穿心莲旱地绿色栽培技术的研究

目的 规范穿心莲旱地绿色栽培技术,提高旱地栽培穿心莲药材的产量和质量.方法 在公司穿心莲旱地种植示范基地,近年来开展了一系列穿心莲旱地绿色栽培技术的研究.结果 在田间试验及示范基地中试与推广的基础上,总结出穿心莲旱地绿色栽培技术.结论 这一技术对于在粤北山区丘陵坡地建设穿心莲规范化生产基地,提高穿心莲药材产量和质量具有...     

穿心莲的药理作用研究进展

综述了近年来穿心莲的药理作用研究情况,表明穿心莲具有抗炎、抗癌、抗心血管疾病、抗病毒、抗菌、提高机体免疫力、保肝利胆、抗生育方面药理作用,值得进一步研究开发。     

一测多评法测定穿心莲及其制剂中内酯类成分

目的 建立穿心莲及其制剂消炎利胆片的一测多评定量测定方法。方法 在建立穿心莲4种主要内酯类成分同步测定方法的基础上,测定新穿心莲内酯、去氧穿心莲内酯和脱水穿心莲内酯与穿心莲内酯的相对校正因子,考察其重现性,并在穿心莲药材及消炎利胆片中加以应用;同时采用外标法测定其中4种内酯类成分的量,并比较计算值与实测值的差异。结果 用一测多评法对39批穿心莲药材和30批消炎利胆片中4种内酯类成分进行测定,药材测定计算值与实测值（外标法）相对误差＜5%,消炎利胆片测定计算值与实测值（外标法）相对误差＜8%。结论 同步测定穿心莲内酯、新穿心莲内酯、去氧穿心莲内酯和脱水穿心莲内酯的一测多评法可用于穿心莲药材及消炎利胆片的质量控制。     

Protective activity of andrographolide and arabinogalactan proteins from Andrographis paniculata Nees. against ethanol-induced toxicity in mice

To find out the active principles against ethanol-induced toxicity in mice, Andrographis paniculata Nees. (Ap) was chosen and isolated andrographolide (ANDRO) and arabinogalactan proteins (AGPs). ANDRO was detected by HPTLC, FTIR and quantified by HPLC (10mg/g of Ap powder). AGPs was detected by beta-glucosyl Yariv staining of SDS-PAGE gel, FTIR and quantified by single radial gel diffusion assay with beta-glucosyl Yariv reagent (0.5mg/g Ap powder). The mice are pretreated intra-peritoneally (i.p.) with different doses (62.5, 125, 250, and 500mg/kg) of body weight of mice] of ANDRO and AGPs for 7 days and then ethanol (7.5g/kg of body weight) was injected, i.p. Besides, silymarin was used as standard hepatoprotective agent for comparative study with ANDRO and AGPs. The ameliorative activity of ANDRO and AGP against hepatic renal alcohol toxicity was measured by assessing GOT, GPT, ACP, ALP and LP levels in liver and kidney. It has been observed that pretreatment of mice with ANDRO and AGPs at 500mg/kg of body weight and 125mg/kg of body weight respectively could able to minimize the toxicity in compare to ethanol treated group as revealed by the different enzymatic assay in liver and kidney tissues and the results were comparable with silymarin. Hence, out of several ill-defined compounds present in Ap, ANDRO and AGPs are the potential bioactive compounds responsible for protection against ethanol-induced toxicity.     

Andrographolide inhibits the tumour necrosis factor-α-induced upregulation of ICAM-1 expression and endothelial-monocyte adhesion

Andrographolide, a diterpene isolated from Andrographis paniculata, has been shown to have several biological activities including analgesic, antipyretic and antiinflammatory effects. Since the upregulation of adhesion molecules expression and endothelial-leucocytes adhesion are key steps in the development of inflammation, the present study examines whether andrographolide modulates these biological processes in vitro. Incubation of endothelial cells with non-toxic concentrations (0.16–16.7 υg/mL) of andrographolide attenuated the tumour necrosis factor-α (TNF)-induced intercellular adhesion molecule-1 (ICAM-1) expression. Similar concentration ranges of andrographolide also inhibited the TNF-induced endothelial-monocyte adhesion in a concentration-dependent manner. These effects of andrographolide may account for its reported in vivoantiinflammatory activity. © 1998 John Wiley & Sons, Ltd.     

肺靶向穿心莲内酯脂质体的制备及体外释放特征

目的：研究肺靶向穿心莲内酯脂质体的制备方法并考察其体外释药性质。方法：采用薄膜一冻融法制备穿心莲内酯脂质体;高效液相色谱法测定包封率;用透析法考察药物体外释放性质。结果：制得的脂质体平均粒径为6．325μm,包封率大于75％,稳定性好。药物体外释放符合Higuchi方程。结论：采用薄膜．冻融法可制得具有较高包封率及稳定性的穿心莲内酯脂质体,有助于提高穿心莲内酯的肺靶向性。     

印尼复方穿心莲发酵蜂蜜汤剂“JAMSI”对高血糖患者的即刻降糖功效临床验证

目的:研究印尼复方穿心莲发酵蜂蜜汤剂"JAMSI"(由印尼PT Mahkotadewa Indonesia公司生产;印尼食品药品管理局注册号:TR053649111)对高血糖患者的即刻降糖功效。方法:本研究设计为对高血糖患者治疗前对治疗后的降糖功效评价,研究患者来自参观本产品展览台的来宾,他们必须患有高血糖或糖尿病并愿意参与本研究。检测临时毛细血管血血糖使用德国制造的Accu-Chek Active血糖检测仪在服用JAMSI汤剂前及服用后1 h。所获得的配对血糖数据按统计学一边t检验法分析并总结,统计学意义界限α=0.05。结果:在两次展览中即2013年2月16—23日在雅加达,获得34位合格患者为本研究患者,性别上分为20女和14男,其中20位正在服用降血糖西药。患者年龄最小22岁,最老74岁,平均(52.26±10.10)岁,糖尿史由1~33年,平均(9.36±8.27)年。患者的平均毛血管血血糖浓度在服用JAMSI汤剂前是(243.03±97.97)mg/dL,而服用后1 h是(197.94±100.01)mg/dL,两者差异有统计学意义(P0.01)。按服用前血糖浓度高于200 mg/dL的患者对比服用前血糖浓度低于200 mg/dL的患者分析结果显示服用JAMSI后降低血糖幅度在前者比后者更明显(表3)。而在正在服用降血糖西药者与没有服用降血糖药者之间降血糖幅度差异无统计学意义(P0.05)。结论:受检验的印尼复方穿心莲发酵蜂蜜汤剂"JAMSI"显示非常显著的即刻降血糖功效;此外在被研究的患者中没有发现任何严重不良反应。有关该药剂的中长期效果有待继续研究证明。     

Analysis on Isozyme and Diterpene Lactones Variability during Ontogenesis of Andrographis paniculata

Objective To understand the relationship between isozyme activities and diterpene lactone biosynthesis of Andrographis paniculata. Methods Plants were collected during ontogeny from seeding to seed maturity, and separated into leaves and stems for determination. Morphological and yield parameters were used to describe plant growing states. Isozyme changes were tested by polyacrylamide gel electrophoresis. HPLC was used to develop the fingerprints as well as to determine the diterpene lactone content. Results Significant increases were observed in the activities of isozymes, such as aspartate aminotransferase(AST), malate dehydrogenase(MDH), peroxidase(POD), and catalase(CAT), around the early stage of bud in leaves, and the activities of these four kinds of isozymes increased gradually as time progressed in stems. The content changes of diterpene lactones in leaves and stem were various. In the leaves, andrographolide(1) was recorded the highest [(23.63 ± 1.06) mg/g] at the early stage of bud, whereas deoxyandrographolide(2) was the lowest [(6.78 ± 0.27) mg/g] at this period and it reached the highest level at the seeding stage [(26.05 ± 1.04) mg/g]. Dehydroandrographolide(3) and neoandrographolide(4) fluctuated during growing stages. Meanwhile, the HPLC fingerprint showed that the content changes of two unknown compounds were related to that of dehydroandrographolide in leaves. In stems, andrographolide had increased gradually until the bud stage [(8.26 ± 0.33)mg/g], and other three diterpene lactones showed a trend of fluctuation. The yield of total diterpene lactones in aerial part reached the highest at the first flowering stage(806.71 mg/plant). Conclusion These results lay the foundation for the future research on the relationship of isozymes and diterpene lactones, and for determining the most favorable time for harvesting A. paniculata.     

穿心莲指纹图谱的实验研究

目的:建立代表穿心莲的指纹特征指纹图谱检测方法,控制穿心莲药物的质量.方法:在特定的色谱条件下,采用高效液相色谱法测定穿心莲药材的指纹图谱,并以中药色谱指纹图谱的相似度评价系统操作规范软件进行评价.结果:通过高效液相色谱法建立了稳定性好、精密度高、重复性良好的指纹图谱检测方法.结论:建立的穿心莲指纹图谱检测方法可用于的其药材鉴别及质量控制.