恶性疟原虫海南株线粒体复合体Ⅱ辅基铁-硫蛋白基因克隆及序列分析

目的：构建恶性疟原虫海南株线粒体复合体Ⅱ琥珀酸-泛醌还原酶辅基铁-硫蛋白(iron-sulfur protein,Ip)基因原核表达质粒pET28α-Ip，测定Ip基因序列，为研究铁-硫蛋白基因功能奠定基础。方法：采用PCR技术从恶性疟原虫海南株基因组DNA中扩增出Ip基因，扩增产物经纯化后，用BamHI XhoI双酶切，定向克隆入pET28α质粒，转化大肠杆菌DH5α，再用BamHI XhoI酶切及PCR扩增对重组子进行鉴定。用Sanger双脱氧链终止法进行DNA序列测定，并进行同源性比较。结果：筛选出编码海南株Ip基因的原核表达质粒pET28α-Ip，海南株Ip基因序列与K1、3D7株高度同源。结论：pET28α-Ip重组质粒的构建，为进一步研究铁-硫蛋白基因奠定基础。     

多组分单体熔融接枝聚丙烯及其性能研究

利用单螺杆挤出机研究聚丙烯的多组分极性单体熔融接枝，从而改善和提高聚丙烯的极性。红外和熔体流动速率的结果表明，对于单组分接枝体系，在适当的反应条件下，甲基丙烯酸缩水甘油酯、甲基丙烯酸羟乙酯等极性单体均可以接枝在聚丙烯上，但同时也伴随着较严重的聚丙烯降解。而采用多组分单体接枝体系，通过加入甲基丙烯酸缩水甘油酯和苯乙烯单体，或甲基丙烯酸羟乙酯和苯乙烯单体，能够有效地控制聚丙烯的降解，大幅度提高接枝率，     

莫匹罗星软膏与红霉素软膏治疗感染性皮肤病

将莫匹罗星软膏与红霉素软膏对照治疗感染性皮肤病，结果显示莫匹罗星软膏的疗效较红霉素软膏明显增高，经统计学分析治愈率（Ｐ＜０．０１）和总有效率（Ｐ＜０．０５）差异有显著性。该药不仅疗效高，而且不污染衣物，对皮肤也无刺激性，认为该药可作为局部抗感染的首选药物     

Acute effects of intravenous glucocorticoid pretreatment on the in vitro peroxidation of cat spinal cord tissue

The effects of a large intravenous dose of the glucocorticoid methylprednisolone on lipid peroxide formation in cat lumbar spinal cord homogenate was examined using the thiobarbituric acid test for malonyldialdehyde production. One hour after the injection of a 30-mg/kg dose of methylprednisolone, lipid peroxidation was reduced by more than a third. After a 90-mg/kg glucocorticoid dose, an opposite effect was observed; the formation of malonyldialdehyde was actually increased by more than 55%. These data demonstrate that glucocorticoid may either decrease or increase central nervous system lipid peroxidation as a function of dose. These results have possible clinical neurological implications.     

Inactivation and binding of human plasma kallikrein by antithrombin III and heparin.

Purified human antithrombin III was found to inactivate the esterase, the kininogenase, and the plasminogen activator activity of purified plasma kallikrein. The reaction rate was increased by heparin. Kinetic studies indicated second-order kinetics for the reaction between kallikrein and antithrombin III, and a catalytic effect of heparin. SDS polyacrylamide gel electrophoresis revealed that three new bands were generated during the inactivation of kallikrein by antithrombin III. Heparin did not influence the factor XII-dependent activation of prekallikrein in human plasma, whereas the inactivation of kallikrein elapsed slightly more rapidly in plasma samples where heparin was added. It is concluded that antithrombin III probably is of minor importance for the inactivation of kallikrein in plasma under physiological circumstances.     

常见球菌红霉素耐药基因研究

目的 探讨常见球菌红霉素耐药基因存在状况。方法 对常见细菌国内分离株进行红霉素耐药相关的红霉素核糖体甲基化酶基因（ermA／B／C／G／Q、errnTiB、crmF）、主动外排转运基因（mefA）检测。结果 肺炎链球菌、MRSA、MRCNS、粪肠球菌、屎肠球菌的crm基因检出率较高，其中肺炎链球菌并可检出mefA基因。结论 常见球菌国内分高株红霉素耐药的常见机制为携带红霉素梭糖体甲基化酶基因。     

Inhibition by nitroso-chloramphenicol of the proton translocation in mitochondria

We have found recently that, unlike chloramphenicol (CAP), its nitroreduction derivative nitroso-chloramphenicol (NO-CAP) behaved as a potent inhibitor of the energy-conserving mechanism in mitochondria [Abou-Khalil et al. Biochem. Pharmac.29, 2605 (1980)]. Concentrations of 75 and 250 μM NO-CAP were required to inhibit ATP formation with glutamate and succinate, respectively, whereas similar CAP concentrations were without effect. Testing several key reactions associated with the biosynthesis of ATP, inhibitory concentrations of NO-CAP were found to interfere as follows: (a) the transport of an NAD-linked substrate (e.g. glutamate) into mitochondria was only partially inhibited, whereas that of an FAD-linked substrate (e.g. succinate) was not inhibited but was rather slightly activated; (b) the transport of P_i was only inhibited at about 50%; (c) mitochondrial ADP transport was not affected at all; (d) the ATPase activity, measured either by Pi release in the presence of an uncoupler or by H⁺ ejection, was only slightly affected; and (e) under either phosphorylation or no phosphorylation conditions and in the absence of P_i, NO-CAP was found to completely block mitochondrial H⁺ extrusion resulting from the oxidation of either succinate or glutamate; however, under similar conditions the oxidation of the two substrates was not totally inhibited. The possibility of interference by NO-CAP with reactive mitochondrial thiols groups is discussed in the light of previous data and current experiments showing protection by P_i against NO-CAP effects on respiration. Moreover, NO-CAP as compared to conventional inhibitors of oxidative phosphorylation (e.g. rotenone, antimycin A, oligomycin, mersalyl and others) appeared to have a distinct mode of action on that process. The results demonstrate that the inhibitory effect of NO-CAP is primarily located at the respiratory chain level where the proton translocation activity is fully blocked.     

SPECT脑血流灌注显像定量测定正常人rCBF

目的 建立一种简便、实用、可行、便于临床应用的rCBF定量测定方法。方法 10例正常人经严格筛选静脉一次性弹丸注射^99Tc—ECD行放射性核素血管显影动态采集，10分钟后行SPECT脑断层采集。然后依据动态图象勾画主动脉弓及大脑感兴趣区，根据公式得到脑灌注系数(BPI)和脑平均血流量(mCBF)。处理断层图象结合Lassen校正得到大脑局部脑血流量(rCBF)。结果 本方法所测正常人(10例)的平均脑血流量(mCBF)以及局部脑血流量(rCBF)同其他经典方法(PET ^150-H2O法，SPECT ^123I-IMP法)所测mCBF和rCBF文献值具有很好的一致性。结论 本方法是一种原理可靠，操作简单，易于推广的CBF测定方法，具有较高的临床应用价值。