改进的TTC染色法显示大鼠心肌缺血再灌注损伤

【摘要】目的：比较应用不同2,3,5-氯化三苯基四氮唑(TTC)染色方法对大鼠心肌缺血再灌注损伤后梗死面积的检测效果。方法：将20只SD大鼠(雄性、8周龄,体重250～300g)按随机数字表法分为两组,每组10只,A组：传统TTC染色法组,B组：改进后的TTC染色法组,分别进行大鼠心肌染色,随后计算心肌梗死面积及测定血清cTnI浓度水平。结果：A组和B组均能较好地标记梗死心肌;A组和B组心肌梗死面积百分比无统计学差异（48.69±5.37 % VS 47.41±3.28%,P>0.05）;A组和B组血清cTnI浓度水平无统计学差异（4.51±0.88ng/ml VS 4.70±0.71ng/ml, P>0.05）;但B组心肌切片染色色泽对比度及心肌非梗死区与梗死区区分度均高于A组。结论：改进后的心肌TTC染色法采用在体染色,不仅操作简便,节省了实验时间和经费,而且提高了染色效果,能更准确地反映心肌缺血再灌注损伤的程度。因此改进后的心肌TTC染色法是一种经济、简便、快捷、高效的染色方法。     

色素内镜联合内镜黏膜切除术诊治结直肠侧向发育型肿瘤的临床研究

背景:结直肠侧向发育型肿瘤(LST)与结直肠癌关系密切。目的:探讨色素内镜联合内镜黏膜切除术(EMR)对结直肠LST的临床诊疗价值。方法:纳入2009年7月～2012年2月于达县人民医院行结肠镜检查发现可疑病灶的患者2200例,对病灶内镜下喷洒0.4%靛胭脂行黏膜染色。对染色发现的LST,观察病灶大小并进行形态分型。对LST病灶行EMR或内镜分片黏膜切除术(EPMR),未能行EMR或EPMR者行内镜下活检。结果:黏膜染色后发现结直肠LST患者28例,共30个LST病灶。30个LST病灶中颗粒均一型14个(46.7%),结节混合型8个(26.7%),平坦隆起型5个(16.7%),假凹陷型3个(10.0%);行EMR19个(63.3%),行EPMR6个(20.0%),行内镜下活检5个(16.7%)。病理学检查示早期结直肠癌4个(13.3%),进展期结直肠癌3个(10.0%),腺瘤23个(76.7%)。结论:色素内镜可有效发现结直肠LST病灶。色素内镜联合EMR或EPMR根除LST病灶对早期结直肠癌的防治具有积极意义。     

3种检测方法诊断结核病结果分析

目的比较和评价操作简便、成本低廉、高发现结核患者的不同诊断方法。方法对180例结核门诊就诊患者采用痰涂片、痰培养、血清结核抗体（TB-Ab）3种方法进行检测，并与正常体查人群对照。结果180例结核门诊就诊患者痰涂片阳性率为38．34％（69/180），痰培养阳性率为40．00％（72/180），血清结核抗体阳性率为42．23％（76/180）。对照组50例痰涂片、痰培养均阴性，血清结核抗体阳性率为4．00％（2/50）。结论3种检测方法差异不大（P〉0．05），为提高结核患者确诊率，建议对可疑肺结核患者采用3种方法联合检测。     

快速染色的妇科肿瘤细胞核的结构观察与分析

本文用细胞快速染液对妇科常见肿瘤的细胞核结构进行了观察。结果表明，细胞快速染液能清晰地显示癌细胞核和核仁的形态结构及数目。根据大量的观察结果作者提出：核仁的数目和结构形态是推测细胞“良”“恶”的窗口，是确定癌细胞的肯定指标之一。对癌细胞的核膜增厚和染色质网状结构提出了讨论。     

A histochemical method for the demonstration of calcifying cartilage

Summary The demonstration of histochemical characteristics in calcifying cartilage is fraught with methodological difficulties including the distinction of mineralized from unmineralized cartilage and the demonstration of cell detail in relatively hard tissue. This study uses the decalcified bone matrix-induced enchondral (endochondral) ossification system to demonstrate a technique of methylmethacrylate embedding, thin sections, and a combination of histochemical stains that distinguishes mineralized from unmineralized cartilage while preserving excellent cell detail. These techniques are applicable to other areas of enchondral ossification and are exemplified by the staining of growth plates.     

精子尾部低渗膨胀试验的相关因子及染色法

共检测110例男性不育患者的精液标本,确证精子尾部低渗膨胀(HOS)试验与精子活动率有较显著的正相关(r=0.273,n=102,P<0.01);与精子膜表面麦芽凝集素(WGA)受体亦成正相关(r=0.251,n=72,P<0.05);但与精子膜表面附着的抗精抗体免疫微珠(IB)试验无关(r=0.027,n=62,P>0.05);与精液有无支原体感染无关(t=0.543,df=32,P>0.05);精液中圆细胞数量对 HOS 试验无影响(F=0.413,方差自由度为f_1=3,f_2=100,P>0.05)。故认为以上试验互不能取代,只能互相参考。我们用简便而可靠的铁苏木精染色法改进了 HOS 试验技术,以便于这种确有实用价值的精子功能检测技术在临床上推广。     

Silver staining of fibrous neuroglia by means of physical development

Summary A simple and reproducible technique for demonstrating fibrous neuroglia has been worked out on the basis of a new silver staining principle. It may be applied to formol-fixed material even after several years. The principle of it is as follows: In an acidic solution uranyl ions combine with or are adsorbed on fibrous neuroglia. The bound uranyl ions are replaced by colloidal silver with the help of chemical reactions. The silver grains are increased in size up to microscopic dimensions by a special physical developer.

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Zusammenfassung Eine einfache und reproduzierbare Methode wurde für die Darstellung der Faserglia auf Grund eines neuen Silberfärbungprinzips ausgearbeitet. Das Verfahren kann selbst nach mehrjähriger Formalinfixierung an Gefrierschnitten auch nach Gelatineeinbettung mit Erfolg ausgeführt werden.Das Grundprinzip ist folgendes: uranylionen adsorbieren oder binden sich in saurer Lösung an die Faserglia. Für die gebundenen Uranylionen wird in chemischen Reaktionen colloidales Silber substituiert. Die Silberkeime werden mit Hilfe eines physikalischen Entwicklers bis zu mikroskopischen Dimensionen vergrößert.

     

微孢子虫检测技术的研究进展

微孢子虫是专性细胞内寄生的真核生物,其作为人类新发病原越来越受到重视,故快速、准确的检测技术对于微孢子虫病的防治显得尤为重要。本文简要综述了以染色技术、免疫组化技术和分子生物学技术等为主的微孢子虫识别方法的研究进展,以期为有效防治微孢子虫病提供参考。     

Comparison of interfacial characteristics of adhesive bonding to superficial versus deep dentine using SEM and staining techniques

OBJECTIVES: A primary reason for premature failure of Class II composite restorations is recurrent decay at the gingival margin. The integrity of the gingival margin depends on the bond formed with dentine. Using scanning electron microscopy (SEM) and histomorphologic technique, the purpose of this study was to characterise the adhesive/dentine (a/d) bond in dentine sections from the dentinoenamel junction (DEJ) and the cementoenamel junction (CEJ) with commercial adhesives of varying hydrophobic/hydrophilic composition. MATERIALS AND METHODS: The occlusal 1/3 of the crown was removed from 20 human third molars, this exposed the surface used for the DEJ sections. The teeth were sectioned occluso-gingivally into equal halves: one-half representing the DEJ; the remaining half was sectioned 4mm deep to the exposed surface (CEJ). Each half was treated with the same adhesive. The teeth were randomly selected for treatment with Single Bond (SB, 3M) or Dentastic UNO (UNO, Pulpdent) using wet bonding technique as per manufacturers' instructions. Thin (3-5mum) sections of native a/d interfaces were cut and stained with Goldner's trichrome for light microscopic (LM) examination. Companion slabs were polished and acid-bleach treated, then analysed with SEM. RESULTS: DEJ sections: SB/dentine specimens had approximately 4mum exposed protein at the interface, UNO infiltrated the depth of the demineralised dentine and encapsulated exposed protein. CEJ sections: SB/dentine specimens had highly variable interface structure with localized exposed protein, UNO/dentine exhibited 3-5mum exposed protein at the interface. The quality of the interface was exaggerated and the above differences were not revealed based on SEM characterisation. CONCLUSIONS: As a result of adhesive phase separation, SB does not form structurally integrated a/d bonds at the DEJ or CEJ. The increased hydrophilic composition of UNO contributes to the formation of an integrated a/d bond at the DEJ, but structural differences and increased moisture leads to unprotected protein at the a/d interface in the CEJ sections. The inability of the SEM technique to reveal the complexity of the interface could be related to the modifications of the a/d interface caused by polishing and acid-bleach treatment.     

大鼠大脑皮层星形胶质细胞的传代培养

目的：获得纯度较高的星形胶质细胞材料用于进一步实验。方法：采用大鼠神经胶质细胞的原代培养和传代培养方法。取生后24h以内Wistar大鼠脑皮层，用机械法和胰蛋白酶消化法分散细胞，制成细胞悬液，接种于无底物黏附培养瓶中，置37℃，5％CO2培养箱中培养。培养液为DMEM/F12混合培养液。待细胞铺满瓶底后传代，并对传代细胞进行形态观察、吖啶橙荧光染色和星形胶质细胞的免疫细胞化学鉴定。结果：通过传代得到了形态典型含量较高的星形胶质细胞。结论：在无底物黏附培养的条件下，星形胶质细胞在体外能够得到良好的生长，并被纯化。