非小细胞肺癌患者外周血Foxp3+调节性T细胞和Th17的检测与临床意义

目的 通过检测非小细胞肺癌(NSCLC)患者外周血Foxp3+调节性T细胞(TrFoxp3+)、Th17细胞、TrFoxp3+/Th17及相关细胞因子水平,探讨TrFoxp3+、Th17细胞在肺癌发生发展中的相互作用以及是否存在TrFoxp3+/Th17失衡.方法 采用流式细胞术和酶联免疫吸附法(ELISA)分别检测18例健康人、26例NSCLC患者外周血中TrFoxp3+、Th17细胞占CD4+T细胞的比例,以及转化生长因子-β(TGF-β)、IL-17、IL-23的表达水平.结果 NSCLC患者外周血中TrFoxp3+、Th17细胞比例、TrFoxp3+/Th17高于健康对照组(P＜0.05).NSCLC患者外周血TrFoxp3+与Th17细胞成正向直线相关的关系(r=0.81,P＜0.05).不同TNM分期肺癌患者外周血TrFoxp3+/Th17:Ⅳ期肺癌患者高于Ⅰ～Ⅱ期、Ⅲ期肺癌患者(P＜0.05).NSCLC患者血清中TGF-β、IL-17、IL-23水平高于健康对照组,TGF-β在晚期组/健康对照组的比值高于早中期组/健康对照组的比值(P＜0.05).结论 NSCLC患者外周血中TrFoxp3+、Th17细胞比例及TrFoxp3+/Th17较健康人升高,且晚期患者TrFoxp3+/Th17明显升高,提示NSCLC患者可能存在TrFoxp3+/Th17比例失衡,导致肿瘤患者免疫抑制,促进肿瘤发生、发展,可能与二者的细胞因子调节有关.     

失血性休克过程中红细胞携氧-释氧动力学研究

失血性休克过程中,红细胞自身代谢与功能的变化会导致红细胞携氧-释氧能力的改变。本文对不同休克程度红细胞携氧-释氧能力的变化进行了监测,探讨失血性休克的危重程度与红细胞携氧-释氧能力之间的相互关系,并进行生理生化指标分析。本文采用健康SD大鼠20只(雌雄各10只),建立失血性休克模型,分别在初始、失血20%、30%和40%时采集红细胞,并利用血氧分析仪测定红细胞携氧-释氧热力学参数P50,效应时间Tc50、Tr50,同时检测了2,3-DPG、pH值、葡萄糖和乳酸脱氢酶的变化。实验结果表明:随着休克程度的加深,P50值不断增大,乳酸脱氢酶增加,而Tr50、pH值、葡萄糖均呈现减小的趋势,Tc50变化不明显;红细胞携氧能力相对稳定,而向组织释氧的能力仅略有增加,机体氧耗无法得到满足导致多器官衰竭。     

新型超声造影剂对肝纤维化-肝硬化定量诊断的研究

目的:探讨新型超声造影剂对肝纤维化-肝硬化定量诊断的研究.方法:肝纤维化、肝硬化患者各20例,均经肝穿刺烫病理证实,正常对照组10例,无任何肝脏病史,采用实时灰阶超声造影,团注法注入声诺维(SonoVue),测定肝静脉到达时间(HVAT)及肝动脉到达时间(HAAT),两者相减得到肝动静脉渡越时间(HA-V1T).结果:HA-VTT在正常对照组、肝纤维化组、肝硬化组间两两比较均有统计学意义(P<0.05),肝硬化组HA-VTT较正常对照组明显缩短(P<0.01).结论:利用新型超声造影剂测量HA-VTT有望成为定量诊断肝纤维化、肝硬化的新方法.     

糖槭叶抗氧化活性研究

目的：研究糖槭叶的抗氧化活性,为开发新的药用资源提供理论基础和科学依据。方法：通过ESR测定DPPH抑制率、SOD Assay Kit-WST法测定SOD活性、Griess法测定NO的消去作用对糖槭叶多糖体外抗氧化活性进行考察;通过测定小鼠血清中SOD活性和MDA含量对糖槭叶进行体内抗氧化活性研究。结果：测定糖槭叶多糖对DPPH的抑制率为（12.2±6.74）%,SOD活性为（0.27±0.003）U/mg,对NO的消去率为（53.0±2.1）%;空白组、模型组、小剂量组、大剂量组的小鼠血清中SOD活性分别为（16.20±3.00）U/ml、（9.72±0.69）U/ml、（39.70±8.57）U/ml、（22.56±3.08）U/ml,MDA含量分别为（27.43±2.28）nmol/ml、（35.96±5.08）nmol/ml、（15.29±4.02）nmol/ml、（17.70±8.14）nmol/ml。结论：糖槭叶具有显著的抗氧化活性。     

颅脑损伤高压氧治疗时机探讨

目的探讨不同时期高压氧治疗颅脑损伤对预后的影响。方法选取我院应用高压氧治疗的颅脑损伤患者，按照开始治疗时间不同分为两组，其余治疗措施相同，出院时评定预后。结果早期治疗组(ETG)：恢复良好82(74．5％)，轻残14(12．7％)，重残8(7．3％)，死亡6(5．5％)；晚期治疗组(LTG)：恢复良好40(44．9％)，轻残21(23．6％)，重残15(16．9％)，死亡13(14．6％)，两组相比有显著性差异(P＜0．05)。结论ETG疗效较LTG明显提高，提示高压氧治疗颅脑损伤时间越早，预后越好。     

自身免疫性溶血性贫血患者外周血Th1/Th2、Th3/Tr1细胞的检测及意义

目的探讨自身免疫性溶血性贫血(AIHA)患者外周血Th1/Th2、Th3/Tr1细胞状态,分析它们在AIHA发病机制中的作用。方法收集AIHA患者及健康者外周抗凝静脉血,分离纯化淋巴细胞。运用FITC-CD3单抗,Cy5-CD4单抗,PE-CRTH2单抗,以CD3/CD4设门作三色流式细胞术检测Th1/Th2细胞,ELISA法检测血清中Th3细胞相关的细胞因子TGF-β1的含量和Tr1细胞的相关因子IL-10的含量。结果与正常对照相比,AIHA患者外周血CD3+CD4+CRTH2-T细胞(Th1)百分率、CD3+CD4+CRTH2+T细胞(Th2)百分率均下降(P<0.05),而CD4+CRTH2-T/CD4+CRTH2+T比例(Th1/Th2)均明显升高(P<0.01),Th3/Tr1细胞分泌的相关细胞因子TGF-β1和IL-10的含量均降低(P<0.05)。结论AIHA患者外周血存在细胞免疫功能失调,T细胞亚群极化状态发生改变,呈Th1型细胞优势,Th3/Tr1细胞因子含量下降,可能与AIHA的免疫学发病机制有关。     

Asynchronous Bilateral Total Knee Arthroplasty: Predictors of the Functional Outcome and Patient Satisfaction for the Second Knee Replacement

BackgroundThe primary aim of this study is to identify independent preoperative predictors of outcome and patient satisfaction for the second total knee arthroplasty (TKA).MethodsA retrospective cohort of 454 patients undergoing an asynchronous (6 weeks or more apart) bilateral primary TKA were identified from an arthroplasty database. Patient demographics, comorbidities, Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), and Short Form-12 scores were collected preoperatively and 1 year postoperatively. Overall patient satisfaction was assessed at 1 year.ResultsThe 1 year WOMAC pain score (P = .01), and improvement in WOMAC pain (P < .001) and functional (P = .002) scores were significantly lower for the second TKA. Worse preoperative WOMAC pain, function, and stiffness scores were demonstrated to be independent predictors of improvement in the WOMAC pain, function, and stiffness scores, respectively, for both the first and second TKA. The overall rate of satisfaction with the first TKA was 94.0% and 94.7% for the second TKA (P = .67). The rate of satisfaction for the second TKA was 77.8% for patients that were dissatisfied with their first TKA, which was an independent predictor of dissatisfaction (P = .02).ConclusionImprovement in pain and function is less with the second TKA, but the satisfaction rate remains similar. There are common independent predictors for change in the WOMAC score for the first and second TKA; however, the predictors of satisfaction were different with no common factors. Patients that were dissatisfied with their first TKA were more likely to be dissatisfied with their second TKA.Level of Evidence IIPrognostic retrospective cohort study.     

Understanding immune-microbial homeostasis in intestine

Immunologic Research - The mechanisms concerning how the immune system is able to deal with the massive antigen challenge represented by the commensal bacterial flora have been a mystery. Recently...     

Apoptosis in tissue inflammation and allergic disease

Genetic predisposition and environmental instructions tune thresholds for the activation, effector functions and lifespan of T cells, other inflammatory cells and resident tissue cells. Defects in apoptosis and peripheral tolerance in T cells define different allergic phenotypes. In individuals with atopic allergic disease, activated allergen-specific T cells expressing high levels of IFN-gamma predominantly undergo apoptosis in the circulation, skewing the immune response to surviving T helper type 2 (Th2) cells. In affected tissues, these cells switch on effector cytokines and induce the activation and apoptosis of epithelial cells. In individuals with non-atopic monoallergic disease, by contrast, a disturbed balance towards allergen-specific Th2 cells instead of T regulatory type 1 (Tr1) cells characterizes the T cell response.     

CD4+CD25+Tr细胞与非小细胞肺癌相关性研究

目的：探讨CD4^＋CD25^＋Tr细胞与非小细胞肺癌的相关性。方法：采用流式细胞技术分别检测60例非小细胞肺癌患者和20例健康者外周血中CD4^＋CD25^＋Tr细胞数量。结果：非小细胞肺癌患者外周血中CD4^＋CD25^＋Tr细胞表达水平明显高于健康者（P〈0.05）,且与临床分期呈正相关。结论：非小细胞肺癌患者外周血中CD4^＋CD25^＋Tr细胞数量的增多与非小细胞肺癌发病有关。