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71.
D. W. Behrenbeck A. Dörge H. W. Reinhardt 《Pflügers Archiv : European journal of physiology》1968,300(4):226-243
Zusammenfassung Durch experimentellen Na-Entzug (Mannitolinfusion, Peritonealdialyse) wurde wachen Hunden bis zu 20% des extracellulären Na-Bestandes entfernt.Aus den Inulinverteilungsräumen, der Na-Tagesbilanz vor und nach Salzentzug, sowie den dazugehörigen Plasmanatriumkonzentrationen konnte indirekt eine Verminderung der cellulären Na-Konzentration errechnet werden. Diese Verminderung ergibt sich aus einer Abgabe intracellulären Na und einer Aufnahme von osmotisch frei gewordenem Wasser aus dem ECF-Volumen.Mit dieser Na- und Wasserumkompartimentierung war eine Abnahme der renalen Na-Rejection verbunden.Änderungen des Glomerulumfiltrates und der P
Na-Konzentration bis zu ±20%, sowie die Na-hypotone Expansion des ECF-Volumens haben unter diesen Bedingungen keinen Einfluß auf die Na-Eliminierung. Bei erniedrigter cellulärer Na-Konzentration führte weder der Blockierungsversuch des Aldosterons durch Spironolacton, noch die osmotische Diurese zu einer Zunahme der Na-Rejection.Da bei Übergang von salzarmer zu salzreicher Ernährung mit der Zunahme der ebenfalls indirekt bestimmten cellulären Na-Konzentration [3] immer eine Steigerung der Na-Rejection beobachtet wurde, sehen wir unabhängig von anderen Faktoren in der Änderung der intracellulären Na-Konzentration einen weiteren Parameter für die Anpassung der zum Na-Bilanzausgleich notwendigen renalen Na-Rejection.Vom 1. 1. 1962–1. 3. 1964 Stipendiat der DFG. 相似文献
72.
Bodil Nielsen Gisela SjØgaard Flemming Bonde-Petersen 《European journal of applied physiology》1984,53(1):63-70
Summary During prolonged heavy exercise a gradual upward drift in heart rate (HR) is seen after the first 10 min of exercise. This secondary rise might be caused by a reduction in stroke volume due to reduced filling of the heart, which is dependent upon both hemodynamic pressure and blood volume. Swimming and bicycling differ with respect to hydrostatic pressure and to water loss, due to sweating. Five subjects were studied during 90 min of bicycle exercise, and swimming the leg kick of free style. The horizontal position during swimming resulted in a larger cardiac output and stroke volume. After the initial rise in heart rate the secondary rise followed parallel courses in the two situations. The rises were positively related to the measured increments in plasma catecholamine concentrations, which continued to increase as exercise progresssed. The secondary rise in HR could not be explained by changes in plasma volume or in water balance, nor by changes in plasma [K]. The plasma volume decreased 5–6% (225–250 ml) within the first 5 to 10 min of exercise both in bicycling and swimming, but thereafter remained virtually unchanged. The sweat loss during bicycling was four times greater than during swimming; but during swimming the hydrostatic conditions induced a diuresis, so that the total water loss was only 25% less than during bicycling. 相似文献
73.
Uehara A. Iwamoto T. Shigekawa M. Imanaga I. 《Pflügers Archiv : European journal of physiology》1997,434(3):335-338
A conventional patch-clamp technique was used to record the whole-cell current from the cloned canine cardiac Na+/Ca2+ exchanger NCX1 overexpressed in a fibroblast cell. Ca2+ was extracellularly applied to the Na+-loaded cell to activate the outward current by operating the reverse mode of NCX1. No measurable outward current was ever
elicited from the nontransfected cell. Na+/Ca2+ exchange blocker 5 mM Ni2+ or 3 μM KB-R7943 that was applied extracellularly abolished the outward current. With 140 mM external Li+ (replacing Na+), the outward current was transient during the Ca2+ application. In contrast, with 140 mM external Na+, the outward current was maintained without any inactivation during the Ca2+ application. I–V relations predicted from the whole-cell clamp protocols used were obtained both before and during the Ca2+ application. The exchanger whole-cell currents are thus successfully detectable from NCX1 which is overexpressed in this stable
transfectant system.
Received: 28 February 1997 / Accepted: 9 April 1997 相似文献
74.
G. A. Cherednichenko A. A. Moibenko I. A. Butovich S. A. Ogii 《Bulletin of experimental biology and medicine》1995,120(3):895-897
In thisin vitro study using a purified sarcolemmic fraction of guinea pig myocardium, the 13(S)-hydroperoxide of linoleic acid (13-HPODE)
increased in a dose-dependent manner the permeability of myocardial sarcolemma to Ca ions in concentrations above 10 μmol/liter,
stimulated Na/Ca exchange there in concentrations from 0.1 to 10 μmol/liter, and exerted a digitalis-like action on sarcolemmic
Na,K-ATPase in concentrations between 0.1 and 100 μmol/liter (IC50=20 μmol/liter). The results indicate that the linoleic acid hydroperoxide may be an effective modulator of sarcolemmic Ca2+ transport and of membrane-bound enzymes.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N
o
9, pp. 255–257, September, 1995
Presented by D. F. Chebotarev, Member of the Russian Academy of Medical Sciences 相似文献
75.
Mitchell A. Watsky Kim Cooper James L. Rae 《Pflügers Archiv : European journal of physiology》1991,419(5):454-459
Voltage-gated, tetrodotoxin(TTX)-blockable sodium channels are found in most excitable cells and are the primary contributors to action potentials generated by many of these cells. To date, there has only been one report of a non-cultured vertebrate epithelial cell type containing TTX-blockable Na+ channels: rabbit non-pigmented ciliary body epithelial cells [Cilluffo MC et al. (1991) Invest Opthalmol Vis Sci 32:1619–1629], and three reports of cultured epithelial cells containing TTX-blockable Na+ channels: rabbit non-pigmented and pigmented ciliary body epithelium [Ciluffo MC et al. (1991) Invest Opthalmol Vis Sci 32:1619–1629; Fain GL, Farahbakhsh (1989) J Physiol (Lond) 417:83–103] and human lens epithelium [Cooper K et al. (1990) J Membr Biol 117:285–298]. We report here the presence of sodium currents in two different non-cultured, freshly dissociated transporting epithelial cell types: the rabbit corneal endothelium and the frog lens epithelium. We also report the occurrence of sodium currents in six additional cultured ocular epithelial cell types from three different species. These currents have a current/voltage (I/V) relationship consistent with traditional voltage-gated Na+ currents, are quinidine- and TTX-blockable (of the low-affinity TTX-sensitive type), and disappear following bath substitution of Na+ with Cs+ or K+. 相似文献
76.
Schultheiss G Lán Kocks S Diener M 《Pflügers Archiv : European journal of physiology》2005,449(6):553-563
During inflammatory bowel disease, reactive oxygen metabolites are released by phagocytes reacting with intraluminal NH3 to produce monochloramine (NH2Cl). NH2Cl is assumed to play role in the pathogenesis of inflammation-associated diarrhoea, as it is able to induce intestinal secretion. The aim of the present study was to determine the action sites of NH2Cl in rat colonic epithelium with Ussing chamber and fura-2 experiments. In intact mucosa, NH2Cl (5·10–6–10–4 mol·l–1) evoked a concentration-dependent increase in short-circuit current (Isc), consistent with the induction of anion secretion, as demonstrated by anion substitution and transport blocker experiments. When the apical membrane was permeabilised by the ionophore nystatin, two basolateral action sites of NH2Cl (5·10–5 mol·l–1) could be identified, i.e. an increase in the K+ conductance and a stimulation of the Na+–K+ pump. When tissues were basolaterally depolarised by a high K+ concentration, the stimulation of an apical Cl– conductance by NH2Cl was observed. In isolated colonic crypts loaded with the Ca2+-sensitive fluorescent dye fura-2, NH2Cl (5·10–5 mol·l–1) evoked an increase in the intracellular Ca2+ concentration. This increase was independent from the presence of Ca2+ in the extracellular medium, but was inhibited by blockade of intracellular sarcoplasmatic, endoplasmatic Ca2+-ATPases with cyclopiazonic acid (10–5 mol·l–1). The NH2Cl-evoked Ca2+ release was sensitive against inhibition of ryanodine receptors with ruthenium red (5·10–5 mol·l–1) and against inhibition of inositol-1,4,5-trisphosphate (IP3) receptors with 2-aminoethoxydiphenylborate (10–4 mol·l–1). Both blockers also inhibited the NH2Cl-induced increase in Isc. These results indicate that an intracellular Ca2+ release via ryanodine and/or IP3 receptors is involved in oxidant stimulation of anion secretion in rat colon. 相似文献
77.
The possibility of involvement of a Na–Ca exchange mechanism in the contractile responses induced by a reduction of external Na concentration ([Na]0) has been studied in isolated guinea-pig aorta. Low-Na (11.9 mM) solution (Lisubstituted) produced a contraction in ouabain-treated muscles in the presence of phentolamine (10–6 M). The magnitude of the contraction was dependent on the duration of the pretreatment with ouabain (2×10–5 M). Ca-free solution, but not verapamil (10–6 M), abolished the contraction induced by low-Na solution. The muscles were loaded with various amounts of Na by incubating the tissue with ouabain and varying [Na]0 (11.9–148.7 mM) in the absence of Ca. The magnitude of the contractions induced in these muscles by low-Na solution containing Ca (2.5 mM) was dependent on the cellular Na content. Loss of cellular Na into low-Na solution followed a single exponential time course and the rate coefficient of Na-loss in the presence of external Ca was about twice as great as in the absence of Ca. Cellular45Ca uptake in low-Na solution was significantly greater in Na-loaded tissues (pretreated with ouabain for 3 h) than in normal tissues. The45Ca uptake in low-Na solution was not inhibited by verapamil. These results suggest that the contraction induced by low-Na solution is caused by a Ca influx which is dependent on internal Na (a Na–Ca exchange mechanism). 相似文献
78.
Jacques Chanard Tilman Drüeke Eric Pujade-Lauraine Bernard Lacour Jean-Louis Funck-Brentano D. Coraboeuf 《Pflügers Archiv : European journal of physiology》1976,367(2):169-175
Summary To investigate whether intestinal calcium absorption parallels that of sodium following extracellular fluid volume expansion, the effects of saline loading on intestinal transport of calcium. sodium and water were studied in rats by perfusing jejunal loops in situ.After calcium-free saline infusion net calcium absorption was reversed similar to that of sodium and water and net secretion occurred. Concurrently, blood-to-lumen (b-l) calcium flux, measured using45Ca, increased significantly (P<0.001). Following expansion with calcium-containing Ringer a similar reversal of net calcium, sodium and water flux was also observed. Again, the b-l calcium flux increased but to a significantly lesser extent (P<0.05). Plasma ionized calcium remained unchanged after calcium-rich Ringer loading, but decreased significantly (P<0.001) when calcium was omitted from the solution. Plasma immunoreactive parathyroid hormone was unchanged after expansion with the calcium containing solution but increased following calcium-free infusion.It is concluded that after extracellular fluid volume expansion: 1. net jejunal calcium absorption is decreased; 2. the decrease parallels that of sodium and water; 3. b-l calcium transport is enhanced to a greater degree by calcium-free Ringer infusion than by a calcium-rich solution. This difference could be the result of increased parathyroid hormone secretion. 相似文献
79.
Keld Kjeldsen Aage Nørgaard Torben Clausen 《Pflügers Archiv : European journal of physiology》1985,404(4):365-373
The relationship between the number of3H-ouabain binding sites and the Na, K-pump mediated K-uptake has been characterized in rat soleus muscle. By brief exposure to3H-ouabain (1×10–6–1×10–5mol/l) in vitro, it could be measured that 19–94% of the ouabain binding sites had been occupied. This was associated with a proportionate decrease in the ouabain suppressible K-uptake indicating that under strictly standardized conditions, measurements of3H-ouabain binding sites quantify functional Na,K-pumps.When 3 week old rats were K-depleted for a further week followed by K-repletion 2 h before measurements, the3H-ouabain binding site concentration was 61% lower than in age-matched control soleus muscles. However, the ouabain suppressible K-uptake was only reduced by 35% partly because intracellular Na remained higher in the muscles obtained from K-depleted rats.From the 1st to the 4th week of life, the3H-ouabain binding site concentration increased 2.9-fold. In contrast, the ouabain suppressible K-uptake decreased by a factor 3.5. Accordingly, in muscles from 1 week old rats, the ouabain suppressible K-uptake per3H-ouabain binding site was 10-fold higher than in muscles from 4 week old rats. This difference could not be accounted for by changes in intracellular Na, total or extracellular water. It may be related to differentiation and change in structure.On the basis of the present results and those reported in the literature for mouse and frog skeletal muscle it was calculated that under resting conditions at 30°C in vitro, isolated skeletal muscles only utilize between 3 and 25% of their total capacity for active Na, K-transport. Therefore, variations in the total Na, K-pump capacity may not readily be detected in measurements of the ouabain suppressible rate of K-uptake. 相似文献
80.
Helen E. Miley Darren Holden Richard Grint Leonard Best P. D. Brown 《Pflügers Archiv : European journal of physiology》1997,435(2):227-230
This study investigated regulatory volume increase (RVI) in rat pancreatic β-cells. Volume changes in isolated β-cells were
measured by a video-imaging method. Cell shrinkage was induced by exposure to solutions made hypertonic by the addition of
100 mM mannitol. In HEPES-buffered solutions, β-cells exhibited an RVI which was almost completely abolished by 10 μM bumetanide.
These data indicate that Na+-2Cl–-K+ cotransporters make a major contribution to RVI in β-cells. In HCO3
–-buffered solutions, however, an RVI was observed in the presence of 10 μM bumetanide. This bumetanide-insensitive component
of RVI was inhibited by 100 μM amiloride or 100 μM 4,4’-diisothiocyanatostilbene-2,2’-disulphonic acid (DIDS). These data
suggest that, in addition to the Na+-2Cl–-K+ cotransporter, functionally coupled Na+-H+ exchangers and Cl–-HCO3
– exchangers may also contribute to RVI in pancreatic β-cells.
Received: 3 July 1997 / Received after revision and accepted: 1 September 1997 相似文献