HPLC-UV测定五更清肠溶片中补骨脂素的含量

目的建立五更清肠溶片中补骨脂素的含量测定方法。方法HypersilODS C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(45∶55),检测波长为246 nm。结果补骨脂素在12.5~125μg.m l-1范围内,浓度与峰面积值呈良好线性关系,r=0.9996,平均加样回收率为98.12%,RSD=1.8%。结论本方法简便,重复性好,可用于五更清肠溶片的质量控制。     

差示紫外分光光度法测定电光滴眼液中盐酸丁卡因的含量

目的建立差示分光光度法测定电光滴眼液中盐酸丁卡因的含量。方法在331 nm,以盐酸丁卡因的0.1 mo.lL-1盐酸溶液和水溶液中不同的紫外光谱,测得差示吸收值为定量依据。结果平均回收率为99.0%,RSD为0.59%(n=5)。结论方法简便,结果准确。     

2％盐酸丁卡因溶液的稳定性预测及影响因素考察

目的研究2%盐酸丁卡因溶液的稳定性及影响因素。方法采用初均速法测定其在20℃时、含量下降5%的贮存期;并将成品在避光与室内光、低温与常温条件下进行留样观察,定期测定含量。结果2%盐酸丁卡因溶液在20℃、含量下降5%时的贮存期为13个月。在避光条件下贮存,其含量下降较室内光缓慢;在低温(8℃±1℃)条件下贮存,其含量下降较常温缓慢。结论2%盐酸丁卡因溶液的有效期可定为一年,以低温、避光贮存为宜。     

盐酸甲氯芬酯治疗急性缺血性脑卒中疗效评价

目的评价盐酸甲氯芬酯治疗急性缺血性脑卒中的临床疗效和安全性。方法198例经头颅CT或MRI确诊为急性缺血性脑卒中,且美国国立卫生院神经功能缺损评分(NIHSS)为8~22分的患者被随机分为两组:盐酸甲氯芬酯组103例,对照组95例。盐酸甲氯芬酯组在对照组治疗用药基础上加用盐酸甲氯芬酯0.25g(加入0.9%氯化钠溶液250mL中)静脉滴注,一日1次,14天为一个疗程。结果治疗后第3、7、14、28天,盐酸甲氯芬酯组患者NIHSS明显降低而生活能力状态(ADL)评分明显提高,与对照组相比差异显著(P<0.05);治疗后第3、7、14、28天盐酸甲氯芬酯组的总有效率显著高于对照组(P<0.05);两组不良事件的发生率无显著差异(P>0.05)。结论盐酸甲氯芬酯能改善急性缺血性脑卒中患者的神经功能缺损,是一种治疗急性缺血性脑卒中安全而有效的药物。     

阿比多尔双层缓释片的研制及体外释药

目的研制阿比多尔双层缓释片并考察其体外释药特性。方法以两种不同处方作湿法制粒、压制双层片，紫外分光光度法测定阿比多尔的释放度。结果阿比多尔双层缓释片的释药曲线用Higuchi方程或Peppas方程拟合，可维持121、持续释放。结论研制的阿比多尔双层缓释片达到设计要求。     

减肥食品或保健品中非法添加盐酸西布曲明、盐酸芬氟拉明、酚酞的快速检测方法研究

目的：建立减肥食品或保健品中同时检测盐酸西布曲明、盐酸芬氟拉明、酚酞的薄层色谱方法。方法：样品经甲醇提取后，采用硅胶G薄层板，以甲苯-乙酸乙酯-异丙醇-浓氨试液（12：6：3：0．5）为展开剂，4％氢氧化钠溶液及稀碘化铋钾为显色剂。结果：在选定的色谱条件下，盐酸西布曲明、盐酸芬氟拉明、酚酞3种组分能得到较好的分离。结论：本法快速、简便、专属性强，可作为同时检测盐酸西布曲明、盐酸芬氟拉明、酚酞的快速检测方法。     

Physicochemical quality evaluation of natural compounds isolated from crude drugs

Aconite root has high toxicity caused by diester alkaloids, thus it was necessary to define the limiting value of diester alkaloids used in medicine formulation. To give the quality of “Processed Aconite Root” and “Powdered Processed Aconite Root” in the Japanese Pharmacopoeia (14th edn, supplement II), we established the official specification and evaluation methods of standard substances. High qualitative grade diester alkaloids, aconitine, hypaconitine, jesaconitine and mesaconitine, which were useful to evaluate the purity of processed aconite root and powdered processed aconite root, were prepared and evaluated for their stability. We studied the physicochemical specification and evaluation methods of these alkaloids. In addition, an “Aconitum diester alkaloids standard solution for purity”, which was used for the purity test, was prepared, and we also studied its physicochemical specification and evaluation methods. In addition, to evaluate the quality of processed aconite root and powdered processed aconite root, a TLC identification test was established. A monoester alkaloid of benzoylmesaconine hydrochloride was used as the reference standard in the latter test, and we also investigated its physicochemical specification and evaluation methods.