Ligularia fischeri leaf extract prevents the oxidative stress in DBA/1J mice with type II collagen-induced arthritis

The generation of free radicals has been implicated in the causation of several diseases and compounds that can scavenge free radicals have great potential in ameliorating these disease processes. The present study was aimed to investigate the possible antioxidant potential of Ligularia fischeri leaves on collagen type II induced arthritis (CIA) in DBA/1J mice. Ligularia fischeri extracts (100 and 200 mg kg(-1)) were orally administered to DBA/1J mice once daily for 49 days after initial immunization with type II collagen. The levels of oxidant products and the activities of antioxidant enzymes were determined in the serum and organs obtained at day 50. The induction of arthritis significantly increased malondialdehyde (MDA) in the serum and kidney, and oxidized proteins such as protein carbonyl (PCO) and advanced glycation end-products (AGE) in the serum, liver, spleen and kidney. Oral administration of Ligularia fischeri extract at doses of 100 and 200 mg kg(-1) reduced the levels of MDA in the serum and kidney, and PCO and AGE in the serum, liver, and kidney compared with CIA mice. Moreover, treatment with Ligularia fischeri extract dose-dependently increased the activities of superoxide dismutase, glutathione peroxidase and glutathione reductase in the spleen compared with CIA mice. These results suggest that Ligularia fischeri exerts antioxidant actions in the CIA mouse model.     

HPLC-ELSD测定多种千斤拔中的β-谷甾醇

目的 采用HPLC法测定腺毛千斤拔、宽叶千斤拔的根茎及大叶千斤拔、蔓性千斤拔不同产地的根中β-谷甾醇的含量.方法 色谱柱为Kmmasil C_(18)(250 mm×4.6 mm,5 μm),流动相为甲醇,流速1.0 mL·min~(-1),柱温25 ℃.结果 β-谷甾醇0.65～3.25 μg与峰面积呈良好的线性关系,平均回收率为99.40%,RSD=0.87%(n=9).腺毛千斤拔、宽叶千斤拔的根和茎中β-谷甾醇含量均较高;湖北宜昌的蔓性千斤拔及成都华西植物园的大叶千斤拔的根中β-谷甾醇含量最高,其他产地含量较低;大叶千斤拔根中β-谷甾醇的含量较蔓性千斤拔高.结论 所用方法简便、快速、准确,可控制千斤拔药材的质量.     

掌叶橐吾根镇咳作用的初步研究

目的研究掌叶橐吾根的镇咳作用。方法通过小鼠氨水刺激致咳试验对掌叶橐吾根提取物的咳嗽潜伏期及3min内的咳嗽次数进行考察。结果与空白对照组比较,掌叶橐吾根水提物能减少浓氨水诱发小鼠咳嗽次数、延长小鼠咳嗽潜伏期（P〈0．05）;掌叶橐吾根醇提物能显著减少小鼠咳嗽次数、延长小鼠咳嗽潜伏期（P〈0．05）。结论实验结果表明,掌叶橐吾根提取物对浓氨水所致小鼠咳嗽具有较明显的镇咳效果,掌叶橐吾根醇提物的镇咳和延长咳嗽潜伏期的效果比掌叶橐吾根水提物明显。     

Four new compounds from the roots of Uvaria macrophylla

Four new compounds, uvamalols A–C (1–3) and uvarimacrophin A (4), have been isolated from the roots of Uvaria macrophylla. Their structures have been elucidated by spectroscopic methods. The relative configurations of uvamalols A–C have been established by NOE experiments, and the relative stereochemistry of uvarimacrophin A inferred from the diagnostic NMR data by comparison with known model compounds.     

A new norsesquiterpenoid from Ligularia virgaurea

A new eremophilane norsesquiterpenoid (1), together with a known eremophilane sesquiterpenoid (2), was isolated from the leaves of Ligularia virgaurea. The structure of 1 was elucidated by a combination of spectroscopic analysis (IR, 1D NMR, 2D NMR, and HR-ESI-MS), and its absolute configuration was determined by a single-crystal X-ray diffraction experiment (with copper radiation). The known compound 2 was identified by comparison of its physical and spectral data with those reported in the literature. Compound 1 was assayed for its cytotoxic activities against human cervical carcinoma cell (HeLa) and human small cell lung cancer cell (NCI-446) lines.     

裸花紫珠、大叶紫珠和广东紫珠的研究进展

为保护资源,去伪存真,并且为裸花紫珠、大叶紫珠、广东紫珠的深入研究提供参考,本文根据查阅的相关文献资料,从化学成分、药理作用及质量标准等方面,对近5年的研究结果进行概述。     

大叶钩藤的化学成分研究

目的：研究中药大叶钩藤非生物碱部 分的化学成分。方法：柱色谱等方法进行分离,NMR等波谱学方法进 行结构鉴定。结果与结论：分离得到6个化合物,乌索酸、3β-6β-23-trihydroxyurs-12-en-28-oic acid、3β,6β,19α-trihydroxyurs-12- en-28-oic acid、表儿茶素、β-谷甾醇、胡萝卜苷,均为首次从该植物中获得。     

秦艽细胞悬浮培养研究(Ⅰ)

目的建立秦艽细胞悬浮培养体系。方法通过小细胞团法优选细胞系并考察不同培养基、细胞种龄、初始pH值、培养温度、摇床转速对秦艽悬浮细胞生长及龙胆苦苷积累的影响。结果适于秦艽细胞悬浮培养条件为:MS培养基为基本培养基,用15d种龄的细胞接种,初始pH7、25℃,110r/min,摇瓶培养。结论通过摇瓶培养建立了良好的秦艽细胞悬浮培养体系。不同培养基对细胞生长及龙胆苦苷积累的显著影响提示某些无机离子有重要的调控作用。     

中西医结合治疗子宫肌瘤

目的 :比较离舌橐吾( Ligularia veitchiana (Hemsl.) Greenm)的两种苯并呋喃衍生物5, 6-二甲氧基-2-异丙基-苯并呋喃(5,6-dimethoxy-2-isopropenyl-benzofuran)( 1 ),5-乙酰基-6-甲氧基-2-异丙基-苯并呋喃(5-acetyl-6-methoxy-2-isopropenyl-benzofuran)( 2 )对人肝癌细胞HepG2和正常肝细胞L02的毒性,并分析其可能机制。 方法 :用台盼蓝排染法检测化合物细胞毒性、用DCFH-DA荧光分光光度计法检测细胞活性氧含量、用钼酸铵比色法检测过氧化氢酶活性。 结果 :化合物 1和2 对L02的IC₅₀值分别为(171.2 ± 3.3) μg · mL^-1和(79.0 ± 4.1) μg · mL^-1,高于对HepG2的IC₅₀值(84.2±6.5)μg · mL^-1和(65.2 ± 1.9) μg · mL^-1,表明肿瘤细胞比正常细胞对化合物更加敏感。用化合物 1和2 IC₅₀处理细胞48 h,测得HepG2细胞活性氧分别是对照组的1.6倍和3.2倍;L02细胞活性氧分别是对照组的1.2倍和1.8倍。化合物 1和2 处理后,HepG2细胞过氧化氢酶活力分别从对照组的(17.2±1.7)U ·mg^-1Pr.下降到(12.8±0.4)和(6.4±0.1)U ·mg^-1Pr.;L02细胞过氧化氢酶活力分别从对照组的(17.7±1.2) U ·mg^-1Pr.下降到(14.3±1.5) 和(8.6±0.5)U ·mg^-1Pr.;HepG2过氧化氢酶活力降低幅度大于L02。 结论 :二化合物对HepG2的毒性和活性氧的增幅均大于L02,同时HepG2过氧化氢酶活力降幅也大于LO2。据此推测二化合物细胞毒性与活性氧有关。     

云南地区大叶千斤拔不同种质的异黄酮含量比较△

目的:建立大叶千斤拔中异黄酮含量测定方法,对比云南不同地区大叶千斤拔的药材质量,初步明确影响异黄酮含量的因素.方法:以染料木素为对照品,对紫外分光光度法测定大叶千斤拔中异黄酮的含量进行方法学考察,并用此方法测定采自云南不同地区野生及栽培的26份大叶千斤拔中异黄酮的含量.结果:采集野生样品中异黄酮的平均含量为0.990‰,其中屏边县的种质含量最高,为1.553‰,而绿春县(B)的种质含量最低,为0.652‰.分别栽培在3个地点的同一份大叶千斤拔种质,异黄酮含量有明显差异;在同一栽培条件下不同来源的大叶千斤拔种质异黄酮含量也有明显差异.结论:种质和环境因子对大叶千斤拔有效成分的积累均有影响.