Expression of Presenilin-2 and Glutathione S Transferase π and Their Clinical Significance in Breast Infiltrating Ductal Carcinoma

Objective: To investigate the expressions of presenilin-2 (PS2) and glutathione S transferase π (GSTπ) and their roles in prognosis and therapy of breast infiltrating ductal carcinoma. Methods:The paraffin-embedded specimens of 210 patients with breast infiltrating ductal carcinoma were examined by using LSAB immunohistochemistry for the expression of PS2 and GSTπ. Results: The expression rate of PS2 and GSTπ was 49.5% (104/210) and 48.1% (101/210) respectively. The 5-year and 10-year postoperative survival rates in 4 groups, from high to low, were group 1 (PS2 positive expression/GSTπ negative expression), group 2 (PS2 positive expression/GSTπ positive expression), group 3 (PS2 negative expression/GSTπ negative expression) and group 4 (PS2 negative expression/GSTπ positive expression) in turn. Conclusion: The prognosis of the group 1 was the best, followed by the group 2, group 3 and group 4 in turn. These results suggested that the reasonable use of endocrinotherapy and chemotherapy for patients with breast infiltrating ductal carcinoma is necessary.     

Anti-tumor effect of pEgr-interferon-γ-endostatin gene-radiotherapy in mice bearing Lewis lung carcinoma and its mechanism

Background Gene-radiotherapy, the combination of gene therapy and radiation therapy, is a new paradigm for cancer treatment. To enhance anti-tumor effect of gene-radiotherapy, in this study we construct a radiationinducible dual-gene co-expression vector pEgr-interferon(IFN)-γ/- endostatin and studied the anti-tumor effect of pEgr-IFN-γ/-endostatin gene-radiotherapy in mice bearing Lewis lung carcinoma and its mechanism.Methods Gene recombinant technique was used to construct dual-gene co-expression plasmid pEgr-IFN-γ endostatin, and single-gene expression plasmid pEgr-IFN-γ and pEgr-endostatin. The plasmids packed by liposome were injected locally into the tumors of the mice, and the tumors were irradiated with 5 Gy X-ray 36 hours later. The tumor growth rate at different time and mean survival period of the mice were observed.Cytotoxic activity of splenic cytotoxic T-lymphocyte ( CTL), natural killer (NK) cell and tumor necrosis factor (TNF)-α secretion activity of peritoneal macrophages of the mice in various groups were evaluated 15 days after irradiation. The intratumor micro-vessel density was evaluated by immunohistochemical staining 10 days after irradiation.Results The tumorgrowthrate of the mice in dual-gene-radiotherapy group was significantly lower than those in control group, 5 Gy group and single-gene-radiotherapy group at different time after gene-radiotherapy, and the mean survival period of which was longer. Cytotoxic activity of splenic CTL, NK and TNF-α secretion activity of peritoneal macrophages of the mice in dual-gene-radiotherapy group were significantly higher than those in control group, 5 Gy X-ray irradiation group and pEgr-endostatin gene-radiotherapy group 15 days after irradiation. The intratumor micro-vessel density of the mice in dual-gene-radiotherapy group was significantly lower than those in control group, 5 Gy X-ray irradiation group and pEgr-IFN-γ/gene-radiotherapy group.Conclusion The anti-tumor effect of dual-gene-radiotherapy was significantly better than that of single-gene-radiotherapy by combining the enhancement of anti-tumor immunologic function induced by IFN-γ/with the antiangiogenesis function of endostatin.     

血管活性多肽与心血管及内分泌疾病

心血管生物活性物质是维持人体生命活动最重要的物质基础,它们在调节和整合心血管系统的生长发育及疾病的发生发展等方面均起到了重要的作用.近年来,心血管生物活性肽对内分泌代谢功能的调节作用及在内分泌和代谢性疾病发生机制及诊断、治疗和早期干预措施中的作用正被大家重视,其基础和临床研究不断取得新的进展,已成为当前生命科学研究中最活跃、发展最迅速的领域之一.     

第八届全国内分泌遗传代谢病学术会议纪要

第八届全国儿科内分泌遗传代谢病学术会议于2004年10月11日至15日在成都举行。来自全国26个省、市和地区的214名代表出席了会议;大会共收到论文207篇,内容涉及性腺、糖尿病、甲状腺、垂体、肥胖和遗传代谢等疾病。会议邀请11位中、外专家进行了专题讲座,内容为：(1)从青春发育年龄提前的年代趋势解析至对女性性早熟的再认识;(2)遗传代谢性疾病的治疗;     

心力衰竭生物学治疗的现状和展望

1987年应用血管紧张素转换酶抑制剂（ACEI）治疗心力衰竭（心衰）的临床试验CONSENSUS,不同于以往应用正性肌力药和血管扩张剂治疗心衰的试验,使心衰的死亡率成功地降低了27％,奠定了神经内分泌抑制剂治疗心衰的基础。20世纪90年代中、后期,3个大型、经典的β受体阻滞剂治疗心衰的试验CIBISⅡ、MERIT-HF、COPERNICUS,进一步使心衰死亡率降低34％-35％。     

21羟化酶缺乏症患者最终身高影响因素探讨

先天性肾上腺皮质增生症(CAH)是一组由于肾上腺皮质激素合成酶缺乏导致的疾病,其中21羟化酶缺乏症(21-OHD)患者占90％以上,疾病所致的矮身材是该病治疗中较为关注的问题之一。为此我们回顾分析了1978-2001年在我院儿科内分泌专科随访的38例21-OHD患者最终身高(FH),并分析临床分型、性别、诊断早晚、城乡分布、父母身高和服药依从性等因素与靶身高(TH)的关系。     

全国多囊卵巢综合征及其相关疾病专题研讨会纪要

多囊卵巢综合征(PCOS)是妇产科常见的、复杂而难治的内分泌疾病。发生率占育龄妇女的5％-10％,是目前国内外研究的热点和难点。全国多囊卵巢综合征专题研讨会在同道们的共同关心支持下,于2003年12月19日至21日在南京钟山宾馆举行。中华医学会副会长、妇产科学会主任委员、《中华妇产科杂志》名誉总编辑曹泽毅教授、中华医学会韩晓明副秘书长、南京军区南京总医院易学明     

多囊卵巢综合征的现代概念

多囊卵巢综合征PCOS是育龄妇女常见的妇科内分泌疾病,也是病因及发病机制极复杂的疾病。     

急性心肌梗死阴虚病机与短期预后关系及机理探讨

目的 研究急性心肌梗死阴虚病机与短期预后的关系及其神经内分泌机理。方法 按照中医虚证辨证标准将急性心肌梗死患者194例分为典型阴虚组26例、不典型阴虚组61例和非阴虚组107例,分别于清晨卧位采集静脉血测定血浆肾上腺素、去甲肾上腺素、醛固酮、心钠素、皮质醇、心肌酶等,并统计住院天数、住院期间病死率,比较3组间的差异,另选30名健康人为对照组。结果 阴虚组血清肌酸磷酸激酶、肌酸磷酸激酶同功酶、血浆肾上腺素、去甲肾上腺素、醛固酮水平、住院天数、病死率均显著高于非阴虚组(P<0．05或P<0．01);典型与非典型阴虚组之间比较,仅血浆醛固酮与心钠素水平前者显著高于后者(P<0．05,P<0．01);血浆皮质醇水平在阴虚各组之间差异无显著性。结论 急性心肌梗死阴虚患者心肌损害重、住院时间长、病死率高,其机理可能与交感-肾上腺素系统功能亢进,醛固酮活性增强有关。     

环境内分泌干扰物对儿童生长发育与健康的影响

保护儿童免受化学毒物的危害是对现代社会的一大挑战。当今世界 ,儿童有接触近 15 0 0 0种大量生产的化学合成物质的危险[1 ] 。其中许多化学物质广泛分布于环境之中。半数以上的这些化学物质从未进行过潜在毒理试验 ,其对儿童的潜在危险大多不清楚。处于生长发育阶段的儿童 ,环境化学毒物尤其是环境内分泌干扰物肯定对其健康和生长发育产生潜在的不良影响。因此 ,研究环境内分泌干扰物与儿童的健康和生长发育的关系 ,对保护儿童的健康具有重要意义。1.环境内分泌干扰物 :环境内分泌干扰物 (EndocrineDisruptors EndocrineDisruptingChemi…