原花青素交联牛心包材料的研究

本研究针对天然交联剂原花青素处理牛心包材料的性能进行研究.采用原花青素交联牛心包组织,制备人工生物心脏瓣膜材料,并对其交联特性、力学特性、抗酶降解性能、亲疏水性能、细胞毒性试验以及溶血试验等进行分析.结果显示:原花青素或戊二醛处理的牛心包组织变性温度、力学特性、表面亲水性能和抗酶降解能力都明显提高;与传统交联剂戊二醛相比组织稳定性、亲水性能和抗酶降解能力未见显著性差异,但是最大断裂强度显著提高(原花青素交联组织最大断裂强度(13.863 0 MPa)明显高于戊二醛交联组织(10.784 2 MPa);溶血试验结果表明原花青素处理固定组织的溶血率(2.61%)远低于戊二醛处理的组织(12.54%);细胞毒性试验结果表明原花青素交联组织在1、3、5 d的细胞增殖率分别为76.19%、88.96%、100.12%,而戊二醛在相同的时间点的细胞增殖率分别为63.15%、57.28%、48.74%.原花青素交联的瓣膜材料组织结构稳定、亲水性好、毒性小且能维持较好的力学性能,作为人工生物瓣膜材料具有很好的研究前景.     

原花青素对氨基脲染毒小鼠肾脏损伤的影响

目的:探讨原花青素(PC)对氨基脲(SEM)染毒小鼠肾脏损伤的影响。方法:50只成年昆明种(KM)小鼠,随机分为5组:溶剂对照组,SEM染毒组,SEM染毒+低、中、高剂量PC保护组;溶剂对照组,灌胃去离子水,每天1次,连续6周;SEM染毒组,前两周灌胃56.25 mg/(kg.bw)SEM溶液,后4周灌胃去离子水;低、中、高剂量PC保护组,前2周均灌胃56.25 mg/(kg.bw)SEM溶液,后4周分别灌胃100 mg、200 mg、400 mg/(kg.bw)PC溶液。末次灌胃24 h后,摘眼球采血后处死小鼠,测定相关指标。结果:SEM染毒组小鼠肾脏脏器系数低于溶剂对照组(P<0.05);中、高剂量PC保护组小鼠肾脏脏器系数均高于SEM染毒组(P<0.05);SEM染毒组肾组织形态,可见系膜细胞中、重度增生,肾小管内出现蛋白管型及大量巨噬细胞浸润;中、高剂量PC保护组,肾小管上皮细胞变性及炎性浸润等病理变化明显改善;SEM染毒组小鼠血清UREA、CRE、UA含量高于溶剂对照组(P<0.05),中、高剂量PC保护组UREA、CRE、UA含量均低于SEM染毒组(P<0.05);与溶剂对照组比较,SEM染毒组小鼠肾脏中的SOD活性下降、MDA含量增高(P<0.05),中、高剂量PC保护组SOD活性升高,MD含量降低与SEM染毒组相比,差别均有统计学意义(P<0.05)。结论:原花青素对氨基脲引起的小鼠肾损伤有保护作用,原花青素对氨基脲致小鼠肾损伤的保护机制与抗氧化损伤有关。     

正交试验优化金荞麦抗癌成分提取工艺的研究

目的 优选金荞麦抗癌有效成分最佳提取工艺.方法 采用正交实验,考察了溶剂种类、溶液pH值、提取温度、提取时间对金荞麦中抗癌成分提取量的影响.采用紫外分光光度法测定提取物中总原花青素含量,HPLC法测定提取物中原花青素B2的含量.结果 对总原花青素及原花青素B2提取溶剂种类、溶液pH值、提取温度是主要影响因素,均具有统计...     

葡萄籽原花青素对血管性痴呆大鼠空间学习记忆能力的影响

目的观察葡萄籽原花青素(GSP)对血管性痴呆(VD)大鼠空间学习记忆能力的影响,探讨其可能的作用机制。方法采用改良Pulsinelli四血管阻塞法建立VD大鼠模型,造模成功后,每天灌胃给药,Wistar大鼠分为假手术组,模型组,原花青素组(50 mg/kg和150 mg/kg),银杏叶片24 mg/kg。Morris水迷宫检测VD大鼠空间学习记忆能力;比色法检测脑组织超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)、谷胱甘肽(GSH)、丙二醛(MDA)含量;实时荧光定量PCR(Real-time PCR)法检测海马Bax、Bcl-2 mRNA的表达。结果与模型组比较,原花青素可明显改善VD大鼠空间学习记忆能力;提高脑组织SOD活性、GSH含量和总抗氧化能力,降低MDA含量;提高海马Bcl-2/Bax mRNA比值。结论原花青素可改善VD大鼠学习记忆功能,其机制可能与提高机体抗氧化能力、抑制海马神经元凋亡有关。     

原花青素对D-半乳糖复合三氯化铝诱导大鼠主动脉损伤的保护作用及机制

目的：探讨原花青素对D-半乳糖复合三氯化铝诱导大鼠主动脉损伤的保护作用及可能机制。方法： 采用D-半乳糖（D-gal）腹腔注射（ip）180mg/kg/d 联合三氯化铝（AlCl3）灌胃（ig）15mg/kg/d建立主动脉损伤大鼠模型,12周后将40只大鼠随机分为空白对照组（NS ip,NS ig）,主动脉损伤组（D-gal ip ＋ AlCl3 ig）,原花青素低、高剂量组（PC 20、40 mg/kg; D-gal ip＋AlCl3 ig）,维生素E组（VitE 10 mg/kg;D-gal ip ＋ AlCl3 ig）,每组8只。每日灌胃给药一次,连续8周。于末次给药后,尾袖法测量大鼠尾动脉血压;离体灌流法观察主动脉环对乙酰胆碱的舒张反应;HE染色观察主动脉病理变化;化学比色法测定血清总抗氧化能力、过氧化氢以及一氧化氮含量;免疫组化法观察主动脉内皮型一氧化氮合酶（eNOS）表达。结果：与模型组相比,随着原花青素剂量的增加,大鼠血压逐渐降低,主动脉内皮依赖性舒张功能逐渐增强,主动脉病理损伤得到不同程度的改善;血清中T-AOC 和NO含量逐渐增高,H2O2含量逐渐降低;主动脉eNOS蛋白表达逐渐增加。结论：原花青素可明显降低主动脉血压,增强主动脉环内皮依赖性舒张反应,减轻主动脉病理损伤,增高血清NO水平,上调主动脉eNOS蛋白的表达,对D-半乳糖复合三氯化铝诱导的大鼠主动脉损伤有一定保护作用。     

成年女性血锌血硒水平调查分析

目的:探讨成年女性血锌血硒水平。方法:应用原子吸收分光光度计检测锌、硒。结果:成年女性锌缺乏率为23.8%,其中20岁至69岁女性锌缺乏率为20.8%,70岁至90岁女性为42.4%,二者差异显著。结论:成年女性应定期检测,维持血锌血硒在正常水平。     

The chemoadjuvant potential of grape seed procyanidins on p53-related cell death in oral cancer cells

J Oral Pathol Med (2012) 41 : 322–331 Background: To clarify the efficacy of grape seed procyanidin (GSP) on antiproliferative effects related to p53 functional status of oral squamous cell carcinoma (OSCC) for its chemoadjuvant potential. Methods: We used GSP to investigate SCC‐25 cells with wild‐type p53 gene and OEC‐M1 cells with mutant p53 gene for the assessment of antiproliferative effects including cell viability, cell cycle, apoptosis, migration and invasion potential, and alterations of associated oncoproteins involved in cellular and molecular events. Results: The findings suggest that GSP on OEC‐M1 cells leads to cell cycle arrest by increasing the expression of p21^Cip1/p27^Kip1 protein without functioning mitochondria‐mediated apoptosis, whereas GSP on SCC‐25 cells inhibits cell proliferation via both G1‐phase arrest and mitochondria‐mediated apoptosis in a dose‐dependent manner as a result of alterations of Bcl‐2. GSP also inhibits the migration and invasion of both cells, which are associated with the suppression of matrix metalloproteinases (MMPs), MMP‐2 and MMP‐9. Conclusion: Antiproliferative effectiveness of GSP is closely associated with the p53 status of OSCC cells. GSP displays chemoadjuvant potential via cell cycle blockage and apoptotic induction. Our findings clearly suggest that GSP may play a role as a novel chemopreventive or therapeutic agent for OSCC.     

不同再矿化处理对漂白后釉质表面形貌及钙磷比的影响

目的 探究不同再矿化处理对漂白后釉质表面形貌及钙磷比的影响。方法 制备牛牙釉质样本,漂白处理后,随机分为5组:去离子水(DW)组;2%氟化钠(NaF)组;10%原花青素(PC)组;酪蛋白磷酸肽-无定形磷酸钙(CPP-ACP)组;PC+CPP-ACP组。用色差仪、扫描电镜、能谱仪和原子力显微镜分别检测样本漂白前、漂白后即刻及再矿化1周的颜色、表面形貌、元素和粗糙度变化。结果 各组间色差值无统计学意义(P>0.05)。扫描电镜显示,再矿化后各组均可见程度不一的矿物质沉积,PC+CPP-ACP组沉积物更明显。能谱仪显示,漂白后釉质表面钙磷比降低,再矿化后均有升高,NaF及PC+CPP-ACP组更高(P<0.05)。原子力显微镜显示,再矿化后各组均可见沉积物且粗糙度降低(P<0.05),PC+CPP-ACP组沉积物更明显、粗糙度更小(P<0.05)。结论 PC和CPP-ACP均能促进漂白后釉质再矿化,联合作用效果更佳。PC和CPP-ACP没有影响漂白效果。     

Extracts,Anthocyanins and Procyanidins from Aronia melanocarpa as Radical Scavengers and Enzyme Inhibitors

Extracts, subfractions, isolated anthocyanins and isolated procyanidins B2, B5 and C1 from the berries and bark of Aronia melanocarpa were investigated for their antioxidant and enzyme inhibitory activities. Four different bioassays were used, namely scavenging of the diphenylpicrylhydrazyl (DPPH) radical, inhibition of 15-lipoxygenase (15-LO), inhibition of xanthine oxidase (XO) and inhibition of α-glucosidase. Among the anthocyanins, cyanidin 3-arabinoside possessed the strongest and cyanidin 3-xyloside the weakest radical scavenging and enzyme inhibitory activity. These effects seem to be influenced by the sugar units linked to the anthocyanidin. Subfractions enriched in procyanidins were found to be potent α-glucosidase inhibitors; they possessed high radical scavenging properties, strong inhibitory activity towards 15-LO and moderate inhibitory activity towards XO. Trimeric procyanidin C1 showed higher activity in the biological assays compared to the dimeric procyanidins B2 and B5. This study suggests that different polyphenolic compounds of A. melanocarpa can have beneficial effects in reducing blood glucose levels due to inhibition of α-glucosidase and may have a potential to alleviate oxidative stress.