[Ca^2＋]i change in hippocampal neurons influenced by preconditioning of etomidate fat emulsion following cerebral ischemia in rats

BACKGROUND: It is known that intravenous anesthetic etomidate fat emulsion has cerebral protection. Now many scholars focus on the research of its cerebral protection from molecular biology, but the mechanism of cerebral protection is still fully unclear. OBJECTIVE: To observe the influence of etomidate fat emulsion on the [Ca2+]i in hippocampal neurons during the transient cerebral ischemia injury in rats. DESIGN: Randomized controlled observation. SETTING: Weifang Medical College. MATERIALS: This study was carried out in the functional laboratory of Weifang Medical College between October 2005 and March 2006. Twenty-four male healthy Wistar rats, aged 3 to 4 months, were involved. Etomidate fat emulsion was provided by the limited company of En-hua Medical Bloc in Jiangsu Province (code of H20020511) and the other agents and materials were provided by Laboratory Center of Weifang Medical College. METHODS: The 24 Wistar rats were randomized into 3 groups: sham-operation group, model group and etomidate preconditioning group, with 8 rats in each. Rat models of transient cerebral ischemia injury were made by the ligation of bilateral carotid arteries combined with descending blood pressure in the latter two groups. Before ischemia (ligation of bilateral common carotid artery), rats in the etomidate preconditioning group were intraperitoneally injected with 12 mg/kg etomidate fat emulsion and then persistently intraperitoneally injected with etomidate fat emulsion at 1.0 mg/kg per minute. Rats in the model group were not administrated. Rats in the sham-operation group were only performed bilateral common carotid artery isolation. When rats were modeled, their brain tissues were quickly taken out and detected. MAIN OUTCOME MEASURES: Change of the fluorescence pixel value of the [Ca2+]i in each group by the laser scanning confocal microscope. RESULTS: Twenty-four rats were involved in the final analysis. Fluorescence pixel value in the sham-operation group was in the low level. Fluorescence pixel value in the model group was significantly higher than that in the sham-operation group (P < 0.01). Fluorescence pixel value in the etomidate preconditioning group was significantly lower than that in the model group (P < 0.01). CONCLUSION: The protection of etomidate fat emulsion to the transient cerebral ischemic injury in rats is associated with the inhibition to the increase of [Ca2+]i to some extent.     

血管通透性的调节和游离微血管技术在其研究中的应用

Vascular hyperpermeability is a cardinal feature of inflammation or bum in which an array of inflammatory mediators can cause such changes in the microvessels. The fimctional measures of microvascular exchange that represent the properties of microvascular wails are the permeability coefficients which have been reported from measurements on intact whole organisms (including human subjects}, on perfused tissues and organs, on single perfused microvessels, and on monolayers of cultured microvascular endothelial cells. In this review, we summarize some experiments of vascular permeability in individually isolated perfused microvessels.     

As_2O_3诱导人肝癌细胞凋亡及对Fas表达和钙含量的影响

化疗在原发性肝癌的综合治疗中占重要地位.为寻找肝癌治疗新的有效药物,我们观察了As2O3对人肝癌细胞株BEL7402的生物学效应,以期为As2O3临床治疗肝癌提供实验依据.     

光化学诱导树鼠句局部血栓形成后中心区和半暗区 rCBF 及神经元泵功能的变化(摘要)

光化学诱导树鼠句局部血栓形成后中心区和半暗区ｒＣＢＦ及神经元泵功能的变化（摘要）研究生张利能导师李树清李麟仙（昆明医学院病理生理教研室，昆明６５００３１）关键词光化学，脑血栓形成，半暗区，局部脑血流，脑水肿，钙离子中图分类号Ｒ３６５缺血性脑卒中是临...     

Protective Effects of Calcium Antagonists on Cadmium—induced Toxicity in Rats

INTRODUCTIONCadmium,oneoftheimPOrtantenvironmentalpollutants,isknowntocausere-nal,hepatic,neurologicandtesticulardisorders.ThemechanismofheavymetaItoxi-cityremainsunclearalthoughmuchresearchhasbeendonesincethe192Os.Anditwasreportedthatthemetalchelatorsusedinthetherapyofsuchheavymeta1induceddiseasesmaycausesideeffectsonthepatients(Knandelwaletal.,l987).Recently,anewhypothesissuggestedthatcalmodulin(CaM),theuniquemodulatorproteinofcalciumdependentfunctions,mightbethefirsttargetmoleculefo…     

汽车排气对交通警及隧道工健康的影响

以上海市四个区的交通警184人,打浦路隧道工58人为接触组,植物园工人106人为对照组,研究了汽车排气对其健康的影响。发现隧道工、交通警的主诉症状、客观体征和铅接触的生化指标方面与对照组比较,有显著差别(P<0.05及0.01)。不论吸烟与否,隧道工血中HbCO含量的最高值都是在值勤后,系因值勤时吸入过量的CO所致。说明汽车排气对交通警及隧道工人的健康已产生一定的影响。     

异搏定对胰腺腺泡细胞内游离钙离子浓度的影响

目的:探讨细胞内钙超负荷在急性胰腺炎(AP)发生发展中的作用.方法:使用140只SD大白鼠胆胰管逆行加压注射4.5%牛磺胆酸钠制成AP模型,用荧光指示剂Fura-2/Am测定游离胰腺腺泡细胞内游离钙离子浓度([Ca~(2 )]i).结果:注射后2小时和3小时胰腺呈急性出血坏死性炎症早期改变,制AP模型后AP组[Ca~(2 )]i较对照组明显增高(P<0.001),AP组胰腺腺泡细胞内[Ca~(2 )]i增高值与胰腺病理变化程度呈正相关关系(r_s=0.9727,P<0.001),异搏定治疗可明显提高病鼠生存率和改善胰腺组织出血坏死程度和腺泡细胞超微结构损害.结论:胰腺腺泡细胞内钙超负荷在胆汁性急性水肿性胰腺炎向出血坏死性胰腺炎转变中起明显作用,钙通道阻滞剂异搏定可明显阻止胰腺细胞内钙离子超负荷,是其治疗急性胰腺炎的主要机理之一.     

膝关节鼠2例报告

自1980年以来，我院骨科收治两例“膝关节鼠”病人。术后7天痊愈出院，现报告如下。临床资料此2例病人，皆是男性，龄19岁和20岁。发病前，皆有膝关节撞伤史。平时膝关节活动自如，某些体位时，突然出现膝关节疼痛、交锁症状，慢慢活动后可缓解。有时膝关节表皮位可出现隆起之肿块。X线片示膝关节骨质无明显改变，诊断为“膝关节鼠”。治疗方法采用小切口方法，能快速取出游离体。术前让患者伸屈患侧膝关节，使游离骨块移到距皮肤最近的部位，用指可触们到后，将膝关节固定在屈曲位。消毒，铺巾，局麻，在肿块处皮肤作一1～Zcm／J’切口，…     

尼莫地平在治疗神经系统疾病中的应用

尼莫地平是一种选择性钙通道阻滞剂,能抑制病理性过量钙离子向细胞内流入,阻止因细胞内钙离子超载所引起的细胞水肿、坏死和死亡,抑制血管的持续收缩,降低血粘度而保护脑组织。本品对脑血管痉挛、脑梗塞、偏头痛、外伤性脑水肿有较好疗效。亦有人将本品用于治疗癫痫、病毒性脑炎、脑复苏的病人。本品毒副反应小。