活血化瘀治则的药理学研究——(三)川芎、人参、丹参配伍对犬心肌组织内氧分压的影响

<正> 继3组活血药对犬急性心肌缺血的影响和对犬冠脉侧支循环影响的实验研究之后,本实验又进行了3组活血药对犬急性心肌缺血时心肌组织内氧分压影响的研究,以便进一步探讨其抗心肌缺血的机制。 一、材料和方法 实验用成年健康杂种犬,分5组。 对照组,川芎组(1g/kg),川芎加人参组(2g/kg),川芎加丹参组(2g/kg),异搏定组(0.4mg/kg),每组动物数3只。     

Ginseng and ginsenoside Rg3, a newly identified active ingredient of ginseng, modulate Ca2+ channel currents in rat sensory neurons

There is increasing evidence that ginseng influences pain modulation. In spite of extensive behavior studies, the detailed mechanism of ginseng actions at the cellular level and the identity of the active substance have not been elucidated yet. Whole-cell patch-clamp recordings were used to examine the modulation of high-voltage-activated Ca2+ channel currents by ginseng total saponins and its various individual ginsenosides in rat dorsal root ganglion neurons. Application of ginseng total saponins suppressed Ca2+ channel currents in a dose-dependent manner. Occlusion experiments using selective blockers revealed that ginseng total saponins could modulate L-, N-, and P-type currents. The co-application of ginseng total saponins and the gamma-opioid receptor agonist, D-Ala(2), N-MePhe(4), Gly(5)-ol-enkephalin (DAMGO), produced non-additive effects in most cells tested and each effect was significantly relieved by a depolarizing prepulse. Overnight treatment of cells with pertussis toxin profoundly reduced the inhibition. Furthermore, we now report that ginsenoside Rg3, among the major fractions of ginseng saponins, is a newly identified active component for the inhibition. These results suggest that the modulation of Ca2+ channels by ginseng total saponins, in particular by ginsenoside Rg3, could be part of the pharmacological basis of ginseng-mediated antinociception.     

软肝汤治疗慢性肝炎后肝纤维化的临床观察

观察软肝汤治疗慢性肝炎后肝纤维化的临床疗效。将60例患者随机分成软肝汤治疗组和人参鳖甲煎丸对照组各30例,观察其对症状,体征,肝功能,肝纤维化指标的改善作用,疗程6个月,结果：治疗组对症状体征有明显改善,其中神疲乏力症状的改善与对照组相比有显著差异P＜0．01,对肝功能指标G,γ－球蛋白的改善治疗前后有显著差异P＜0．05,γ－球蛋白的改善与对照组相比也有显著差异P＜0．01,对肝纤维化指标HA,IVC,PIIIP,LN的改善治疗前后均有显著差异P＜0．01,与对照组相比HA,LN,IVC有显著差异P＜0．05,总有效率治疗组为83．33％, 对照组为73．33％（P＜0．05）,治疗组显效率33．33％,对照组23．33％（P＜0．01）,提示软肝汤对治疗乙型病毒性肝炎肝纤维化,早期肝硬化有较好疗效。     

金翘感冒口服液的质量标准研究

目的研究金翘感冒口服液的质量标准。方法对金翘感冒口服液中主要药物金银花、连翘、甘草进行薄层色谱鉴别;采用RP-HPLC法测定绿原酸的含量。结果薄层色谱检出绿原酸、连翘药材、甘草次酸特征斑点,绿原酸进样量在0.224~1.120μg线性关系良好,平均加样回收率99.81%,RSD为0.89%。结论方法简便易行,灵敏,结果准确,重现性好,可有效控制金翘感冒口服液的质量。     

New staining methodology: Eastern blotting for glycosides in the field of Kampo medicines

Ginsenosides separated by silica gel TLC blotted to a polyvinylidene difluoride (PVDF) membrane treated with a NaIO₄ solution followed by bovine serum albumin (BSA) resulted in a ginsenoside–BSA conjugate on a PVDF membrane. The blotted spot were stained by antiginsenoside Rb1 (GRb1) and Rg1 (GRg1) monoclonal antibodies (MAbs). The newly established immunostaining method, Eastern blotting, was applied for the determination of ginsenosides possessing protopanaxadiol and/or protopanaxatriol in the Kampo medicines. In this method, we developed a new way to separate the ginsenoside molecule into two functional parts using a simple and well-known chemical reaction. The sugar parts were oxidized by sodium periodate to give dialdehydes, which reacted with amino groups of the protein and covalently bound to the adsorbent PVDF membrane. The MAb bound to the aglycone part of the ginsenoside molecule for immunostaining. Double staining of Eastern blotting for ginsenosides using anti-GRb1 and GRg1 MAbs promoted complete identification of ginsenosides in Panax species. This method was validated for immunocytolocalization of ginsenosides in fresh ginseng root. In addition, Eastern blotting for the detection of glycyrrhizin (GC) was also investigated. GC can be clearly determined by Eastern blotting in the Glycyrrhiza species. It is also possible for GC in rat serum to be surveyed by Eastern blotting by simple pretreatment as a further application.     

豫产金银花质量控制的研究

目的：探讨制定金银花质量的方法和指标。方法：用HPLC色谱法对河南不同产地金银花进行绿原酸的含量测定，并对其灰分、酸不溶灰分、砷盐及重金属进行检测。结果：含量测定和检测均获理想结果。结论：找到了较佳方法和指标，为道地药材金银花的质量控制提供参考。     

消炎止痒熏洗剂的薄层层析鉴别

目的：建立消炎止痒熏洗剂的薄层鉴别方法。方法：采用薄层色谱法分析大黄、苦参、黄柏、野菊花，并与消炎止痒熏洗剂相对照。结果：苦参、黄柏在与之对照品色谱相应的位置上显相同颜色的斑点；大黄、野菊花在与之对照药材色谱相应的位置上显相同颜色的斑点。结论：此方法准确，可作为该制剂的质量控制方法。     

舒肤软膏的薄层色谱鉴别

目的:建立舒肤软膏的质量控制标准.方法:采用薄层色谱法对舒肤软膏中的苦参、百部、金银花、青蒿油、薄荷脑进行定性鉴别.结果:在薄层层析色谱中均能检出苦参、百部、金银花、青蒿油、薄荷脑.结论:方法简便、专属性强,可用于该制剂的质量控制.