尖锐湿疣患者人乳头瘤病毒的基因分型

目的 探索尖锐湿疣患者人乳头瘤病毒的基因分型。方法 PCR方法检测HPV6／11型、HPV16／18型DNA。结果 检测尖锐湿疣患者100例，对其中35例进行组织活检，阳性检出率为91．4％(32／35)，阳性检出中，HPV6／11型26例(81．2％)，HPV16／18型3例(9．4％)，HPV6／11 HPV16／18型3例(9．4％)；对其中65例进行宫颈抹片检测，阳性检出率为92．3％(60／65)，阳性检出中，HPV6／11型41例(68．3％)，HPV16／18型12例(20．0％)，HPV6／11 HPV16／18型7例(11．7％)。结论 尖锐湿疣患者人乳头瘤病毒感染以HPV6／11型为主，HPV16／18型在宫颈处感染率高于其它处组织。     

广东地区淋球菌、沙眼衣原体及解脲脲原体感染的分子流行病学调查

目的了解广东地区淋球菌(NG)、沙眼衣原体(CT)、解脲脲原体(UU)等性病的流行病学状况.方法对广东地区76家基层医院性病门诊就诊的 1 949 例生殖道感染患者取其分泌物,用PCR方法检测NG,CT,UU-DNA.结果 262例男性患者NG,CT,UU阳性检出率分别为7.63%,23.28%,10.69%;1 687 例女性患者NG,CT,UU阳性检出率分别为9.19%,17.90%,17.19%.男性CT感染率23.28%明显高于女性17.90%(P＜0.05),女性UU感染率17.19%高于男性10.69%(P＜0.05),男、女性NG阳性率分别为7.63%和9.19%差异无显著性(P＞0.05).NG+CT混合感染率为3.27%,NG+UU混合感染率为2.34%,CT+UU混合感染率为8.88%,NG+CT+UU混合感染率为0.93%.1999～2001年3种性病感染率分别为26.11%,43.81%和51.52%.结论广东地区NG,CT,UU 3种性病的流行较为严重,总感染率高达43.92%,且呈逐年增长的趋势.     

定量RT-PCR法对"苦参"在心肌炎模型中药效学研究

目的用定量RT-PCR法检测“苦参”中抗柯萨奇B病毒有效成份“抗柯注射液”,在Balb/c小鼠病毒性心肌炎模型病毒血症时,对抗“柯萨奇B病毒”的药效。 方法取48只雄性18-22g 8周龄的Balb/c小鼠,随机分成A-H共8组,每组6只。1.各治疗组(A-F组),每只小鼠从腹腔接种0.1ml l04TCID50 CVB3m病毒2h后,每天从尾静脉分别注射抗柯注射液,5、10、15、20、25、30mg/kg,2次/d,连续用药3d。2.病毒组(G组),注射病毒同上法,2h后从尾静脉注入0.3ml无菌生理盐水,2次/d,连续3d。3.空白对照组(H组),腹腔内注射无小牛血清的RPMI-1640培养液,2h后从尾静脉注入0.3ml无菌生理盐水,2次/d,连续3d。 结果 空白组CVB-RNA(-)对接种CVB3m感染后用不同剂量“抗柯注射液”治疗的各小组血液中CVB3m-RNA含量与病毒组相比明显下降,其抑制率(％)与所用抗柯注射液的剂量呈正比,即“抗柯”剂量增加,CVB3m-RNA含量下降的幅度亦增加,每天5-30mg,/kg中的各剂量都有明显抑制病毒增殖的作用。 结论用定量RT-PCR法检测到“抗柯注射液”对Balb/c小鼠病毒性心肌炎病毒血症时效果明显,最小有效剂量为5mg·kg-1·d-1。     

脑肿瘤中p53基因突变的分析

检测２５例脑肿瘤中抑制癌基因Ｐ５３突变情况。突用复合ＰＣＲ，聚合酶链反应－＝单链构象多态性分析法，统计学处理采用Ｘ＾２检验。结果：Ｐ５３基因总的突变率为４４．０％，其中第５外显子突变率为２８．０％，第６外显子突变率１２．０％，第７外显子突变率为１２．０％，第８外显子突变率１６．０％；病理分级Ⅱ级及其以上突变为７２．７％，     

Virological, biochemical and histological effects of human lymphoblastoid interferon in Swedish patients with chronic hepatitis C

Thirty-eight Swedish patients with chronic hepatitis C were randomly assigned to receive either 3 million units (MU) or 5MU of human lymphoblastoid interferon-α-n1 (Wellferon) three times per week for either 6 or 12 months. The patients were monitored biochemically, histologically and by quantitative polymerase chain reaction for circulating HCV RNA, during therapy and for the following year. Overall, 22 (58%) of the patients lost detectable hepatitis C virus (HCV) viraemia during therapy but eight of these patients relapsed during follow-up, leaving 14 (37%) sustained responders. Patients infected with HCV non-type 1 genotypes were significantly more likely to achieve a sustained response than were those infected with HCV type 1 (63% vs 10.5%, P =0.001). Sustained virological responses were also associated with lower pretreatment viraemia level, younger age, absence of cirrhosis and the higher interferon dosage regimens but these associations failed to reach statistical significance. In 97% of patients there was concordance between virological and biochemical responses, and a statistically significant ( P =0.005) improvement in the Knodell histological activity index was observed in the virological sustained responders.     

An atypical case of mononeuritis multiplex in primary Sjogren's syndrome

We present an atypical case of peripheral nervous system (PNS) involvement in Sjogren's syndrome in a 63 year-old woman. Symptoms of an entrapment neuropathy were the first manifestation of the systemic disease and they were subsequently coupled to those of a mononeuritis multiplex. Clinical and laboratory signs for the diagnosis of Sjogren's syndrome became subsequently overt. The mononeuritis multiplex remained clinically limited to the upper limbs and characterized by unusually severe motor symptoms which progressed up to the development of a final complete deplegia. By contrast, sensory symptoms at the upper limbs remained mild over the entire course of the disease and the lower limbs revealed a subclinical sensory-motor damage only during the late stage.     

PCR检测食蟹猴疟原虫的实验研究

目的 建立一种特异、敏感、简捷的PCR检测食蟹猴疟原虫（P.C）的方法。方法 检测样品来自人工接种感染P.C的阳性猴血膜及滤纸血斑。根据P.C　SSUrRNA基因序列,设计P.C特异引物Pc1和Pc3,用PCR常规操作对P.C DNA模板扩增。同时用多对间日疟原虫（P.V）特异引物对照比较。结果 从 P.C血样中扩增出 425bp特定扩增带,而P.V红内期和子孢子、恶性疟原虫、伯氏疟原虫、人基因组DNA和健康猴血均未见扩增带;敏感度可检测 1.68 ×10-6的原虫感染水平。同时发现P.C对多对 P.V特异引物均出现阳性扩增带,以往一直未被注意。结论 该方法检测 P.C特异、敏感和简捷,提供了鉴别 P.V与 P.C的准确诊断。建议在 PCR的P.V引物设计和特异性的检测过程,把应用P.C　DNA检测列为常规对照,以提高检测质量。     

浙江省在鼠中检测到埃立克体及无形体DNA片段并测序分析

目的:了解鼠中自然感染埃立克体及无形体的状况。方法:用巢式PCR扩增鼠中埃立克体属及嗜粒细胞无形体属16S rDNA的5′末端片段,对阳性产物克隆后测序。结果:在浙江省金华市金东区捕到鼠128只。在黄毛鼠中检到阳性4份,阳性率为3.125%,并对其进行克隆后测序。与Genbank上比对分析,三份与牛埃立克体相同基因区最为接近,属无形体科,无形体属。一份与反刍动物埃立克体相同基因区最为接近,属无形体科,埃立克体属。均为国内首次报道。结论:初步认为浙江省存在埃立克体及无形体病原。黄毛鼠可能为埃立克体及无形体宿主,尚需进一步调查。