一种新的高效液相色谱法选择性测定尿羟脯氨酸

目的　建立一种新的高效液相色谱法,选择性测定尿中羟脯氨酸的含量。方法　采用邻苯二甲醛和芴甲基氯甲酸酯两个衍生剂,先后处理尿标本,以国产 Ｃ８ 柱为固定相,在醋酸钠缓冲液中加入２３ ％ 的乙腈作为洗脱液,３ ,４脱氢脯氨酸作为内标。结果　测定结果的批内、批间变异系数分别为１ ．９２ ％ 和３ ．５８ ％ ,回收率为（９７ ．０ ±３ ．０） ％ ,在３１ ．２５ ～５００ ．００ μｍｏｌ／ Ｌ 范围内线性关系良好（ｒ 为０ ．９９７） 。结论　该法排除了尿液中具有一级胺结构的氨基酸在羟脯氨酸测定中的干扰,特异性好,无需梯度洗脱,适合临床检测需要。     

反相波相色谱法快速测定尿羟脯氨酸

报道了以异硫氰酸苯酯为柱前衍生剂,采用反相液相色谱快速测定尿羟脯氨酸（HYP）的方法。该法的线性范围为31．25～500μmol／L,相关系数0．996,批内、批间的变异系数为2．3％和3．3％,回收率为95．1％,同时测定了68例60～85y老年人尿羟脯氨酸的含量（参考值以尿HYP含量与尿肌酚比值计算）,老年女性的结果（315．4±97．0μmol／g．Cr）显著高于老年男性（198．2±78．7μmol／g．Cr）,P＜0．01。     

The effects of glutamine-enriched feeding on incisional healing in rats

Glutamine (Gln), which is the most abundant free amino acid in the circulation, and also a primary fuel for rapidly dividing cells, was used to determine its effects on incisional healing. The effect of Gln-enriched feeding was investigated using tensile strength measurement, which reflects the quality and speed of regeneration, and by the measurement of tissue hydroxyproline level which correlate directly with the collagen content of wounds. Forty albino Wistar rats in four equal groups received a special diet 7 days prior to surgery and 7 days after surgery. On the 7th day of study a 5-cm dorsal midline skin incision was made and then closed by interrupted sutures. Group 1 received a normal diet for all 14 days; group 2 received a Gln-enriched diet 7 days prior to surgery and a normal diet 7 days after surgery; group 3 received a normal diet prior to surgery and a Gln-enriched diet after surgery; group 4 received a constant Gln-enriched diet. On the 7th postoperative day, tensile strength measurements and hydroxyproline level analyses were done. A preoperative Gln-enriched diet did not show any significant improvement in the tensile strength and hydroxyproline levels on the 7th postoperative day, but a pre- and postoperative, and a postoperative Gln-enriched diet significantly improved the tensile strength and hydroxyproline levels when compared with the normal diet group (P<0.0001). These findings show that wound healing is better when Gln was added in the postoperative, or both pre- and postoperative periods, but not when only added in the preoperative period. Received: 18 May 1998 / Accepted: 16 November 1998     

一种鹿茸复方乳液抗皮肤衰老的实验研究

目的 将鹿茸与5种中药材复配成一种鹿茸复方乳液，并采用皮肤衰老模型小鼠检验该自制乳液的抗皮肤衰老效果。方法 于2020年3月至2021年5月，采用注射D-半乳糖结合紫外光照射诱导小鼠皮肤衰老模型，模型小鼠按体质量均衡随机法分为空白对照组、模型组、阳性组、鹿茸复方低剂量组（5%鹿茸复方乳液）、鹿茸复方中剂量组（10%鹿茸复方乳液）、鹿茸复方高剂量组（15%鹿茸复方乳液组）、鹿茸乳液组（10%鹿茸乳液），每组10只，以同仁堂青春复颜弹力乳液作为阳性组，对照组和模型组不予给药，连续处理42 d，测各组小鼠给药部位皮肤含水率、透明质酸、羟脯氨酸、丙二醛含量、超氧化物歧化酶（SOD）活力。结果 和模型组比较，鹿茸复方低、中、高剂量组均对提高衰老皮肤含水率（50.44±13.77）%比[（67.74±11.37）%、（72.76±8.13）%、（76.00±11.13）%]，羟脯氨酸含量（0.16±0.21）μg/mg比[（0.35±0.12）μg/mg,(0.46±0.18)μg/mg,(0.57±0.23)μg/mg]、SOD活力（48.11±1.83）μ/mg比[（75.94±4.05）μ/...