黄药子醇提物不同处理方式对肝毒性的影响

目的:探讨不同极性黄药子醇提物组分导致肝毒性的强弱.方法:将黄药子75%乙醇提取物依次用氯仿、乙酸乙酯、正丁醇萃取,采用SD雄性大鼠进行体内筛选,观察不同部位肝毒性的大小,以血清酶学进行考查.结果:氯仿部位肝毒性作用最强,乙酸乙酯部位次之.结论:黄药子中弱极性化合物组分具有显著的肝毒性.     

胸椎黄韧带骨化症的CT分型及手术方式选择

目的:探讨胸椎黄韧带骨化症的CT分型及手术治疗方法.方法:1997年1月至2006年12月手术治疗胸椎黄韧带骨化症患者48例102个节段,根据CT表现分为3型,单侧型18个节段,双侧型45个节段,两侧融合型39个节段.单侧型将椎板、关节突内侧和未骨化处磨薄,再把骨化物对侧和头尾侧充分减压使其孤立,用枪状咬钳将关节突内侧磨薄处咬开使其游离,齿镊夹住骨块轻提起由中间向外侧剥离摘除骨块;双侧型将椎板、关节突内侧和未骨化处磨薄,先将骨化物头尾侧充分减压,将中间未骨化黄韧带咬除分隔,使两侧骨化物孤立,再按单侧型手术方法逐块处理;两侧融合型将椎板、关节突内侧和未骨化处磨薄,先将骨化物头尾侧充分减压,从对侧关节突内侧磨薄处咬开使骨化物孤立,再将术侧关节突内侧磨薄处咬开使骨化物游离,齿镊夹住骨块轻提起由对侧向术者侧剥离摘除骨块.术前术后采用改良JOA下肢运动功能评分评价运动功能.结果:全部患者顺利完成手术.手术时间平均2.8h,出血量平均290ml.术后无症状加重病例,1例出现脑脊液漏,经保守治疗后痊愈.40例患者随访5～62个月,平均28个月,JOA下肢运动功能评分术前1.8±1.1分,末次随访时为3.7±0.6分,与术前比较差异有显著性(P<0.01).疗效按JOA评分改善率优32例,良6例,可2例,优良率为95%.结论:对胸椎黄韧带骨化症患者根据CT分型采取不同的手术方式可取得满意的治疗效果.     

白花蛇舌草治疗皮肤瘙痒症

白花蛇舌草味甘、淡，性微寒，具有清热解毒，消痈散结，利尿除湿之功效。笔者在临证中，重用白花蛇舌草治疗顽固性皮肤瘙痒症取得满意疗效。举例介绍如下。     

自拟黄棱术草酊外用预防化疗性静脉炎

目的:探讨黄棱术草酊预防化疗性静脉炎的临床效果。方法:将进行化疗的恶性肿瘤患者100例作为临床观察对象,采用黄棱术草酊局部涂布与外敷进行预防,对用药后化疗性静脉炎的发生情况进行观察。结果:总有效率达98%。结论:黄棱术草酊功能全面、药效迅速、效果可靠,是预防化疗性静脉炎安全、迅速、高效的中草药外用制剂。     

Effect of curcumin on nitric oxide synthase expression in Iipopolysaccharide-activated microglia cells and the anti-oxidative effect of curcumin

BACKGROUND: It has been demonstrated that curcumin can increase the activities of various anti-oxidase in blood and tissue, effectively eliminate various free radicals, reduce the production of peroxisome, and alleviate oxidative stress reaction. Whether it has the same effect on microglia? OBJECTIVE: To observe the effects of curcumin on the expressions of inducible nitric oxide synthase (iNOS), nuclear factor-κB (NF-κB), and superoxide dismutase (SOD) in microglial cell line BV stimulated by lipopolysaccharide (LPS). DESIGN: An observational comparative study. SETTING: Research Room of Biochemistry, Medical College of Nantong University. MATERIALS: Mice microglia cell line BV, iNOS and NF-κB reporter gene plasmids were presented by Dr. Bhat.NR. from the Medical University of South Carolina (USA). Curcumin was produced by the Xi'an Branch of China Chengdu Scholar Bio-Tech. Co.,Ltd.; LPS (E.Coli O26:B6), anti-mice iNOS monoclonal antibody, horseradish peroxidase labeled goat-anti-mice IgG were the products of Sigma Company (USA). METHODS: The experiments were carried out in the Research Room of Biochemistry, Medical College of Nantong University from May 2006 to April 2007. ① Detection of iNOS: The cells were seeded onto 24-well plate at the density of 1×105, After the cells had adhered to the cover glasses, the cells were grouped as negative control group (the primary antibody was replaced by phosphate buffered solution PBS); normal control group (the cells were normally cultured); LPS-treated group (the cells were treated with LPS for 24 hours); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours). The expressions of iNOS protein were detected with immunocytochemical staining. ② Determination of iNOS and NF-κB gene activities: According to the introduction of the kit for transfection, iNOS or NF-κB report gene plasmids were transiently transfected with LipofectamineTM2000 liposomes into the cells in the 24-well plate for 24 hours. The cells were divided into normal control group (the cells were normally cultured after transfected with report gene plasmids); blank plasmid group (the cells were normally cultured after transfected with blank plasmids); LPS-treated group (the cells were treated with LPS for 4 hours after transfected with report gene plasmids); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours after transfected with report gene plasmids). The content of luciferase in the cell lysis buffer was determined after cell lysis. ③ Determination of SOD activity: The cells were seeded into culture bottle at the density of 1×106, and the divided into four groups, including normal control group (the cells were normally cultured); LPS-treated group (the cells were treated with LPS for 24 hours); curcumin+LPS group (the cells were treated with curcumin for 1 hour and LPS for 24 hours); vitamin C+LPS group (the cells were treated with vitamin C for 1 hour and LPS for 24 hours). The SOD activity was determined with xanthine oxidase and quantitative colorimetric assay. MAIN OUTCOME MEASURES: The expressions of iNOS protein, iNOS and NF-κB, and the activity of SOD were observed. RESULTS: ① Expression of iNOS protein in microglia: The expression of iNOS protein in the LPS-treated group was obviously higher than that in the negative control group (P < 0.01); Those in the curcumin+LPS group were significantly decreased as compared with that in the LPS-treated group (P < 0.01). ② Expressions of iNOS and NF-κB genes: The expressions of iNOS and NF-κB genes in the LPS-treated group were significantly higher than those in the normal control group (P < 0.01); Those in the curcumin+LPS group were significantly lower than those in the LPS-treated group (P < 0.01). ③ SOD activity: The activity of SOD in the LPS-treated group was significantly lower than those in the normal control group (P < 0.01). It in the curcumin+LPS group and vitamin C +LPS group was significantly higher than that in the LPS-treated group (P < 0.01). CONCLUSION: Curcumin could inhibit the expression of iNOS in the activated microglia, and it also has the abilities in eliminating free radicals and antagonizing lipid peroxidation.     

民族中草药医疗市场监管初探

目的为促进民族中草医的健康发展,保障人民群众就医安全。方法采用现场调研和监督检查相结合的方式对我县县城双江镇的民族民间中草药医疗市场进行查实。结果合法执业者比例很少,大部分为无证非法经营,医疗安全隐患显著存在。结论政府支持,监管到位,群众配合才能切实有效的促进我国民间民族医疗市场的健康发展。     

慢性乙型肝炎中医药治疗中的有关问题

经长期临床观察研究,对慢性乙型肝炎(CHB)的中医药治疗论述了以下有关问题CHB自然病程和中西医治疗策略;介绍治疗CHB中药基本方“二黄双虎汤”;CHB的降酶治疗;CHB“正复胜邪现象”;对HBV携带者的用药经验。     

肝炎颗粒治疗急性病毒性肝炎91例临床总结

诊断标准按1995年北京传染病与寄生虫病学会修订《病毒性肝炎防治方案 (试行)》,共177例住院病人,乙型肝炎123例,甲型肝炎18例,戊型肝炎36例。分为治疗组91例,男69例,女22例,年龄 (35.5±10.4)岁 ;对照组86例,男70例,女16例,年龄 (34.6±12.3)岁。各组在性别、年龄、病史、病程及肝功能异常等方面无明显差异,具有可比性。     

HPLC法测定赶黄草中没食子酸的含量

目的：建立HPLC测定赶黄草中没食子酸含量的方法。方法：色谱柱：DikmaKromasil C18柱（250mm×4．6mm，5μm），流动相：甲醇-0．1％磷酸0．1％三乙胺水溶液（10：90），检测波长：271nm，柱温：30℃，流速：0．8ml／min。结果：没食子酸在0．07～0．56μg范围内具有良好的线性关系（r=0．9999），平均回收率为95．23％，RSD为1．08％。结论：该方法简便、准确、可靠，重复性好，可用于赶黄草的质量控制。