镉离子和维生素C对果蝇寿命及抗氧化系统的影响

目的 研究镉离子(Cd~(2+))和维生素C(Vc) 对野生型果蝇寿命、生育力以及抗氧化系统的影响.方法 将果蝇随机分为5组,分别饲喂含不同浓度的Cd~(2+)的培养基,并加Vc处理,计算果蝇平均寿命、子代数量,测定超氧化物歧化酶 (SOD) 活性和丙二醛 (MDA)含量.结果 随着Cd~(2+)浓度的增加,雌雄果蝇的平均寿命逐渐缩短,子代减少,SOD活性下降和MDA含量上升,而Vc处理组显示对Cd~(2+)的毒害存在显著拮抗效应(P＜0.05或P＜0.01).结论 高Cd~(2+)浓度对果蝇有比较大的损伤,Vc可以缓解毒害,延长果蝇寿命.     

分光光度法测定鱿鱼生殖腺提取物中总磷脂

目的建立鱿鱼生殖腺提取物中总磷脂的测定方法。方法采用乙醇提取、正己烷萃取法得到鱿鱼生殖腺提取物,浓硫酸-高氯酸法消化样品,抗坏血酸-钼蓝法测定无机磷的量,再乘以系数26.31折算成鱿鱼生殖腺提取物中总磷脂的量。结果磷在0.2～1.0μg/mL线性关系良好(r=0.9999),平均回收率为95.06%,RSD为1.09%。结论该方法方便、快速、准确度较高,可用于鱿鱼生殖腺提取物中总磷脂的测定。     

条件培养基抑制L-抗坏血酸钠介导的小鼠黑色素瘤细胞凋亡

目的探讨肿瘤细胞B16F10通过自分泌对抗由L-抗坏血酸钠(VitCNa)介导的凋亡作用。方法常规在75 cm~2培养瓶中培养小鼠黑色素瘤细胞B16F10,待细胞长至90%～95%融合时吸取原DMEM/高糖培养液,用PBS反复清洗后加入无血清的DMEM/高糖10ml在培养箱中培养6～8 h,收集培养液用2500 r/min离心5min后上清用0.22μm滤器过滤,-20℃保存备用,在6孔板中每孔接种1×10~6 B16F10,常规培养24h后,实验组每孔加入2ml条件培养液(含有10%胎牛血清),对照组用普通培养液,两组均用10 mmol/L VitC Na促凋亡,分别在3、6、24 h观察细胞凋亡情况,hoechst染核,收集细胞免疫荧光检测caspase3和TUNEL、RT-PCR检测Bcl2表达。进而分离出条件培养液中相对分子质量大于5 000和小于5 000成分,分别煮沸,用蛋白酶K灭活后对B16F10促凋亡。结果发现条件培养基能够抑制VitC Na介导的小鼠黑色素瘤细胞B16F10凋亡,抗凋亡成分小于5 000,且不能被煮沸和蛋白酶K灭活,提示其是小分子抗凋亡物质。结论 B16F10可以分泌出拮抗细胞凋亡的小分子物质。     

The protective effect of ascorbic acid and thiamine supplementation against damage caused by lead in the testes of mice

Lead is a ubiquitous environmental and industrial pollutant that may have toxic effects on the male. Vitamins may protect against toxic effects of lead in the liver and reproductive system, which is confirmed by our initial research. The aim of this study was to further investigate the protective effects of vitamins （ascorbic acid combined with thiamine） on lead acetate （Pb）-induced reproductive toxicities in mice and study the possible mechanisms underlying these effects. Forty-five male mice were randomly divided into 3 groups, 15 mice in each and received daily intragastric administration with control, Pb （20 mg/kg）, and Pb＋vitamins （ascorbic acid of 420 mg/kg＋thiamine of 30 mg/kg） for 6 weeks, respectively. The Pb-treated animals showed significant decreases in the epididymal sperm count and motility compared to the control group, while the Pb＋vitamins group had significant increases for these variables. Moreover, an increasing apoptosis of germinal cells induced by Pb was reduced by vitamin treatment. Pb induced the activation of Caspase-3, Fas/Fas-L and Bcl-2 with elevated levels, and the adaptor protein primarily regulated signaling through Fas and required for Fas-induced apoptosis. In conclusion, ascorbic acid combined with thiamine exhibited protective effect on reproductive system by inhibiting Pb-induced excessive cell apoptosis.     

迷迭香水溶性成分研究

迷迭香 Rosmarinus officinalis L .系唇形科( Labiatae)迷迭香属植物 ,为常绿灌木。具有抗氧化、抗病毒、抗肿瘤等多方面的功效 ,广泛应用于药品、化妆品和食品等方面。主要成分为二萜酚类、黄酮类、三萜类及精油等 ,其中二萜酚类成分具有明显的抗氧化、抗肿瘤及抗微生物等活性。水溶性成分迷迭香酸在抗炎、抗血栓等方面有明显作用 ,已用于临床。鉴于其多方面的活性 ,该植物已成为全球普遍重视的资源植物之一。为了进一步阐明国产迷迭香的化学成分 ,促进我国对迷迭香的综合利用 ,我们对其水溶性成分进行了较系统的研究。从其茎叶的 95 %…     

斑蝥素贴剂结合维生素C静脉注射辅助治疗带状疱疹1例（英文）

The diagnosis and therapy for herpes zoster(HZ)are frequently associated with consultation of a general practitioner and with hospital care.Although there are several serious complications of zoster such as ophthalmic,splanchnic,cerebral,and motor conditions,the most common and feared in immunocompetent adults is postherpetic neuralgia(PHN).Its definition is controversial.Recent data support the distinction between acute herpetic neuralgia(within 30 d of rash onset),subacute herpetic neuralgia(30 to 120 d after rash onset),and postherpetic neuralgia(defined as pain lasting at least 120 d from rash onset)[1-3].PHN is classified as a neuropathic pain that is associated with mechanical allodynia where normally innocuous tactile stimuli are perceived as painful[2].In actual studies it is reported that the incidence of HZ is 3.2 to 4.1 in 1 000 person-years[4,5].     

络得舒方的抗炎机理探讨

本文探讨了络得舒方的抗炎机理。实验结果,络得舒方对摘除肾上腺大鼠甲醛性足肿胀仍有明显抑制作用(P<0.05～0.01);对正常大鼠肾上腺内VitC含量无明显影响(P>0.05);对热诱导所致红细胞溶解有明显抑制作用(P<0.01);对妊娠大鼠离体子宫的自发性收缩有明显抑制作用(P<0.01);对二磷酸腺苷所致的血小板聚集有明显抑制作用(体内体外用药P值均<0.01)。结果提示:络得舒方的抗炎作用不是主要依赖垂体——肾上腺皮质系统,可能与保护红细胞膜、抑制前列腺素合成酶和/或拮抗前列腺素、抑制血小板聚集有关。     

抗坏血酸钛果蝇致畸效应的观察

近年来,我国将抗坏血酸钛(Ti Vc)用于农牧养殖业,已取得明显的增产效益。本文以果蝇作为致畸快速筛选系统对Ti Vc的致畸效应进行初步研究。     

冬储蔬菜中抗坏血酸含量分析

冬储蔬菜中抗坏血酸含量分析李晔（哈尔滨铁路中心卫生防疫站，哈尔滨１５０００１）中国东北地区冬季漫长，速冻已成为本地区冬季储存蔬菜的一种新型方式。本文采用高效液相色谱分析方法，对鲜菜、速冻菜及熟菜中抗坏血酸的含量进行了分析比较。溶液配制：抗坏血酸标准溶...