不同采收期板蓝根的质量考察

目的:考察不同采收期板蓝根的质量。方法:对1a、2a生板蓝根药材进行性状、显微鉴别,用高效液相色谱法测定药材中腺苷、靛蓝及靛玉红的含量。结果:1a生者韧皮部宽广,薄壁细胞排列疏松;2a生者韧皮部窄,薄壁细胞排列紧密。采挖后未及时晒干或贮藏不善易致其断面变色,靛蓝含量增高,腺苷、靛玉红含量显著降低;生长期过短及2a生板蓝根中的腺苷、靛蓝及靛玉红含量较低。结论:需进行板蓝根规范化种植,并进行质量监控,避免因产地、种植采收时间、初加工及贮藏方法等不同而引起的质量差异过大。     

悦康外感凉茶质量标准研究

目的:建立悦康外感凉茶的质量标准。方法:采用薄层色谱(TLC)法鉴别方中的大青叶、板蓝根、连翘、三丫苦、岗梅;采用高效液相色谱法测定大青叶、板蓝根中靛玉红的含量。结果:TLC的斑点清晰、分离度好。靛玉红的检测浓度在1.52～15.2μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9997);平均加样回收率为101.61%,RSD=1.41%(n=6)。结论:本方法操作简便,结果准确可靠、重现性好,所建标准可用于该制剂的质量控制。     

LC-APCI-MS method for detection and analysis of tryptanthrin, indigo, and indirubin in daqingye and banlangen

A rapid, selective, and sensitive LC-APCI-MS method is developed in this study for detecting and analyzing tryptanthrin, indigo, and indirubin in daqingye and banlangen, which are, respectively, the leaves and roots of Isatis indigotica and Strobilanthes cusia in traditional Chinese medicine. The detection of the three active components is linear in concentrations ranging from 100 to 1500 ng/mL, the squared correlation coefficient is higher than 0.996, the precision as measured by the relative standard deviation is no larger than 9.5%, and the recovery is greater than 86.6%. The analysis of the 21 banlangen samples led to considerably different conclusions on the contents of tryptanthrin, indigo, and indirubin in fresh leaves versus those in dried leaves. These results should shed some light on future plant selection and breeding. Compared with the traditional TLC and HPLC-UV methods, the new LC-APCI-MS approach has proven to be an optimal tool for detecting and analyzing the three marker compounds in the Chinese herbal medicines of daqingye and banlangen.     

大青叶配方颗粒质量标准研究

目的：建立大青叶配方颗粒的质量标准。方法：采用薄层色谱法鉴别配方颗粒中的靛蓝、靛玉红,采用醇溶性浸出物测定法中的热浸法测定浸出物的含量,采用高效液相色谱法测定靛玉红的含量。结果：薄层色谱斑点清晰,分离度好;浸出物的含量限度初步拟定为应不少于12.0%;含量测定中,靛玉红在0.50-8.04μg·ml^-1质量浓度范围内线性关系良好（r=0.999 9）,平均回收率为99.41%,RSD为1.21%（n=9）,并初步确定靛玉红的含量应不得少于50.0μg/袋。结论：建立的定性定量方法操作方便、结果准确、专属性强,可用于大青叶配方颗粒的质量控制。     

RP-HPLC法测定消炎退热颗粒中靛玉红的含量

目的:测定消炎退热颗粒中靛玉红的含量.方法:采用十八烷基硅烷键合硅胶柱;流动相为甲醇-水(75:25);检测波长为 544 nm;流量为 1.0 mL·min-1.结果:靛玉红平均回收率为101.8%,RSD为1.2%(n＝5).结论:本法操作简便,结果准确,可以作为消炎退热颗粒的质量控制方法.     

靛玉红肟及其金属配合物靛玉红肟-Cu(Ⅱ)的合成

为了寻找新的抗白血病药物,我们以靛玉红为原料合成了靛玉红肟及其金属配合物靛玉红肟-Cu(Ⅱ),并用重结晶的方法进行了分离提纯。靛玉红肟的分子结构通过红外光谱、元素分析以及晶体结构分析确定。靛玉红肟-Cu(Ⅱ)的分子结构通过红外光谱、元素分析确定。初步抗白血病活性试验表明,靛玉红肟具有抑制作用,靛玉红肟-Cu(Ⅱ)则无效。     

靛玉红对溃疡性结肠炎小鼠CD4CD25Treg细胞Foxp3表达的影响

目的观察青黛及其有效成分靛玉红对溃疡性结肠炎（UC）小鼠脾脏CD4CD25Treg细胞Foxp3表达的影响,探讨其治疗uc的作用机制和靶点。方法将50只BABL／c小鼠随机分为正常组、模型组、靛玉红组、青黛组和5-ASA组。除正常组外,其余小鼠采用自由饮用5％葡聚糖硫酸钠（DSS）溶液5天,制备DSS诱导的UC小鼠模型。于造模后第5天开始,予各组小鼠给药治疗7天。观察各组小鼠一般状况及结肠组织病理学改变,同时检测脾脏CD4CD25Treg细胞Foxp3的表达变化。结果与正常组比较,模型组小鼠CD4CD25Treg细胞Foxp3的表达显著减少（P〈0．01）;各治疗组CD4CD25Treg细胞Foxp3的表达较模型组升高（P〈0．01）。结论青黛及其有效成分靛玉红可通过上调CD4CD25Treg细胞Foxp3表达以防治UC。     

大鼠全血中靛玉红的含量测定及其药动学研究

目的建立大鼠全血中靛玉红含量测定方法及大鼠体内靛玉红的药动学行为研究。方法采用超声、碱化及液-液萃取等方法处理全血样品;色谱条件:GL ODS C18(4.6×250mm,5μm)色谱柱,流动相为甲醇-5%甲酸(68∶32,v/v),流速:1mL.min-1,检测波长289nm为检测条件;以5.6mg.kg-1的剂量大鼠尾静脉i.v.和200mg.kg-1的剂量大鼠i.g.给药,采用非那雄胺为内标物,测定给药后全血中靛玉红的含量并用DAS 2.1.1软件计算药动学参数。结果靛玉红在5.55~1110ng.mL-1的浓度范围线性关系良好,最低定量限为5.55ng.mL-1,提取回收率71.8±6.4%~80.7±6.5%;大鼠灌胃和静脉注射给药后其体内药动学过程分属二室模型和三室模型,绝对生物利用度10.19%。结论实验表明本法操作简单,灵敏度高,重现性良好,结果准确可靠,可以用于大鼠体内靛玉红的药动学行为研究。     

光合细菌菌剂在提高板蓝根品质中的应用研究

目的：考察在板蓝根种植中应用光合细菌菌剂后板蓝根和大青叶药材品质的提高。方法：采用高效液相色谱法,以甲醇-水（75：25）为流动相,检测波长为289 nm,分析大青叶中靛玉红的含量;按药典要求,用45%乙醇做溶剂测定板蓝根中醇溶性浸出物的含量。结果：使用光合细菌菌剂后,大青叶中靛玉红含量由0.11 mg/g增加为0.26 mg/g,增加了136.4%;板蓝根中浸出物的含量由42.2%增加为47.2%,提高了11.8%。结论：光合细菌菌剂可以提高板蓝根和大青叶的质量。     

Indirubin,a purple 3,2- bisindole,inhibited allergic contact dermatitis via regulating T helper (Th)-mediated immune system in DNCB-induced model

Ethnopharmacological relevance

Indirubin, isolated from Indigo naturalis (Apiaceae) is a purple 3,2- bisindole and a stable isomer of indigo. Although it is known to have anti-inflammatory activities, its mechanism of action has not been elucidated.

Materials and methods

Seven-week-old female BALB/c mice were sensitized with 1-chloro-2,4-dinitrobenzene (DNCB) to induce skin inflammation. Hematoxylin and eosin staining was performed to assess epidermal and dermal hyperplasia, which were determined by measuring the thicknesses of the epidermis and dermis, respectively. We also evaluated serum immunoglobulin E (IgE) levels and cytokines production, such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-4, 6 and Interferon (IFN)-gamma. In addition, we investigated nuclear factor (NF)-κB, IκB-α and mitogen-activated protein (MAP) kinase activities for verifying the molecular mechanism of inflammation.

Results

Indirubin treatment suppressed skin inflammation in DNCB-exposed mice. The skin lesions were significantly thinner in the Indirubin-treated group than in untreated controls, and the hyperkeratosis disappeared. Indirubin reduced the total serum IgE level and cytokines production. In addition, it normalized NF-κB, IκB-α and MAP kinase expression.

Conclusions

Indirubin might be a useful treatment for allergic contact dermatitis via regulating the co-expression of T helper (Th) 1 and 2 cell-mediated immune responses.