106例新生儿缺氧缺血性脑病血清酶学变化分析

目的探讨新生儿缺氧缺血性脑病(HIE)的发生、程度和预后与血清酶学变化的临床相关性。方法回顾中、重度HIE患儿106例血清酶学检测的结果。结果所有患儿均有血清酶活性不同程度升高,中、重度各酶值比较差异有统计学意义(P<0.05)。结论血清酶学变化测定对新生儿缺血缺氧性脑病早期诊断及预后有指导意义,可降低后遗症发生率和患儿病死率。     

血清前白蛋白检测在肝脏疾病诊断中的应用

目的探讨血清前白蛋白(PA)检测对肝脏疾病诊断的价值。方法对200例各种肝脏疾病患者(急性肝炎组50例、慢性肝炎组50例、肝硬化组50例、肝癌组50例)和60例健康体检者(对照组)血清前白蛋白的水平进行测定,采用方差分析比较各组前白蛋白的差异。结果健康体检者、急性肝炎组、慢性肝炎组、肝硬化组前白蛋白逐渐降低。结论前白蛋白检测对各种肝脏疾病的诊断和病情具有重要的临床价值。     

Evaluation of the Roche Elecsys Toxo IgG and IgM electrochemiluminescence immunoassay for the detection of gestational Toxoplasma infection

Unidentified gestational infection with Toxoplasma gondii may lead to fetal infection with severe complications later in childhood. Because diagnosis of maternal infection solely depends on serology, routine tests with high sensitivity and specificity are required. In this study, the new Roche Elecsys Toxo IgG and IgM immunoassay was compared with Sabin-Feldman dye test and immunosorbent agglutination assay-IgM as reference test. Serum samples were analyzed from 927 pregnant women, including 100 negative, 706 chronic, and 121 acute infections. The combination of both Elecsys IgG and IgM assays demonstrated high sensitivity and specificity of 97.1% and 100.0%, respectively, and a positive and negative predictive value of 100.0% and 81.3%, respectively. The Elecsys assay is a useful tool as a first-line screening method to detect gestational infections. However, if gestational infection is assumed, confirmatory testing by a reference laboratory might be necessary to discriminate between pre- and postconceptional infection to start antiparasitic treatment to avoid mother-to-fetus transmission and severe sequelae.     

Complementary exams in the diagnosis of american tegumentary leishmaniasis

The diagnosis of American Tegumentary Leishmaniasis is a difficult but essential task when considering the high toxicity profile of the drugs available. Since the discovery of its etiologic agent, numerous diagnostic tests have been developed. None of the tests available today can be considered as the gold standard, since they do not add enough accuracy for the disease detection. Good epidemiological and clinical knowledge of the disease are fundamental precepts of the dermatology practice and precede the rational use of existing diagnostic tests. In this article we aim, through extensive literature review, to recall fundamental concepts of any diagnostic test. Subsequently, based on this information, we will weave important comments about the characteristics of existing diagnostic tests, including immunological tests such as Montenegro''s skin test, serology and detection of parasites by direct examination, culture or histopathology. Finally we will discuss the new technologies and options for the diagnosis of Cutaneous Leishmaniasis. The molecular biology technique is considered a promising tool, promoting the rapid identification of the species involved. We also aim to educate dermatologists about a disease with high morbidity and assist in its difficult recognition.     

血清药理学方法研究蛇葡萄素抗B16黑色素瘤的作用

目的：研究蛇葡萄素对黑色素瘤的抑制作用。方法：以血清药理学方法考察不同剂量的蛇葡萄素含药血清对B16黑色素瘤细胞的抑制作用，分别用MTT法及流式细胞术进行评价。结果：0．420mmol／kg、0．560mmol／kg剂量组的C57BL／6小鼠含药血清能有效抑制黑色素瘤B16细胞，与空白血清中加入浓度相当的蛇葡萄素的抑制率相同；两个剂量组的含药血清使肿瘤细胞的分裂增殖指数分别降低了19．1％、21．7％。结论：蛇葡萄素在血药浓度达峰期对小鼠黑色素瘤B16细胞的抑制可能以原型物形式起作用，抑制细胞的DNA合成。     

Results of the 2nd part Scientific Workshop of the ECCO (II): Measures and markers of prediction to achieve, detect, and monitor intestinal healing in Inflammatory Bowel Disease

The healing of the intestine is becoming an important objective in the management of inflammatory bowel diseases. It is associated with improved disease outcome. Therefore the assessment of this healing both in clinical studies and routine practice is a key issue. Endoscopy for the colon and terminal ileum and computerized tomography or magnetic resonance imaging for the small bowel are the most direct ways to evaluate intestinal healing. However, there are many unsolved questions about the definition and the precise assessment of intestinal healing using these endoscopic and imaging techniques. Furthermore, these are relatively invasive and expensive procedures that may be inadequate for regular patients' monitoring. Therefore, biomarkers such as C-reactive protein and fecal calprotectin have been proposed as surrogate markers for intestinal healing. Nevertheless, the sensitivity and specificity of these markers for the prediction of healing may be insufficient for routine practice. New stool, blood or intestinal biomarkers are currently studied and may improve our ability to monitor intestinal healing in the future.     

代谢组学在卵巢癌中的应用

代谢组学是近年发展起来的对某一生物或细胞所有低分子代谢产物进行定性和定量分析的新学科.代谢组学是继基因组学、转录组学及蛋白质组学之后生命科学的重要组成之一.卵巢癌的发生发展必然伴随着代谢改变.代谢组学能给卵巢癌代谢水平一个完整的信息,并能给卵巢癌早期诊断及个体化治疗带来广阔前景.