenzyme immunoassay technique. The data on tumor size,age, estrogen receptor status, lymph node status andTNM staging were reviewed and recorded.Results: The mean value of serum tumor necrosis factor alphain patients with invasive breast cancer was 1.47± 0.58 pg/ml and that of the controlgroup was 0.98 ± 0.37 pg/ml, and thedifference was significant (P < 0.01). With univariableanalysis, patients with maximum tumor size of 5cm or larger (P=0.03), more advancedTNM staging (P < 0.01); and more advancedlymph node status (P < 0.01) were shownto have significantly higher serum concentrations of tumornecrosis factor alpha. However, with multivariable analysis, TNMstaging appeared as the only independent factor (P< 0.01) predicting the significant, higher serum concentrationsof tumor necrosis factor alpha. Conclusion: Preoperative evaluationof serum tumor necrosis factor alpha concentrations maybe a valuable parameter for reflecting the severityof staging for invasive breast cancer. 相似文献
92.
Binding of sulfaethidole to bovine serum albumin was studied by equilibrium dialysis, fluorescence probe technique, uv difference
spectrophotometry and circular dichroism. Equilibrium dialysis method enabled us to estimate the total number of drug binding
sites of albumin molecule. For sulfaethidole, albumin had 6 primary and 40 secondary binding sites. The primary and secondary
binding constants were 0.9×105 M−1 and 0.2×106 M−1, respectively. 1-Anilino-8-naphthalenesulfonate (ANS) and 2-(4′-hydroxylbenzeneazo)-benzoic acid (HBAB) were used as the
fluorescence probe and the uv spectrophotometric probe, respectively. In fluorescence probe technique, results indicated that
the number of higher affinity drug binding site of albumin was 1 and the number of lower affinity drug binding sites of albumin
was 3, and the primary and secondary drug binding constants for bovine serum albumin were 2.15×105 M−1 and 1.04×105 M−1, respectively. In uv difference spectrophotometry, binding sites were 3 and binding constant was 1.88×105 M−1. The above results suggest that several different methods should be used in ompensation for insufficient information about
drug binding to albumin molecule given by only one method. 相似文献
93.
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95.
应用人自体血清培养人口腔黏膜上皮的实验研究 总被引:1,自引:0,他引:1
目的 研究人自体血清培养人口腔黏膜移植生长的生物学特性,为组织工程化尿道提供新材料。方法 将人自体血清培养黏膜移植于裸鼠体内,分别于移植后2、3、4、6周观察培养黏膜生长与转归,应用anti—HLA免疫荧光鉴定成活黏膜组织属性,应用抗人Ⅳ型胶原及抗人层黏蛋白为基底膜形成指标。结果 裸鼠体内移植培养黏膜成活生长分化良好,anti—HLA免疫荧光证实为移植的培养人黏膜组织;免疫组化发现移植后3周开始形成基底膜,4周形成完整的基底膜。结论 自体血清培养的人口腔黏膜可形成功能完整的上皮组织。 相似文献
96.
Yuichi Yamada Teruhiko Kido Masao Ishizaki Ryumon Honda Ikiko Tsuritani Haruki Yamaya Kôji Nogawa 《International archives of occupational and environmental health》1988,60(1):7-14
Summary Forty-nine out of 54 male workers engaged in the production of an epoxy compound, t-methyl-3-phenylglycidate, showed skin symptoms in varing degrees that may be due to the skin-irritative effect of the compound. The exposed workers were also shown to have subjective symptoms which may be related to the irritative property of the compound on surface tissue. Laboratory examinations on the blood obtained from the exposed workers showed significantly higher values of leukocyte concentration as compared with the non-exposed controls. This was chiefly caused by the increase of neutrophilic granulocytes and T-cell lymphocytes. Serum IgA levels of the exposed workers were shown to be significantly lower than those of the control group. Hemoglobin concentration, hematocrit value and red cell count of the exposed workers remained at the same level as those of the control subjects. Liver or kidney damage was not found in biochemical analyses on the sera of exposed workers. 相似文献
97.
目的:探讨中国妇女哺乳期使用迪波盖司通后血清和乳液醋酸甲羟孕酮(MPA)浓度变化。方法:10名产后哺乳妇女单次注射迪波盖司通(含MPA150mg),在注射后的第1、2、4、6、8、10和12周采集血样和乳液样本,用放射免疫方法测定MPA。结果:血清MPA浓度于注射后第1周最高,到第2、4周时下降明显,第4周后浓度下降趋势逐渐缓慢。乳液MPA浓度在第1周为最高,第2周比第1周降低了约1/2,之后10周平均浓度变化波动在5.09-8.15ng/ml之间。观察期间乳液/血清MPA浓度比值和曲线下浓度面积比值均为0.55。对象之间和同一对象不同时间点乳液/血清MPA浓度存在明显个体差异。结论:哺乳期使用迪波盖司通,将导致血液和乳液中含有一定量的MPA。 相似文献
98.
99.
目的 探讨血清瘦素与血压、体重指数 (BMI)、腰围、臀围、腰臀比 (WHR)及血脂、血尿酸等因素的关系。方法 测定 6 8例高血压患者及 4 8名正常人的血清瘦素浓度、血压、人体测量变量值及生化指标。血清瘦素用ELISA法测定。结果 (1)两组血清瘦素浓度无显著性差别 (P >0 .0 5 ) ,肥胖者血清瘦素浓度显著于非肥胖者 (P<0 .0 0 1) ,女性显著高于男性 (P <0 .0 0 1) ;(2 )高血压组血清瘦素与BMI、血尿酸 (URIC)、总胆固醇 (TC)、腰围、臀围、WHR显著正相关 ;经多元回归分析 ,血清瘦素浓度的影响因素为性别、BMI、URIC、WHR。结论 血清瘦素浓度与血压、年龄无关 ,而与BMI、腰围、臀围、WHT、URIC显著相关。 相似文献
100.
在整体观和平衡观的基础上 ,创立双梯度聚丙烯酰胺凝胶电泳法 ,应用于同步分离血清脂蛋白及其亚组分 ,检测血清脂蛋白谱 (SLPG) ,以及“内脾虚”的基础和临床研究 ,并逐步建立了新的理论体系。SLPG较理想地表达了血清脂蛋白动态平衡的状态 ,是中西医结合认识血脂代谢和“脾气散精”功能的新指标。实践提示 ,有机结合和共同发展应是现代中西医结合研究面临的主要任务之一。 相似文献