全文获取类型
收费全文 | 173290篇 |
免费 | 13551篇 |
国内免费 | 6756篇 |
专业分类
耳鼻咽喉 | 1260篇 |
儿科学 | 2926篇 |
妇产科学 | 2567篇 |
基础医学 | 18886篇 |
口腔科学 | 3616篇 |
临床医学 | 16645篇 |
内科学 | 24412篇 |
皮肤病学 | 2727篇 |
神经病学 | 10818篇 |
特种医学 | 4329篇 |
外国民族医学 | 41篇 |
外科学 | 15288篇 |
综合类 | 28715篇 |
现状与发展 | 20篇 |
预防医学 | 12888篇 |
眼科学 | 3606篇 |
药学 | 20813篇 |
46篇 | |
中国医学 | 7937篇 |
肿瘤学 | 16057篇 |
出版年
2024年 | 273篇 |
2023年 | 1864篇 |
2022年 | 3781篇 |
2021年 | 5723篇 |
2020年 | 4963篇 |
2019年 | 6790篇 |
2018年 | 6366篇 |
2017年 | 5863篇 |
2016年 | 5652篇 |
2015年 | 5825篇 |
2014年 | 11259篇 |
2013年 | 12268篇 |
2012年 | 11135篇 |
2011年 | 11845篇 |
2010年 | 10080篇 |
2009年 | 10029篇 |
2008年 | 9363篇 |
2007年 | 9927篇 |
2006年 | 8914篇 |
2005年 | 7618篇 |
2004年 | 5740篇 |
2003年 | 5195篇 |
2002年 | 4226篇 |
2001年 | 3727篇 |
2000年 | 3116篇 |
1999年 | 2659篇 |
1998年 | 2329篇 |
1997年 | 1989篇 |
1996年 | 1795篇 |
1995年 | 1650篇 |
1994年 | 1374篇 |
1993年 | 1047篇 |
1992年 | 933篇 |
1991年 | 771篇 |
1990年 | 693篇 |
1989年 | 554篇 |
1988年 | 465篇 |
1987年 | 424篇 |
1986年 | 374篇 |
1985年 | 823篇 |
1984年 | 835篇 |
1983年 | 573篇 |
1982年 | 622篇 |
1981年 | 447篇 |
1980年 | 467篇 |
1979年 | 325篇 |
1978年 | 202篇 |
1977年 | 173篇 |
1976年 | 150篇 |
1975年 | 118篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
目的分析探讨影响注意缺陷多动障碍(ADHD)患儿延迟就诊的因素。方法采用家庭环境量表(FES-CV)及自编简明就诊因素问卷对68例ADHD患儿的家长进行调查评定,分析影响延迟就诊的相关因素。结果61例患儿完成调查。所有因素经多元线性逐步回归分析,3个自变量差异具有统计学意义,分别为分型(Beta值:-0.449),接触知识(Beta值:0.369),父亲文化(Beta值:-0.268)。结论影响患儿就诊时间延迟的因素有3种情况,其顺序如下:(1)ADHD的注意缺陷型;(2)家长缺乏儿童心理卫重知识;(3)父亲的文化程度低。 相似文献
992.
酞丁安对映体合成及其抗单纯疱疹病毒活性评价 总被引:1,自引:0,他引:1
酞丁安(3-酞酰亚胺-2-氧-正丁醛双缩氨硫脲,TDA)是药物研究所创制的抗病毒新药。为了研究其对映异构体(R),(S)-TDA对病毒活性及毒性是否有差异,并与消旋酞丁安(RS)-TDA的抗病毒活性及毒性进行比较,本文分别用已知构型的(R)-与(S)-丙氨酸为原料,通过缩合等6步反应,得到光学活性的(R)-,(S)-TDA,并与外消旋酞丁安比较其抗病毒活性及毒性。三者的抗单纯疱疹病毒活性与对细胞的毒性差别不大,说明消旋酞丁安临床使用是安全有效的。 相似文献
993.
RICHARD G. LEA JENNY UNDERWOOD KATHY C. FLANDERS HAL HIRTE DALJEET BANWATT SUZETTA FINOTTO ISAO OHNO SALIM DAYA CALVIN HARLEY MAGDY MICHEL JAMES F. MOWBRAY DAVID A. CLARK 《American journal of reproductive immunology (New York, N.Y. : 1989)》1995,34(1):52-64
PROBLEM : To determine if patients with unexplained recurrent miscarriage have a deficiency of decidual immunosuppressor cells that produce transforming growth factor β type 2, as has been found in mice with abortion due to rejection and/or trophoblast failure. METHODS : Decidual biopsy specimens were taken as near to the placental attachment site as possible under ultrasound guidance from first trimester legal termination (control) patients with recurrent miscarriage and non-viable pregnancy, and from patients with sporadic missed abortion. The tissue was tested for TGFβ-2+ suppressor cells by in situ hybridization, immunohistochemistry, and analysis of supernatants. RESULTS : TGFβ-2-related suppressor molecules similar but not identical to those identified in pregnant mice were released by decidual lymphoid cells. Fifty percent of 14 recurrent miscarriage patients showed a lack of suppressor cells and 59% were subnormal in comparison to 20 controls and 5 sporadic miscarriage patients, where 80–85% of the patients had detectable suppressor cells. CONCLUSIONS : Suppressor cell deficiency is compatible with a role for rejection and/or trophoblast failure in some patients with recurrent miscarriage. Presence of suppressor cells in most patients with missed abortion (4/5) is compatible with an alternative cause of fetal death, similar to findings reported in genetic fetal death mice. 相似文献
994.
995.
Yoshito Matsui Tomoatsu Kimura Noriyuki Tsumaki Haruhiko Nakahara Nobuhito Araki Natsuo Yasui Takahiro Ochi 《Journal of orthopaedic science》1996,1(2):130-135
Recent DNA studies performed by several groups have detected mutations of the gene encoding fibroblast growth factor receptor
3 (FGFR3) in patients with achondroplasia-group disorders, including achondroplasia (ACH), hypochondroplasia (HCH), and thanatophoric
dysplasia (TD). For this study, we analyzed theFGFR3 gene in 31 Japanese patients with typical ACH, four with HCH, three with a condition intermediate between ACH and HCH (ACH/HCH-intermediate),
and one with TD. Of the 31 typical ACH patients, 29 showed a G1138 to A transition and the other two a G1138 to C transversion,
both resulting in a common Gly380Arg substitution in the transmembrane domain of FGFR3. The one TD and the four HCH patients
did not display any mutations in the transmembrane domain of FGFR3. Of the three ACH/HCH-intermediate cases, one patient showed
the Gly380Arg substitution and one did not, and further analysis of the second patient revealed the presence of Asn540Lys
substitution. The first patient was, therefore, genotypically diagnosed as ACH and the second as HCH. Peripheral blood leukocyte
DNA analysis in the remaining ACH/HCH-intermediate patient indicated an unequal ratio of mutant to normal PCR products, possibly
representing a somatic mosaic for the Gly380Arg mutation. Analysis of the common FGFR3 mutation thus appears to help in the
molecular diagnosis of patients with achondroplasia-group disorders. 相似文献
996.
用小鼠热水缩尾法研究了高选择性的CCK-B受体拮抗剂PD134308的镇痛效应。PD134308在小鼠产生的镇痛有剂量依赖关系。阿片受体拮抗剂对抗其镇痛作用,表明阿片受体系统参与介导PD134308的镇痛。PD134308能加强吗啡的镇痛作用,但对α2受体激动剂可乐定的镇痛作用没有影响,表明CCK-B受体拮抗剂对阿片受体系统作用有选择性。脑啡肽酶抑制剂SCH32615加强PD134308的镇痛作用,说明PD134308可能是通过增加内源性阿片物质产生镇痛作用的。另外,PD134308还参与吗啡镇痛耐受性的形成。 相似文献
997.
Inhibition of tissue factor surface expression in human peripheral blood monocytes exposed to cytokines 总被引:1,自引:0,他引:1
Interleukin (IL)-4, IL-10, IL-13 and transforming growth factor beta (TGF-β) are known to regulate several monocyte functions, including inhibition of the synthesis of different cytokines. Using quantitative RT-PCR and flow cytometry analysis we investigated the effects of these cytokines on bacterial lipopolysaccharide (LPS)-induced tissue factor (TF) expression in human monocytes. The effects of IL-4 and IL-10 on monocyte chemoattractant protein-1 (MCP-1)- and C-reactive protein (CRP)-induced TF expression were also studied. A direct comparison revealed that IL-4, IL-10 and IL-13 all down-regulated LPS-induced TF expression in a concentration-dependent manner without the need for priming. In contrast, TGF-β required 4 h of priming to inhibit TF expression induced by LPS. IL-10 was the most powerful inhibitor, causing almost complete inhibition at 5 ng/ml. IL-4 and IL-13 exhibited a significantly lower inhibitory capacity even at concentrations of 100 ng/ml. IL-4 and IL-10 showed similar concentration-dependent inhibition of MCP-1- and CRP-induced TF expression. We also showed that the regulatory effect of the interleukins occurred at the mRNA level. In vivo , these inhibitory cytokines may play an important regulatory role in preventing thrombosis. IL-10, in particular, may be a possible candidate as a TF-preventing drug. 相似文献
998.
999.
Are cytokines possible mediators of cancer cachexia? 总被引:1,自引:0,他引:1
Yoshikazu Noguchi Takaki Yoshikawa Akihiko Matsumoto Gösta Svaninger Johan Gelin 《Surgery today》1996,26(7):467-475
The possible role of cytokines in the development of cancer cachexia was reviewed from the literature. Tumor necrosis factor (TNF)-alpha, interleukin (IL)-1, IL-6, interferon (IFN)-gamma and leukemia inhibitory factor (LIF) can elicit many but not all host changes seen in cancer cachexia, including loss of appetite, loss of body weight, and the induction of acute-phase protein synthesis. However, these cytokines are not always demonstrated in the circulation of the cancer patients. The inability to detect circulating cytokines may be due to their low rate of production, their short half-life and rapid clearance from plasma, or their mode of action (autocrine or paracrine). Different cytokines are induced to stimulate the same response. This is very different from hormonal regulation, where a hormone acts on a cell directly through a specific receptor without depending on other mediators. Specific antibodies including anti-IFN-gamma, anti-TNF and anti-IL-6 antibodies, as well as the cyclooxygenase inhibitor indomethacin, have been used to reverse cancer cachexia. Overlapping physiologic activities make it unlikely that a single substance is the sole cause of cancer cachexia. It is hoped that further investigation on other cytokines and their possible relationships with hormones will help to clarify the mechanisms of cancer cachexia in the near future.This work was supported by a grant from the Japan-Sweden Foundation in 1991. 相似文献
1000.
M. W. J. Boehme R. Waldherr A. Kist S. Riedl R. Walter-Kirst J. Kempeni U. Raeth 《European journal of clinical investigation》1996,26(5):404-410
Cytostatic as well as cytotoxic effects of tumour necrosis factor alpha (TNF-α) therapy have been shown in vitro and in experimental in vivo models. Nevertheless, the mechanism of anti-tumour activity in humans in vivo remains unclear. To determine the role of the vascular lining endothelial cells as important mediators of several immunological interactions, we investigated changes in the levels of the soluble endothelial cell adhesion molecules intercellular adhesion molecule 1, E-selectin and vascular cell adhesion molecule 1 as well as of soluble TNF receptors I and II during systemic therapy with recombinant human rhTNF-α (rhTNF-α). All tests were performed by enzyme-linked immunosorbent assays (ELISAs). The clinical efficacy of the intravenous rhTNF-α therapy was poor. Only one patient with isolated intra-arterial limb perfusion had a delayed, marked, but only temporary necrosis of tumour cells. In contrast, we found a marked, significant and (during therapy) undulating augmented increase in the levels of soluble adhesion molecules as well as of the soluble TNF receptors. Taken together, these data support the hypothesis that a sufficient tumour-specific cellular immunity is required to achieve a clinically apparent efficacy of systemic rhTNF-α therapy in addition to cytokine-dependent inducible activation mechanisms. In this context, the vascular lining endothelial cells might play an important role as mediators of the complex immunological antitumoral activity. 相似文献