HPLC法同时测定定喘止咳糖浆中5种成分的含量

目的建立高效液相色谱法同时测定定喘止咳糖浆中苦杏仁苷、和厚朴酚、厚朴酚、橙皮苷和川陈皮素含量的方法。方法采用依利特C_(18)柱,流动相为乙腈(A)-0.2%冰醋酸溶液(B),梯度洗脱;流速:0.8 mL/min;柱温:30℃;检测波长:λ_1=210 nm(苦杏仁苷),λ_2=254 nm(和厚朴酚、厚朴酚),λ_3=283 nm(橙皮苷、川陈皮素)。结果苦杏仁苷、和厚朴酚、厚朴酚、橙皮苷、川陈皮素的线性范围分别为12.10~242.00μg/mL(r=0.999 8)、7.65~153.00μg/mL(r=0.999 3)、6.37~127.40μg/mL(r=0.999 7)、4.29~85.80μg/mL(r=0.999 9)、3.90~78.00μg/mL(r=0.999 2);5种成分的平均加样回收率(n=6)分别为98.62%、96.94%、99.17%、96.85%、97.84%,RSD分别为0.88%、1.12%、1.10%、1.04%、1.55%。结论定喘止咳糖浆中5种成分的含量测定方法准确、可靠。     

百苑止咳颗粒质量标准的建立

摘 要 目的：建立百苑止咳颗粒质量标准。方法: 采用薄层色谱法对桔梗、陈皮、甘草3味药材进行定性鉴别,采用HPLC法测定橙皮苷的含量。使用AlltimaTM C18（150 mm×4.6 mm,5 μm）色谱柱,流动相为甲醇 醋酸 水(35 ∶〖KG-*4〗4 ∶〖KG-*4〗61),流速为1.0 ml·min-1,检测波长为283 nm,柱温为40℃。结果: 薄层鉴别斑点清晰,阴性对照无干扰。橙皮苷进样浓度在5.80～232.08 μg·ml-1范围内与峰面积呈良好线性关系（r＝0.999 7）,平均加样回收率为100.6%,RSD为1.83%（n=6）。结论: 本方法快速、准确、专属性强,可作为百苑止咳颗粒质量控制的方法。     

The effect of active components from citrus fruits on dentin MMPs

ObjectivesThis study was aimed to evaluate the anti-matrix metalloproteinases (MMPs) ability of active components from citrus fruits (hesperetin: Hst, hesperidin: Hsd and naringenin: Nge).MethodsInactivation effects of citrus flavonoids (Hst, Hsd, Nge) at different concentrations on soluble collagenase were measured using a fluorometric assay. Matrix-bound endogenous MMPs activity was evaluated via dry mass loss and hydroxyproline (HYP) release of demineralized human dentin. Demineralized dentin beams were pretreated with 500 μg/mL citrus flavonoids for 10 min. Chlorhexidine (CHX) was used as inhibitor control. Beams pretreated with distilled water served as blank control. Dentin slabs were used for in situ zymography and evaluated under confocal microscopy. Ultrastructure of demineralized collagen fibers was exhibited by Transmission Electron Microscopy (TEM).ResultsCitrus flavonoids exhibited inactivation function on soluble MMPs and the extent of inactivation increased in a dose-dependent manner. The inactivation percent of citrus flavonoids reached above 90% at the concentration of 500 μg/mL. Compared with control group, citrus flavonoids pretreated demineralized dentin beams exhibited less dry mass loss, lower hydroxyproline release and more intact collagen architecture after 15 days storage. Dentin samples pretreated with citrus flavonoids showed lower enzymes activities in in situ zymography.ConclusionsHst, Hsd or Nge have anti-MMPs ability and can preserve dentin collagen from degradation.Clinical Significance: Hst, Hsd and Nge may have the potential to be used in dentin bonding systems and improve the resin-dentin bonding durability.     

Protective effect of Hesperidin and Tiger nut against Acrylamide toxicity in female rats

Phytochemicals that have antioxidant effect play important role in protection against several diseases in humans. This study was carried out to evaluate the efficacy of hesperidin and tiger nut against the early changes that may be related to the toxicity of acrylamide in female rats. 72 Sprague Dawley female rats were divided into six groups (12 rat/group): control group (I); hesperidin (HES) treated group (II); tiger nut (TN) treated group (III); Acrylamide (ACR) treated group (IV); HES-ACR treated group (V); and TN-ACR treated group (VI). There was a significant increase in the levels of serum carcino embryonic antigen (CEA), malondialdehyde (MDA), protein carbonyls (CO), ALT, AST, LDH, urea and creatinine while no significant changes of serum total sialic acid, progesterone (prog) and estradiol (E2) levels, and significant decreases of body weights, catalase (Cat) activity, superoxide dismutase (SOD) activity, reduced glutathione (GSH) level, and glutathione peroxidase (GSH-Px) activity of ACR treated group compared with the control. Our results suggested that supplementation of a diet with hesperidin provided antioxidant defense more significant than tiger nut against the toxicity of ACR in breast, liver and kidney tissues.     

橙皮苷生物活性作用及机制研究进展

陈皮始载于《神农本草经》，为芸香科植物橘及其栽培变种的干燥成熟果皮，别名橘皮。陈皮味苦辛，性温；归肺、脾经，具有“理气健脾，燥湿化痰”的功效。橙皮苷是陈皮黄酮类的主要活性成分之一，其化学结构具有双氢黄酮氧苷结构， 颜色为白色针状晶体，主要存在于芸香科植物橘及其栽培变种的成熟果皮中，其生物活性一直受到人们的广泛关注。近年来随着对橙皮苷的深入研究，其许多药理活性为研究所证实并被广泛应用于医药、食品等科学领域。橙皮苷具有抗炎，抗氧化，抗辐射，抗癌，保护心血管系统，改善血糖、脂质代谢，保护神经系统等生物活性作用。本文结合国内外研究现状，总结前人研究成果，综述了橙皮苷部分生物功效及其活性的机制和最新研究进展，分析和展望其未来发展趋势，以期填补橙皮苷生物活性空白领域，为更好地开发、利用橙皮和橙皮苷，结合其生物活性进行动物实验、临床研究、创新开发药物提供依据和思路。     

HPLC法测定尿石通丸中夏佛塔苷和橙皮苷的含量

摘 要 目的： 建立HPLC法分离和测定尿石通丸中夏佛塔苷和橙皮苷的含量。方法: 以Agilent Zobrax SB C₁₈柱（250 mm×4.6 mm,5 μm）为色谱柱,流动相为乙腈 甲醇 0.4%甲酸溶液,梯度洗脱,检测波长为272 nm,流速为1.0 ml·mim^{-1,进样量:10 μl。结果: 夏佛塔苷在0.000 6 ～0.277 2 mg·ml-1范围内线性关系良好（r=1.000 0）;橙皮苷在0.002 1～0.856 8 mg·ml-1范围内线性关系良好（r=1.000 0）。夏佛塔苷平均回收率为101.83%,RSD=0.27%,橙皮苷平均回收率为98.35%,RSD=0.41%(n=6)。结论: 本方法可用于测定尿石通丸中夏佛塔苷和橙皮苷的含量。}     

HPLC法测定健脾颗粒中橙皮苷的含量

目的:建立以高效液相色谱法测定健脾颗粒中橙皮苷含量的方法。方法:色谱柱为Kromasil C18(200mm×4.6mm,5μm),流动相为乙腈-0.3%磷酸溶液(20∶80),检测波长为283nm,流速为1.0mL·min-1。结果:橙皮苷的检测浓度在9.92～99.75μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为99.1%,RSD=0.2%(n=6)。结论:本方法简便、准确、重现性好,可用于健脾颗粒的质量控制。     

Hesperidin, a flavonoid glycoside with sedative effect, decreases brain pERK1/2 levels in mice

The aim of this work was to evaluate if the intraperitoneal administration of the natural compound hesperidin, in a sedative dose, and neo-hesperidin, a hesperidin structural analog that exerts minor sedative effect, were able to induce changes in intracellular signaling cascades in different areas of the brain.The systemic administration of hesperidin produced a marked reduction in the phosphorylation state of extracellular signal-regulated kinases 1/2 (ERK 1/2), but not of Ca⁺²/calmodulin-dependent protein kinase II α subunit (αCaMKII), in the cerebral cortex, cerebellum and hippocampus. In contrast, neo-hesperidin did not markedly affect the activity of ERK 1/2 in both the cortex and the cerebellum.Taken together, these results demonstrated that intracellular signalling involving a selective decrease in ERK1/2 activation accompanied the depressant action of hesperidin. Even more, the low sedative action of neo-hesperidin correlates with a negligible decrease in phosphorylation state of ERK 1/2 (pERK 1/2), suggesting that low levels of pERK 1/2 in CNS could be a marker of sedative efficacy of flavonoids.     

“抗衰复春片”质量标准的研究

目的：研究抗衰复春片的质量标准。方法：增加TLC法鉴别何首乌、五味子、淫羊藿及RP-HPLC法测定橙皮苷的含量。含量采用Waters C18色谱柱（4.6mm×200mm,5μm）;流动相为甲醇-0.1%磷酸溶液（35∶65）,流速1mL·min-1;检测波长：283nm;用外标法定量。结果：鉴别：阴性对照均无干扰;含量：0.2084～4.1680μg范围内有良好的线性关系（r=0.9999）,平均加样回收率为98.4%（n=6）。结论：所建方法简单、可靠,可用于抗衰复春片的质量控制。     

HPLC法测定五参二连颗粒中橙皮苷的含量

目的：建立HLPC法测定五参二连颗粒中橙皮苷的含量。方法：色谱柱为C18柱（ZORBAXSB,4.6×150mm,5μm）;甲醇-0.5%冰醋酸溶液（38：62）为流动相,流速1.0mL·min-1,检测波长为283nm。结果：橙皮苷的线性范围为0.004144～0.062160mg·mL-1（r=0.9999）,平均回收率为99.96%,RSD为0.48%。结论：该方法快速、准确、重现性好,适用于五参二连颗粒的含量控制。