Effect of ovulation on haem metabolism in rabbits

To investigate the origin of the cyclic changes in the rate of endogenous carbon-monoxide production (nCO) during the menstrual cycle, haem turnover was determined before and after chorion gonadotropic hormone-induced ovulation in six female rabbits. 14C-labelled delta-aminolevulinic acid and glycine were administered and the excretion rate of 14CO (A14CO) was measured for determination of hepatic and bone-marrow haem turnover, respectively. Carbon-monoxide production (nCO) was measured to estimate total haem turnover. After ovulation A14CO was increased significantly the first 2 h of the early labelled peak after 14C-ALA administration and was increased also at the first determination during the early peak after 14C-glycine but statistically not significantly. The total excretion of labelled CO during the period of the early labelled peak was not increased with any of these precursors for haem synthesis. On the other hand nCO was increased 34% (P less than 0.05) during the post-ovulation period. As the increase in 'unassigned' haem turnover was small and may be unaccompanied by a contemporary increase in bilirubin/CO production, it was concluded that the increase in nCO during the post-ovulation period essentially depends on increased destruction of circulating red cells in the rabbit.     

B超及宫颈评分预测排卵及其临床应用

用B 超对21不孕妇女的47个排卵周期进行观察,结合宫颈评分预测排卵并应用于临床指导用药,受孕率57.1%。本文观察卵泡相互垂直的三个最大直径的平均值发现卵泡生长速度有2个加速期:卵泡成熟期与排卵前期。对可能发生未破裂卵泡黄体化者,结合B 超及宫颈评分适时肌注hCG 5000 IU 促排卵,成功率100%,并对排卵前后超声显象进行了探讨。     

Localization of immunoreactive alpha-bag-cell peptide in the central nervous system of Aplysia

The bag cells of the marine mollusc Aplysia are well-characterized neuroendocrine cells that initiate egg laying, but the natural stimulus triggering bag-cell activity has not been determined. As a first step toward identifying central neurons that might provide synaptic or neurohormonal input onto the bag-cell network, antibodies specific for alpha-bag-cell peptide (alpha-BCP) were generated. This peptide belongs to a small family of structurally related peptides that can elicit bag-cell activity in vitro. Antibody specificity was established by immunodot assay and preabsorption studies: immunocytochemical labeling was abolished in each ganglion when the antibodies were preincubated with either alpha-BCP-thyroglobulin conjugate or alpha-BCP-(1-8) but was not affected by preincubation with thyroglobulin or thyroglobulin-thyroglobulin conjugate. The antibodies specifically labeled the bag cells in the abdominal ganglion and ectopic bag cells in both the abdominal and right pleural ganglia. The ectopic bag cells were similar to conventional bag cells in size and morphology, but varied in number and location among preparations. In the cerebral ganglion, the antibodies labeled a bilaterally symmetrical pair of cell clusters, containing approximately ten cells each, on the dorsal surface of the ganglion. The cerebral cells were smaller than bag cells, were constant in location, and sent their processes into the neuropil rather than the connective tissue sheath. Immunoreactive processes were observed in the neuropils of the cerebral, pleural, and pedal ganglia and among the axons of the cerebropedal, cerebropleural, and pleurovisceral connectives. No immunoreactive cell bodies were observed in the buccal or pedal ganglia. Identical patterns of labeling were observed in Aplysia californica, A. brasiliana, and A. dactylomela. The distribution of immunoreactive cell bodies within the circumesophageal ganglia of all three species thus parallels the distribution of receptive sites for the in vitro induction of bag-cell activity by atrial gland peptide B, a peptide structurally related to alpha-BCP. These observations suggest that the immunoreactive cells identified in these studies, or a subset of them, may be involved in the physiological induction of bag-cell activity. Since low doses of alpha-BCP have additional inhibitory actions on the bag cells, however, it is possible that the identified cells could play a more complex role in the regulation of bag-cell activity.     

Programmed ovulation induction and oocyte retrieval for in vitro fertilization

Forty-two patients underwent programmed ovulation induction for oocyte retrieval. They were treated in the preceding cycles with a progestagen, ethynodiol diacetate, at a dose of 2 mg twice daily. Two groups were defined based upon the stimulation protocol: Group A1 was stimulated with clomiphene citrate and human menopausal gonadotropin (hMG), and Group A2 with follicle-stimulating hormone (FSH) and hMG. They were compared to two randomized control groups of patients who received the same induction but were classically monitored. There was a high proportion of spontaneous ovulations in the programmed group (8/42) compared to the nonprogrammed group (0/42). There was a nonsignificant difference in the number of oocytes obtained or embryos replaced per cycle. Four pregnancies were obtained in the programmed group (24% per transfer), against 10 in the nonprogrammed patients (32% per transfer). The results of this method seem to be better using FSH for ovulation stimulation and a verification of the serum estradiol on the day of induction with human chorionic gonadotropin (hCG) and the following day (semiprogrammed method).     

尿LH快速测定结合宫颈粘液评分预测排卵

对６０例无明显盆腔病变的不孕妇女，在预计排卵前２￣３ｄ开始测尿ＬＨ及宫颈粘液评分，连续４￣６次，其中２０例同时作血ＬＨ含量测定。结果显示：血ＬＨ峰值与尿ＬＨ阳性符合率为８１．９％；血ＬＨ无峰值与尿ＬＨ阴性符合率为８８．９％。宫颈粘液评分值与尿ＬＨ（＋）呈正相关，与尿ＬＨ（－）呈负相关。经ｕ检验，可以ＣＳ７分作为有无排卵的分界线，因此认为尿ＬＨ测定结合宫颈粘液评分，为预测排卵的有效简介方法。     

Correlation between sexual desire and menstrual cycle characteristics

An extensive set of prospective data was examined for evidence of covariation between the timing of sexual desire and a number of menstrual cycle characteristics. In any given menstrual cycle, sexual desire was usually first experienced a few days before the basal body temperature (BBT) shift, around the expected ovulation date. Furthermore, positive correlations were found between the day of the BBT shift and the day of sexual desire onset, and between the length of the menstrual cycle and the temporal lag between the onset of sexual desire and the BBT shift. These results are consistent with a model in which sexual desire is affected by the same process that regulates the menstrual cycle. This process is presumably hormonal in nature, suggesting that hormonal factors contribute to sexual desire.     

Serum prolactin profiles of normal human conceptual cycles

Commencing on day 10 of the menstrual cycle through onset of subsequent menses, or confirmation of pregnancy, daily sera collected from 15 women planning pregnancy were analyzed by radioimmunoassays (RIA) for prolactin (hPRL), estradiol-17β and luteinizing hormone (hLH). Two of the observed subjects became pregnant in the single cycles studied. The profiles of these hormones during the early gestation following spontaneous ovulation were established. No distinct midcycle peaks of hPRL were observed in either subject. Enormous spikes were observed in daily prolactin values, with wide variations between subjects.     

助黄汤促排卵作用的临床观察

我们用助黄汤治疗排卵障碍取得了良好的疗效,现报告如下。1资料与方法1.1病例选择夫妇婚后正常同居2年以上未避孕而未孕、月经规律或不规律、半年内未经激素治疗者;排除有心理障碍(忧郁、精神分裂症),常规妇科检查排除各种器质性病变(子宫肌腺症、子宫内膜异位症及输卵管疾病等)、炎症性病变、高泌乳素血症、高雄激素症,以及排除男方因素所致不孕。1.2一般资料1998年12月~2005年8月在龙华医院门诊就诊,年龄25~39岁的已婚妇女,因无排卵伴不孕59例,其中原发不孕23例,继发不孕36例。分为治疗组和对照组。治疗组32例,年龄最大37岁,最小24岁,平均…