无名异冲剂促进去卵巢大鼠骨质疏松性骨折愈合的机制研究

为探讨无名异冲剂促进去卵巢 SD大鼠骨质疏松性骨折愈合的机制 ,用 48只 6月龄雄性 SD大鼠建立骨质疏松性骨折模型 ,随机分为模型组、跳骨片组、仙灵骨葆组、无名异冲剂组 ,于灌胃后 2周、4周取材 ,观察 X线片、骨密度、光镜 ,骨痂钙、磷、胶原含量 ,结果显示灌胃第 2周时 ,各组动物各项指标均无显著性差异 ;第4周时 ,无名异冲剂组的骨密度 ,骨痂钙、磷、胶原含量的检测结果与模型组有显著性差异 ( P<0 .0 5 ) ,X线及光镜观察骨痂的生长情况 ,无名异冲剂组也优于模型组 ,说明无名异冲剂能够通过提高骨密度 ,改善骨骼钙、磷、胶原的代谢 ,促进骨折端骨痂的生长 ,达到治疗骨质疏松性骨折的目的。     

Direct cadiac electorphysiologic effects of sufentanil and vecuronium in isolated guinea-pig hearts

Sufentanil and vecuronium are commonly used simultaneously in anaesthesia. Bradycardia and asystole have been described immediately after the administration of these two compounds. Therefore, the purpose of the present study was to evaluate the direct cardiac effects of sufentanil and vecuronium in all parts of the cardiac pacemaker and conduction system.
The electrophysiological effects of sufentanil and vecuronium were studied in isolated spontaneously beating guinea-pig hearts perfused by the method of Langendorff. At a concentration of 0.1 μmol/1 sufentanil a significant reduction of the spontaneous sinus rate, prolongation of atrioventricular, intraventricular and His' bundle conduction could be observed. The highest concentration of 10 μmol/1 of sufentanil led to an overall slowing of conduction velocity and to an profound slowing of spontaneous sinus rate. AV nodal as well as atrial and ventricular refractoriness were markedly prolonged at this high concentration of sufentanil. In contrast, during perfusion with vecuronium at a concentration of 0.1 μmol/1 up to 10 μmol/1 no significant effects on cardiac conduction and pacemaker activity could be observed.
In conclusion, the electrophysiological effects of sufentanil are comparable to that of unspecific calcium antagonists. Therefore, especially in patients with a preexisting damage of the cardiac conduction system, the indirect effect of the combination of sufentanil and vecuronium which is predominantly responsible for bradycardia and asystole may be worsened by the direct effects of sufentanil.     

Quality and standardization in blood component preparation with an automated blood processing technique

The use of automated blood processors in combination with bottom and top blood containers has been found to improve the standardization and quality of blood components. A study was performed to validate a new type of processor (Optipress® II) and compare its performance with a first generation processor (Optipress® I).
Primary separation on the Optipress II was investigated on 570 mL (± 10%) of anticoagulated blood in a nonpaired study. In addition, the quality of the products in routine production was compared between the results of the Optipress I and Optipress II. The whole blood units were kept overnight at room temperature (20 ± 2 °C). Separation was performed under conditions to obtain 55 mL buffy coats with a 50% haematocrit (ht). Platelet concentrate preparation was investigated in a paired study and compared to the routine manual method using PAS II additive solution. Parameters studied were volume, red cell, white cell and platelet counts, ht, haemoglobin (hb, total and free).
Primary separation was more efficient in the Optipress II because the platelet count was lower in the erythrocyte concentrates ( P  < 0.0001), platelets were lower in plasma ( P  < 0.0001) and platelet counts were higher in buffy coats ( P  < 0.0001). Buffy coat volume showed less variation (Optipress II VC = 4%, Optipress I VC = 7.4%). Secondary separation did not show differences between the Optipress II and manual method but was advantageous because of the automatic termination of the procedure.
Further improvement of standardization in blood component preparation is possible with an automated blood processor, leading to improvement of the quality of blood products for patient care.     

Shaping ability of the M4 handpiece and Safety Hedstrom Files in simulated root canals

The aim of this study was to assess the shaping ability of the M4 reciprocating handpiece and Safety Hedstrom files in simulated canals. A total of 40 simulated canals of various angles and positions of curvature were prepared with an M4 handpiece using Safety Hedstrom files oriented with the ground, flattened surface towards the inner aspect of the curve. A standard regimen was adopted throughout. Pre- and post-operative longitudinal images of the canals were taken with a video camera and stored and manipulated in a computer with image analysis software. The presence of canal aberrations and the amount and location of resin material removed as a result of preparation were determined from composite images of superimposed pre- and post-operative views. Preparation time varied significantly (P<0.001) between the canal types; overall, 20° canals were prepared more quickly than 40° canals. Zips and elbows were observed in 16 out of the 40 canals with most (11) being created in 40° specimens. Ledges were found in 19 canals and perforations in only 1. There were no significant differences between canal shapes for these aberrations. Excessive removal of material from the inner aspect of the canal at the curve to create a danger zone was found in 20 canals, but only in those with 40° curves. Significant differences in total canal width between the canal types were seen at the zips (P<0.05), elbows (P<0.05) and danger zones (P<0.001). Transportation at the danger zones varied significantly (P<0.001) between canal types. Under the conditions of this study, the M4 handpiece and Safety Hedstrom files created hour-glass preparations in a substantial proportion of canals. In reality, the Safety Hedstrom file with its one flattened surface was ineffective at reducing removal of material along the inner aspect of canal curves in severely curved specimens and clearly has the potential to create strip perforations in teeth.     

SCP对人乳腺癌细胞增殖抑制作用的研究

目的：研究SCP对人乳腺癌细胞生长，抑制效应，探讨其抗癌作用机制。方法：用不同浓度的SCP加入体外培养的人乳腺癌细胞（MCF－7）中，观察加药后细胞生长和有丝分裂指数（MI）的变化，用MTT法检测其细胞毒作用；Hoechst33342/PI荧光双染色方法检测细胞凋亡。结果：SCP明显抑制MCF－7细胞生长，IC50值为1mg/ml;MI于加药后48h较对照组明显降低；凋亡细胞比例在用药后48h达63．3％，结论：SCP可有效抑制人乳腺癌细胞的增殖。     

Movement-related potential measures of different modes of movement selection in Parkinson's disease

Movement-related potentials were recorded preceding self-paced voluntary movements in patients with Parkinson's disease and in healthy subjects of the same age group. We compared the Readiness Potential preceding joystick movements in a fixed direction and preceding joystick movements in freely selected directions. In normal subjects the Readiness Potential amplitude was higher preceding freely selected movements than preceding movements in a fixed direction. The Readiness Potential in Parkinson patients failed to be modified by the different modes of movement selection. The modulation of the Readiness Potential by different ways of preparing for movement might be due to the supplementary motor area (SMA) being more strongly engaged by tasks requiring internal control of movements than by tasks that are externally structured. The results suggest that this task-dependent variation of SMA activity is reduced in Parkinson's disease. A failing capacity to adapt SMA activity to different task demands has previously been suggested by evidence from positron emission tomography studies using similar tasks.     

肾上腺皮质腺癌的诊断与治疗

目的 探讨肾上腺皮质腺癌的早期诊断、手术及围手术期处理。方法 回顾分析了1990年至2001年间31例肾上腺皮质腺癌临床资料．并与1978年至1989年同种病例作比较。结果 近10年患者病理分期更趋向早期，更易行根治性切除，5年的存活率明显提高。结论 影像学的发展，使患者易早期诊断；围手术期的充分准备是减少手术并发症及死亡率的关键。     

2种磨牙根管预备方法的成本-效果分析

目的:比较不锈钢K锉常规根管预备(SSPT)法与镍钛机用根管器械逐步深入预备(NRPT)法在磨牙根管治疗中的近期临床效果和成本/效果(C/E)值。方法:入选患者随机分为SSPT法(49例)和NRPT法(45例)2组,记录治疗后不良反应、补救治疗措施和所花费用,评价近期疗效并进行成本-效果分析,用SPSS11.5统计分析软件进行数据统计。结果:NRPT组治疗满意率优于SSPT组(P=0.034),但前者人均治疗成本显著高于后者(P=0.001)。对重度弯曲根管,NRPT组治疗满意率优于SSPT组(P=0.007);前者的C/E值较后者低394.52元;轻中度弯曲根管前者的C/E值较后者高192.04元。根尖正常患者,NRPT组治疗满意率高于SSPT(P=0.012),但前者C/E值比后者高32.32元;而根尖阴影患者NRPT组的C/E值较SSPT组高376.47元。结论:NRPT法的疗效优于SSPT法,但治疗成本较高;对重度弯曲根管患牙,NRPT法可使患者受益;对中轻度弯曲根管患牙,应选用SSPT法。对根尖正常的患者,NRPT法近期疗效更高,而治疗前有根尖病变的患者,SSPT法可节约治疗成本。     

微波干燥法对腊叶标本压制的实验研究

目的：寻求一种不受气候条件影响而能成功制作腊叶标本的新技术。方法：采用微波干燥法，即用微波炉，由创制的直径31cm瓦楞纸板、吸水纸与玻璃皿组成的压制程序进行微波干燥。结果：微波干燥法可用于腊叶标本压制，且优于传统的常规腊叶标本压制方法。结论：本研究为腊叶标本的制作提供了一个新方法，收到了便捷、高效、且保色的理想效果。