人源性汉坦病毒噬菌体抗体基因的筛选、测序和表达

目的为了获取人源性ＨＦＲＳ基因工程抗体。方法直接从人体ＰＢＬ构建人全套ＳｃＦｖ、ＶＨ噬菌体表面呈现文库,以ｐａｎｎｉｎｇ方法筛至四级文库,以ＥＬＩＳＡ方法鉴定各克隆抗汉坦病毒活性。结果获得了１４个ＳｃＦｖ阳性克隆和１１个ＶＨ阳性克隆。其中ＳｃＦｖ最高阳性克隆Ａ４１０值为０．４４,ＶＨ最高阳性克隆Ａ４１０值为０．５０。对最高阳性克隆进行了测序分析,证明连接和克隆正确,获得了抗ＨＴＶ人源性ＳｃＦｖ和ＶＨ抗体的基因片段。克隆再转化于ＨＢ２１５１菌株,成功地进行了分泌型抗体片段的表达。结论采用噬菌体抗体库技术直接从人体克隆人源性汉坦病毒噬菌体抗体可行,对ＨＦＲＳ的防治有一定的实际意义     

Altered MR imaging findings in a Japanese female child with PRUNE1-related disorder

Autosomal recessive PRUNE1 mutations on chromosome 1q21.3 are reported to cause a neurodevelopmental disorder with microcephaly, hypotonia, and variable brain malformations. Here, we report a Japanese case with a reported PRUNE1 mutation whose brain magnetic resonance imaging (MRI) showed specific imaging findings that have not been reported before. The patient was a 12-month-old girl, the first child of healthy and nonconsanguineous Japanese parents. She showed global developmental delay, intellectual disability, hypotonia, spastic quadriparesis, and hyperreflexia. Brain MRI showed cerebral and cerebellar atrophy, thin corpus callosum, white matter changes, and abnormal signal intensity of the brainstem, all of which were reported in the literature. In addition, we emphasize the three following imaging findings: a transient cerebral subcortical white matter lesion, atrophy of the midbrain and pontine tegmentum with a preserved pontine base, and abnormal signal intensity of the bilateral swelling putamina and medial portions of the thalami, which emerged after 4 years of age. The whole-exome sequencing (WES) analysis performed at the age of 4 years identified biallelic PRUNE1 variants, namely compound heterozygous mutations (c.[316G > A];[540 T > A],p.[Asp106Asn];[Cys180*]). Although the diagnosis of PRUNE1-related disorder requires WES, we think that these new characteristic MRI findings may help in the diagnosis of PRUNE1-related disorder.     

vHP法在主动脉、冠状动脉及肺动脉一站式成像中的应用研究

目的：探讨320排容积CT可变螺距法（vHP）在急性胸痛患者全主动脉、冠状动脉及肺动脉一站式成像中的临床应用价值。方法：以30例不明原因的急性胸痛患者为实验组,行320排容积CTvHP法扫描：主动脉弓上缘至心底水平采用小螺距心电门控,心底至髂动脉水平采用大螺距不加心电门控。以30例非心电门控大螺距全主动脉320排容积CT扫描患者作为对照组,比较两组的图像质量、辐射剂量以及对比剂使用量。结果：实验组图像对主动脉瓣及主动脉根部的可诊断率（100％,30／30）明显高于对照组（30％,9／30）;所有对照组图像对冠状动脉均无法评价,实验组冠状动脉图像的可诊断率达98％;两组图像对肺动脉的可诊断率差异无统计学意义（P〉0．05）。实验组每例患者的辐射剂量[（1131．73±58．08）mGy·cm]稍高于对照组[（1102．67±70．29）mGy·cm],两组间差异无统计学意义（P〉0．05）;实验组中每例患者的对比剂用量[（96．07±5．62）mL]略高于对照组[（76．00±5．96）mL],差异有统计学意义（P〈0．05）。结论：vHP扫描法可以在不明显增加辐射剂量的前提下一次完成急性胸痛患者主动脉、冠状动脉及肺动脉的扫描,并可减少对比剂的用量,对于进行胸痛患者尽早明确诊断有一定的应用价值。     

Human IgA- and IgM-secreting intestinal plasma cells carry heavily mutated VH region genes

Single IgA- or IgM-secreting plasma cells were isolated from histological sections of human jejunum and terminal ileum, and Ig heavy chain variable (V_H ) region genes were amplified and sequenced. Taken together, 62 of 63 cells analyzed harbored somatically mutated V_H region genes, indicating that the vast majority of both IgA- and IgM-secreting intestinal plasma cells derive from germinal center B cells. On average, rearranged V_H genes of IgA- and IgM-secreting plasma cells showed a mutation frequency of 9.0 % and 8.5 %, respectively, which exceeds the level of somatic mutation of V region genes carried by human memory B cells. Moreover, we detected deletions or insertions in the complementarity-determining regions of 5 of the 58 functional V_H region genes analyzed, suggesting that these alterations may contribute to the diversification of the human antibody repertoire in the course of an immune reaction.     

Variable fragment length allele-specific polymerase chain reaction (VFLASP), a method for simple and reliable genotyping

Single-nucleotide polymorphism (SNP) is a substitution of a single nucleotide at a specific position in the genome. Until now, 585 million SNPs have been identified in the human genome, and therefore, a widely applicable method is desirable to detect a specific SNP. Herein we report a simple and reliable genotyping assay, which seems to be suitable for medium and small size laboratories, as well, to easily genotype most of the SNPs. In our study, all of the possible base variations (A-T, A-G, A-C, T-G, T-C, G-C) were tested to prove the general feasibility of our technique. The basis of the assay is a fluorescent PCR, in which both allele-specific primers, differing only at the 3′ end according to the sequence of the SNP, were present, and the length of one of them was modified with 3 bp by adding an adapter sequence to the 5’ end of that primer. The competitive presence of both allele-specific primers excludes the false amplification of the absent allele (which can happen in simple allele-specific PCR (AS-PCR)) and ensures the amplification of the proper allele(s). Unlike other complicated genotyping methods that use of manipulation of fluorescent dyes for genotyping, we apply an approach based on the length of amplicons from different alleles to differentiate between them. In our experiment (named variable fragment length allele-specific polymerase chain reaction (VFLASP)), the investigated six SNPs, containing the six available base variations, gave clear and reliable results after detecting the amplicons by capillary electrophoresis.     

Automatic Efficiency Optimization of an Axial Compressor with Adjustable Inlet Guide Vanes

The inlet attack angle of rotor blade reasonably can be adjusted with the change of the stagger angle of inlet guide vane (IGV); so the efficiency of each condition will be affected. For the purpose to...     

The analysis of multiple outcomes,multiple variables and variables selection in hematopoietic cell transplantation studies

It is common to study time-to-event data in cancer research such as hematopoietic cell transplantation (HCT) for leukemia. The extensive work has been done for the univariate survival outcome, that is, one event type. However, in practice a subject is often exposed to multiple types of outcomes. In this article, we review various types of right-censored data with multiple outcome types including competing risks data, recurrent event data, and composite endpoints. We also provide hematopoietic cell transplantation data examples.     

用可变扩散湍流模型研究轴对称排气系统红外辐射特性

为研究可变扩散湍流模型在排气系统红外辐射特性计算中的适用性,计算了轴对称亚声速喷管在3~5μm波段的空腔-喷流组合红外辐射特性。喷管流场及温度场采用有限体积法求解N-S方程,湍流模型采用标准k-ε模型和可变扩散模型。红外特性计算采用有限体积法求解吸收-发射性介质条件下的三维辐射传输方程,并考虑大气衰减作用。计算结果与试验对比表明,与标准k-ε模型相比,可变扩散湍流模型能够有效改善亚声速热喷流的核心区长度,从而获得与试验结果一致的红外辐射强度,说明可变扩散湍流模型适用于精确模拟轴对称排气系统的红外辐射特性。 