HPLC法测定丹栀逍遥片中芍药苷的含量

目的建立丹栀逍遥片中芍药苷的含量测定方法。方法采用高效液相色谱法。色谱柱:Kromasil C18柱(200mm×4.6mm,5μm);流动相:甲醇-水(用磷酸调节pH值至3.5)(13∶87);流速:1.0mL.min-1;柱温:室温;检测波长:230nm。结果芍药苷在0.28～1.76μg范围内线性关系良好,相关系数r=0.998 5,平均加样回收率为98.6%(RSD=2.2%)。结论该方法快速简便,结果准确,可用于其质量控制。     

不同贮藏年限白芍的化学成分分析

目的比较不同贮藏年限白芍的化合物含量变化。方法采用HPLC法,测定不同贮藏年限的白芍中芍药苷、芍药苷亚硫酸酯、芍药内酯苷和没食子酸的含量。结果芍药苷、芍药苷亚硫酸酯的含量变化与贮藏时间长短无明显相关性,芍药内酯苷含量随贮藏时间的延长存在降低的趋势,没食子酸含量变化不明显。结论白芍可贮藏的年限较长,但采用硫磺熏蒸会大大降低芍药苷的含量。     

Paeoniflorin inhibits the histaminergic system and promotes non-rapid eye movement sleep via adenosine A1 receptors

Insomnia is a common disorder seen in nearly 30%-35% of the general population worldwide,with a range of 10%-15% being assessed as moderate to severe disorders.Symptoms of insomnia include poor sleep quality,difficulty in falling asleep,frequent awakenings during the night and early morning awakenings.Insomnia give rise to fatigue,irritability,impaired concentration and reduced alertness,causing serious impact on the quality of life of affected individuals.Current pharmacological treatment of insomnia involves the use of sedative-hypnotic benzodiazepines(BZ) and non-benzodiazepine drugs(Z-drugs).Z-drugs are the first line of management followed by BZ,amitryptiline and antihistamines.Insomnia could be effectively treated by the Z-drugs,such as zolpidem and the BZ,but their multiple adverse effects hamper their application.Paeoniflorin {PF,5beta-[(Benzoyloxy)methyl]tetrahydro-5-hydroxy-2-methyl-2,5-methano-1H-3,4-dioxacyclobutapentalen-1alpha(2H)-yl-beta-D-glucopyranoside,C23H28O11} is one of the principal active ingredients of Paeonia Radix.PF possesses inhibitory effects in the nervous system,such as suppress spike discharges and analgesic effect.These observations indicate that PF might have a potent sleep promoting effect.The aim of this study was to determine whether PF could modulate sleep behaviors by recording EEG and electromyogram in mice.The results showed that PF 25 and 50 mg·kg-1 could significantly shorten the sleep latency;increase the amount of non-rapid eye movement(non-REM,NREM) for 3 h,with an increase in the number of NREM and REM sleep episodes.PF 25 and 50 mg·kg-1 increased the number of bouts of wakefulness but decreased their duration.On the other hand,PF increased the number of state transitions from wakefulness to NREM sleep and subsequently from NREM sleep to wakefulness.However,PF had no effect on the amount of REM sleep.Immunohistochemical study showed that PF increased c-Fos expression in the neurons of ventrolateral preoptic area(VLPO),a sleep center in the anterior hypothalamus,and decreased c-Fos expression in the arousal tuberomammillary nucleus(TMN),which was located in the caudolateral hypothalamus.The sleep-promoting effects and changes in c-fos induced by PF were reversed by CPT,an antagonist at the adenosine A1R.We found a high degree of colocalization between histaminergic and gabaergic neurons in GAD 67-GFP mice TMN.Then we use GAD 67-GFP mice to help indentify histaminergic neurons.PF inhibited histaminergic neurons in the TMN by whole-cell patch clamping in the brain slice,CPT totally blocked the histaminergic neurons inactivation induced by PF.These results indicate that PF increased NREM and REM sleep by inhibiting the histaminergic system via A1R,suggesting its potential application for the treatment of insomnia.     

高效液相色谱法测定复光颗粒中芍药苷含量

目的 建立复光颗粒中芍药苷的含量测定方法.方法 采用高效液相色谱法,流动相为甲醇-0.05 mol/L磷酸二氢钾溶液(35:65),检测波长为230 nm,柱温为30℃.结果 芍药苷质量浓度线性范围为0.075～40 g/L,平均回收率为98.66%,RSD＝0.62%(n＝6).结论 该方法可靠、简单可行,为控制复光颗粒的内在质量提供了科学依据.     

基于5组分测定和6波长高效液相色谱指纹谱的双标定量指纹法建立六味地黄丸对照指纹图谱动态技术标准研究

目的 建立双标定量指纹法精确恢复对照指纹图谱(RFP)理论和方法,实现动态指纹图谱标准的校正和复原.方法 用RP-HPLC法对LWDHW中5组分进行定量分析和建立其6波长定量指纹图谱研究.结果 利用HPLC-DAD在最大吸收波长处同时测定了没食子酸、5-羟甲基糠醛和丹皮酚含量,结合等吸收双波长消去法对色谱峰完全重叠的芍药苷和马钱苷进行同时定量测定,建立210、230、246、265、280和310 nm LWDHW-HPLC指纹谱,用均值法整合6波长HPLC定量指纹谱.以5组分测定和6波长指纹谱鉴定结果分别聚类分析比较不同厂家产品质量差异,结果以后者更为准确可靠,在此基础上建立LWDHW双标定量指纹法技术标准.结论 基于多指标定量分析和平行多波长高效液相色谱指纹谱的双标定量指纹法能够找到产生RFP的标准制剂和实现实时随行定量检测LWDHW指纹图谱.这一方法再现了中药指纹图谱标准,为中药指纹图谱工业化整体定量控制中药质量建立了可行技术方法,是中药指纹图谱评价方法发展历程中的一次技术飞跃.     

芍药苷药理作用研究新进展

目的 总结芍药苷药理作用的研究现状,为进一步研究芍药苷提供文献依据和研究思路.方法 对近10年芍药苷相关文献进行整理、分析、归纳.结果与结论 芍药苷具有多种生物活性,对心血管、中枢神经系统免疫、以及平滑肌等方面均有肯定的药理作用.现代药理研究为芍药苷的临床疗效提供了新的理论依据和开拓了更为广泛的临床应用范围.     

反相高效液相色谱法测定附桂骨痛颗粒中芍药苷的质量比

目的对附桂骨痛颗粒中有效成分芍药苷的质量比测定方法进行研究。方法采用反相高效液相色谱（RP—HPLC）法测定附桂骨痛颗粒中芍药苷的质量比。结果测得附桂骨痛颗粒中芍药苷质量比为3．301mg／5go结论采用RP—HPLC法测定附桂骨痛颗粒中挣药苷的质量比可作为附桂骨痛颗粒的定量标准,测定方法简便、准确、可靠、重现性好。     

胃炎颗粒质量标准研究

目的建立胃炎颗粒中芍药苷的含量测定方法。方法采用反相高效液相色谱（RP—HPLC）法,色谱柱为C18柱（250mm×4．6mm,5μm）,流动相为乙腈-水（19：81）,检测波长229nm,流速1．0mL／min,柱温30℃,进样量10μL。结果芍药苷质量浓度在2．45～12．24μg／mL范围内与峰面积线性关系良好,平均回收率为99．80％,RSD=0．99％（n=9）。结论RP—HPLC法操作简单,检测结果准确可靠,可用于胃炎颗粒的质量控制。     

三痹消痛酒质量控制研究

目的控制医院制剂三痹消痛酒的质量,确保临床疗效。方法用薄层色谱（TLC）法鉴别白芍和三七,用高效液相色谱（HPLC）法测定芍药苷的含量。结果TLC法能明显检出白芍和三七;芍药苷进样量在0．325—0．975μg范围内与峰面积线性关系良好,回归方程为Y=1285 667．905X＋5122.2621,r=0．99999（n=5）,芍药苷的平均回收率为99．08％,RSD=0．47％（n=6）。结论所用定性定量方法简便、准确,重现性好,能较好控制产品质量。     

清热利湿颗粒提取工艺的实验研究

目的优选清热利湿颗粒的最佳提取工艺。方法水提取工艺以黄柏单煎,采用正交设计法以浸膏得率和盐酸小檗碱含量为考察指标;赤芍等8味药材合煎,采用正交设计法以浸膏得率和芍药苷含量为考察指标,优选最佳提取工艺条件。结果清热利湿颗粒最佳工艺条件：黄柏单煎加入10倍量水,煎煮2次,每次1.5 h;赤芍等药材加入10倍量水,煎煮2次,每次2小时。结论优选出的提取条件科学合理,提取工艺经验证,重现性较好,有效成分转移率较高。