Detection of a human chromosomal translocation t(8;9) in a baby with multiple malformations using two-color fluorescence in situ hybridization

A human chromosomal translocation t(8;9) was detected using two-color fluorescence in situ hybridization with probes capable of staining the entire lengths of each of these chromosomes. The chromosome 8 probe was labeled with biotin and detected with Texas red, while the chromosome 9 probe was labeled with AAF and detected with FITC . In normal metaphase spreads, two metaphases from the proband, two red, one green and one part red and part green derivative chromosome were seen. The bicolor chromosome corresponded to translocation of a chromosome 8 segment to the distal part of the q region of one chromosome 9, as originally indicated by banding analysis. In interphase nuclei of the proband, four domains with bright fluorescence were recognized in many nuclei. Two were red, one was green, and the fourth had portions of both colors, indicating the presence of the translocation.     

氢化物原子荧光光度法测定食品中砷的不确定度评定

目的探讨氢化物原子荧光光度法测定食品中砷的不确定度评定方式。方法依据JJF1059-1999建立数学模型,合成不确定度。结果当食品中砷含量为0.22mg/kg时,合成不确定度为0.008mg/kg,扩展不确定度为0.02mg/kg。结论本评定过程清晰合理,主要影响因素不遗漏、不重复,适用于原子荧光光度法的不确定度评定。     

High-Performance Liquid Chromatographic (HPLC) Assay Using Fluorescence Detection for the Simultaneous Determination of Gallopamil and Norgallopamil in Human Plasma

Gallopamil is a calcium-channel antagonist with reported activity in experimental animals three to five times higher than that of verapamil. An automated high-performance liquid chromatographic (HPLC) method with fluorescence detection is described for the simultaneous determination of gallopamil and its metabolite norgallopamil in plasma. Gallopamil was well resolved from norgallopamil and other metabolites, allowing simultaneous quantitation of both drugs. The detection limit for both gallopamil and norgallopamil was 0.9 ng/ml. This method has been successfully used for the determination of gallopamil and norgallopamil following the administration of 25-, 37.5-, and 50-mg oral doses of drug.     

Drug-Biomacromolecule Interaction XII: Comparative binding study of sulfaethidole to bovine serum albumin by equilibrium dialysis,fluorescence probe technique,uv difference spectrophotometry and circular dichroism

Binding of sulfaethidole to bovine serum albumin was studied by equilibrium dialysis, fluorescence probe technique, uv difference spectrophotometry and circular dichroism. Equilibrium dialysis method enabled us to estimate the total number of drug binding sites of albumin molecule. For sulfaethidole, albumin had 6 primary and 40 secondary binding sites. The primary and secondary binding constants were 0.9×10⁵ M⁻¹ and 0.2×10⁶ M⁻¹, respectively. 1-Anilino-8-naphthalenesulfonate (ANS) and 2-(4′-hydroxylbenzeneazo)-benzoic acid (HBAB) were used as the fluorescence probe and the uv spectrophotometric probe, respectively. In fluorescence probe technique, results indicated that the number of higher affinity drug binding site of albumin was 1 and the number of lower affinity drug binding sites of albumin was 3, and the primary and secondary drug binding constants for bovine serum albumin were 2.15×10⁵ M⁻¹ and 1.04×10⁵ M⁻¹, respectively. In uv difference spectrophotometry, binding sites were 3 and binding constant was 1.88×10⁵ M⁻¹. The above results suggest that several different methods should be used in ompensation for insufficient information about drug binding to albumin molecule given by only one method.     

Microstructure of the non-rapid eye movement sleep electroencephalogram in patients with newly diagnosed Parkinson's disease: effects of dopaminergic treatment.

We investigated non-rapid eye movement (non-REM) sleep in patients with newly diagnosed Parkinson's disease (PD) who had never previously received dopaminergic medication. There were no significant differences in the conventional sleep parameters between de novo patients with PD and a healthy control group, but the length of stage 1 sleep and the number of awakenings increased significantly upon administration of dopaminergic drugs. Analyzing the quantitative electroencephalogram (EEG), we observed a significant reduction in the low-delta frequency range and a nonsignificant increase in the sigma frequency range in de novo patients with PD. The dopaminergic medication also nonsignificantly reduced the low-delta and sigma frequencies, the latter to the level of the controls. Possible mechanisms that may account for the observed differences are discussed. It is suggested that Parkinson's disease as well as the application of dopaminergic drugs exerts a desynchronizing effect on the sleep EEG that is reflected in a disruption of sleep continuity.     

应用激光扫描共聚焦显微镜观察晶体上皮细胞中的游离钙

目的用钙的荧光指示剂ｆｌｕｏ－３和激光扫描共聚焦显微镜（ｌａｓｅｒｓｃａｎｎｉｎｇｃｏｎｆｏｃａｌｍｉｃｒｏｓｃｏｐｙ，ＬＳＣＭ）观察大白鼠的晶体上皮细胞（ｌｅｎｓｅｐｉｔｈｅｌｉａｌｃｅｌ，ＬＥＣ）中的游离钙。方法ｆｌｕｏ－３着染细胞后，应用ＬＳＣＭ观察细胞内ｆｌｕｏ－３与钙螯合后的荧光分布，最后用钙离子载体Ａ２３１８７和重金属离子Ｍｎ＋＋作校正，将ｆｌｕｏ－３与钙结合显示的荧光强度转换为［Ｃａ＋＋］ｉ值。结果ＬＥＣ中的游离钙主要分布在细胞核中。大白鼠晶体上皮细胞中的［Ｃａ＋＋］ｉ为２５９．７９±４９．２４ｎｍｏｌ／Ｌ。结论ｆｌｕｏ－３与ＬＥＣ孵育后能着染细胞。用ＬＳＣＭ能直观地观察细胞内钙的分布，并能通过校正得到细胞内游离钙的绝对值。这种成熟的方法，为进一步对ＬＥＣ中游离Ｃａ＋＋的研究提供技术准备     

Monoclonal antibodies to fungi of significance to the quality of foods and feeds

Monoclonal antibodies (MAbs) were raised against Penicillium aurantiogriseum var. melanoconidium. Cross‐reactivity studies were carried out against 12 ‘field’ and 27 ‘storage’ fungi. Two MAbs (MAbs 32 and 37) reacted with all of the fungi tested and a third (MAb 38) with 38 out of the 39 fungi (although weakly with some). Monoclonal antibodies 32 and 37, but not MAb 38, were found to react to a degree with ‘clean’ barley. Enzyme‐linked immunosorbent assay (ELISA) using MAb 38 and extracts of barley inoculated with the homologous antigen showed a clear relationship between absorbance and amount of fungal growth. It is suggested that these MAbs could be used in a broad‐spectrum assay to detect fungi of significance to the quality of foods and feeds.     

荧光光谱法分析煤焦油组分

研究煤焦油组分时,为了防止组分相互之间的干扰,在测定前用几种不同极性的溶剂:石油醚;80%石油醚-20%甲苯;60%石油醚-40%甲苯;40%石滑醚-60%甲苯;20%石油醚-80%甲苯;甲苯;氯仿;50%氟仿-50%甲醇;甲醇进行洗提,从煤焦油中得到9个级分。然后用荧光法从9个级分中鉴定出28个组分:茶、苊、联苯、三甲基酚、菲、异喹啉、芴、对氨基酚、联苯胺、蒽醌、四氢萘、2,5-二甲酚、蒽、咔唑、间苯二酚、二乙基苯胺、喹啉、吲哚醌、1,3,5-三甲苯、1,2,4-三甲苯、萘醌、菲醌、窟、苾、氧芴、1,2-苯并蒽、2,3-苯并蒽、3,4-苯并苾。同时定量了氯仿和甲醇级分中氧芴、(艹屈)、联苯胺、苾、菲、蒽、咔唑、四氢蔡咔、四氢萘、苯醌、喹啉、8-羟基喹啉等12个组分的含量。     

建立一种粪便细菌学检验的新方法--肠道菌谱分析(初步报告)

目的 建立一种以粪便标本细菌菌谱分析为主要内容的细菌学检验方法。方法 取粪便标本直接涂片做革兰染色，镜检观察标本中细菌数量、种类及比例，并进行显微照相；对粪便标本进行菌群培养，分离病原菌，并与镜检结果进行对照；将照片进行分类。建立菌群失调和病原菌感染的图谱。同时以正常大便菌谱作对照。结果 因菌群失调导致的腹泻。直接镜检表现为细菌数量和种类普遍减少，或某一类细菌增多或减少，或呈单一种类细菌；由某种病原菌引起的腹泻，直接涂片多数只能见到革兰阴性杆菌，且细菌数量少或极少，一般均与培养结果相符。结论 粪便细菌菌谱分析能直接或间接为临床提供腹泻患者的肠道微生态资料，尤其能准确地提示因各种原因造成菌群失调而导致的腹泻，为临床诊断和制定治疗方案提供快捷、科学的依据。     

二维多普勒超声对室性早搏的血流频谱分析

观察室性早搏４３例，多普勒频谱表现为峰值流速减慢，早搏使前一组Ａ峰消失，后一组峰值流速增快。１９例早发型，主动脉瓣口、肺动脉瓣口无频谱出现，早搏每搏量因发生时间不同，每搏量减少不同，晚发型平均减少３７．６％，早发型平均减少７９．６％。讨论了产生机理及对心排量的影响。