SRS-82细胞株的无细胞提取液的致白血病活性研究

小鼠SRS-82细胞株的电镜观察表明,其超微结构显示淋巴细胞的特点,在胞质扩大的囊泡内见到池内A型病毒颗粒。SRS-82株的无细胞提取液注射昆明种新生乳鼠24只,其中15只发生白血病,诱发率为62.5%。14只为淋巴细胞白血病,1只为粒细胞白血病。SRS-82株培养上清液的超速离心沉淀物混悬液,注入16只昆明种新生乳鼠,有4只小鼠诱发淋巴细胞白血病。上述研究结果表明。SRS-82株的无细胞提取液和其培养上清液的离心沉淀物皆具有致白血病活性。此外,对病毒与诱发不同的白血病类型之间的关系进行了简要的讨论。     

Prevalence, geographical distribution and genealogical investigations of mutation 188 of lipoprotein lipase gene in the French Canadian population of Québec

Familial lipoprotein lipase deficiency (FLD) is of particular interest to the French Canadian population of Québec since the largest concentration of homozygotes and carriers of this genetic disease in the world resides in this area. We have previously described a missense mutation (M-188) in the lipoprotein lipase (LPL) gene which was present in FLD patients belonging to different ancestries, including a number of French Canadians (Monsalve MV et al. J Clin Invest 1990: 86: 728-734). In the present report, we show that this mutation, although found in largest absolute numbers among French Canadians as compared to other groups in the world, accounts for only a small proportion (24%) of all the LPL mutant alleles in this population. The M-188 occurs either in the homozygote state or as a compound heterozygote with another LPL mutation. Analysis of geographic distribution indicates that the M-188 is more prevalent in western Québec, with the highest carrier rate in the Mauricie region. Genealogical reconstruction leads to the recognition of four founders for M-188, all emigrants from France to Québec in the 17th century.     

培养细胞整装内质网三维结构的多态性

用高锰酸钾－锇酸固定法制备了５种培养细胞整装内质网标本，并在扫描电镜下对其三维结构进行了观察。观察结果表明内质网是由膜性小管构成的贯穿整个细胞质的管囊网络样膜性区室，并以多种形态深入到细胞伪足及突起中；细胞质中内质网则表现为簇状网络（见于ＧＣＭ３Ｔ３细胞）、多态性多孔扁囊样网络、筛网状网络、条索状网络、大孔条索网状和细孔扁囊样分区网络、不规则管网状和多孔管囊分区网络（见于ＣＶ－１细胞）、细管网络（见于ＣＣＬ１８７和ＣＣＬ２２９细胞）、球囊网络（见于ＣＣＬ１８７和Ａ４３１细胞）和不规则管网状网络（见于Ａ４３１细胞）等。内质网的这种多态性提示它是一种高度可变的结构，其可变性可能与细胞特性、分化程度、细胞功能状态及细胞骨架系统的分布变化等因素有关。     

肾盂加肾后唇实质段间线切开治疗巨大鹿角形肾结石(附24例报告)

目的：探讨。肾内型。肾盂并发巨大鹿角型。肾结石的手术疗效。方法：采用。肾盂加。肾后唇实质段间线切开取石术治疗24例25侧巨大鹿角型。肾结石。结果：本组手术顺利取尽结石者22例23侧。肾，术后。肾内残留小结石（直径〈5mm）2例，经ESWL治愈。随访3个月，25侧术。肾功能良好。结论：该术式不阻断。肾蒂，操作简单，出血少，取石干净，对肾功能影响小，是治疗巨大鹿角型。肾结石较理想的手术方法之一。     

pGenesil-siHBV X 对 HepG2.2.15 细胞 HBV 表达和复制的抑制效果研究

 目的 研究针对HBV X基因区设计的小干扰RNA（siRNA）表达载体质粒pGenesil-siHBV X 对HepG2.2.15细胞HBV表达和复制的抑制效果及特异性。方法 针对HBV X区设计siRNA表达载体质粒pGenesil- siHBV X。分别用培养液（空白对照）、脂质体Metafectene、pGenesil 空载体、pGenesil-siHK（阴性对照）、pGenesil-siAFP（特异性对照）、pGenesil-siHBV X处理或转染HepG2.2.15细胞各3次。于每次转染后24 h，取各组细胞培养上清液，用时间分辨荧光免疫测定技术检测上清液中HBsAg和HBeAg含量，用化学发光法检测AFP含量，用PCR荧光定量技术检测HBV-DNA复制水平。 结果 pGenesil-siHBV X转染能抑制HepG2.2.15细胞对HBV标志物的表达，且抑制作用随转染次数增加而增强。第3次转染后，pGenesil-siHBV X组细胞上清液中 HBsAg、 HBeAg和HBV-DNA检测结果分别为（6.26 ± 1.07）ng/ml 、（0.13 ± 0.05）Ncu/ml和（3.01 ± 0.40）×107拷贝/ml，与空白对照组的（22.50 ± 1.39）ng/ml、（1.12 ± 0.11）Ncu/ml和（12.33 ± 1.28）×107拷贝/ml比较，差异有统计学意义（t值分别为12.80、12.21、9.71，P < 0.05）；pGenesil-siHBV X 转染不影响细胞对AFP的表达（t = 0.18，P = 0.86）。结论 pGenesil-siHBV X可以有效和特异地抑制HepG 2.2. 15细胞HBV-DNA的复制及HBsAg和HBeAg表达。     

A new liver metastatic and peritoneal dissemination model established from the same human pancreatic cancer cell line: analysis using cDNA macroarray

To elucidate the mechanisms of metastasis, we established two sublines HPC-1H5 with a highly liver metastatic cell line and HPC-1P5a with a highly peritoneal disseminating cell line, which were sequentially selected from the parental pancreatic cancer cell line HPC-1. Using these three cell lines, we investigated several biological properties and mRNA levels of differentially-expressed genes involved in cancer metastasis by cDNA macroarray. Microscopic findings for the three cell lines were the same. The tumorigenicity, in vitro growth ability, motile activity, adhesive activity and the production of IL-8 of metastatic sublines were higher than those of parental HPC-1 cells. Particularly, HPC-1H5 cells showed clearly higher levels of IL-8 expression and tumors of HPC-1H5 cells grew faster and bigger than those of HPC-1P5a cells. In cDNA macroarray analysis of HPC-1H5 cells, 22 genes were up-regulated and 44 genes were down-regulated compared with parental HPC-1 cells. In HPC-1P5a cells, 9 genes were up-regulated and 28 genes were down-regulated compared with parental HPC-1 cells. This study provides a demonstration of global gene expression analysis of pancreatic cancer cells with liver metastasis and peritoneal dissemination. Furthermore, our results provide a new insight into the study of liver metastasis and peritoneal dissemination of human pancreatic cancer. This revised version was published online in July 2006 with corrections to the Cover Date.     

High prevalence of mixed genotype infections in hepatitis B virus infected intravenous drug users

The clinical relevance of hepatitis B virus (HBV) genotypes has been documented; however, the prevalence of mixed HBV genotype infections in at-risk groups remains controversial. The HBV genotypes were determined in 325 HBV-infected intravenous drug users (IVDU) who were at a greater risk of multiple exposures to different HBV genotypes by using a newly developed line probe assay. The distribution of HBV genotype was as follows: genotype A alone in 2 (0.6%); genotype B alone in 256 (78.8%); genotype C alone in 10 (3.1%); mixed genotype A and B in 18 (5.5%); genotype B and C in 30 (9.2%); genotype B and D in 1 (0.3%); genotype A and C in 1 (0.3%); and mixed infections of genotype A, B, and C in 3 (0.9%). Clonal analysis confirmed further the existence of mixed genotype infection and recombination between different genotypes. Compared with our previous data, the line probe assay seemed more sensitive than polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) assay in identifying HBV genotype (98.8% vs. 65.0%) and detecting mixed genotype infections (16.3% vs. 0%). In conclusion, the prevalence of mixed HBV infections is substantially higher in IVDU in endemic areas, and the line probe assay is a useful method for rapid genotyping of HBV, with particular reference to the detection of mixed genotype infections.     

The effect of phenytoin in vitro on normal human mononuclear cells and on human lymphoblastoid B cell lines of different Ig isotype specificities

The anticonvulsant drug phenytoin, in less than cytotoxic concentrations, caused significant reductions in Ig secretion by unstimulated or EBV-stimulated normal MNC, as measured by PFC or secretion of Ig into the culture medium. Isotype-specific LBL varied in their sensitivity, the secretion of IgA (1 line) and IgG (3 lines) being reduced by phenytoin near therapeutic concentrations, whereas that of IgM (1 line) was resistant. Six-day exposure of MNC to phenytoin caused no selective depletion of or enrichment for B cells, monocytes or T cell subsets. The results suggest that the reduction in serum Ig levels reported in phenytoin-treated epileptic patients is, at least in part, due to a direct effect of the drug on the B lymphocyte. However, among EBV-activated normal MNC, those secreting IgA were no more sensitive to the drug than those secreting IgG or IgM, and other factors may, therefore, operate to cause the preferential reduction in serum IgA in phenytoin-treated patients.     

骶骨耳状面倾斜度及相关径线

对36具成人骶骨耳状面倾斜度及相关径线测量结果,显示男性耳状面长轴的前倾度显著大于女性;耳状面上部的侧倾度和后倾度显著大于下部;耳状面中间部的宽度及后倾度在男、女性间有显著性差异。     

Biological properties and gene expression associated with metastatic potential of human osteosarcoma

Lung metastasis has a great influence on the prognosis of patients with osteosarcoma. We previously established two high-metastatic sublines, M112 and M132, from the HuO9 human osteosarcoma cell line by in vivo selection. In this study, we newly isolated a high-metastatic subline, H3, and three low-metastatic sublines, L6, L12 and L13, from HuO9 by the dilution plating method. Three high-metastatic sublines produced more than 200 metastatic nodules in the lung, while three low-metastatic sublines produced no or few nodules after injection of 2 × 10⁶ cells into the tail vein of nude mice. There were significant differences in the motility and invasiveness between high- and low-metastatic sublines, whereas the growth rates in vitro and the tumorigenicity in vivo showed no correlation with their metastatic abilities. Early adherence to culture plates was significantly lower in two of three low-metastatic sublines, which occupied smaller surface areas on the culture plates than other sublines did. Comparison of the expression of 637 cancer-related genes by cDNA microarray revealed that seven genes were differentially expressed between high- and low-metastatic sublines. Among them, five genes (AXL, TGFA, COLL7A1, WNT5A, and MKK6) were associated with adherence, motility, and/or invasiveness. These results suggest that the differences in motility/invasiveness and adhesive abilities are key determinants of lung metastasis in osteosarcoma. This revised version was published online in July 2006 with corrections to the Cover Date.