顺丁烯二酸酐改性环氧二甲基丙烯酸酯的合成与应用

为了合成顺丁烯二酸酐改性的环氧二甲基丙烯酸酯树脂及其物理机械性能。按量取E51环氧树脂、甲基丙烯酸、顺丁烯二酸酐，与催化剂N，N-二甲基苄胺一起进行反应。检测了由合成EAM单体或EMA单体配制复合树脂的压缩强度、径向拉伸强度、表面硬度、固化深度、吸水值和水溶解值。与EMA单体比较，含EAM单体的复合树脂明显增强了压缩强度、径向拉伸强度、表面硬度、固化深度值，降低了吸水值和水溶解值。     

农村公共卫生投融资机制研究样本抽取的区域分类方法

目的：构建农村公共卫生投融资机制抽样研究的区域分类指标体系,并计算分类标志值.方法：本文在广泛收集我省农村公共卫生投融资方面的资料数据的基础上,运用专家咨询法确立符合本研究领域特征的指标体系和指标权重,并运用极差法对实际数据进行无量纲化处理,采用加权平均法计算出我省14个地州市的区域分类标志值.结果：我省14个地（州、市）的分类标志值虽然极差较大（反映发展不平衡）,但主要分布于（40,70）这一区间内,其中,大于60的有5个地（州、市）,小于50的有5个,在50-60之间的有4个,我们依此将其分为三个类别的区域.结论：经过专家咨询所筛选的9个指标所计算的标志值能够较好地反映一个地区的农村公共卫生投融资的现状,可以作为本研究抽样的依据.     

氨氯地平复方丹参滴丸对高血压大鼠血管壁重塑的干预

目的：探讨氨氯地平片（络活喜）加复方丹参滴丸对自发性高血压大鼠（SHR）血管壁重塑的干预。方法：将32只10周龄雄性SHR随机分成空白对照组（SHRc）、阴性对照组（SHRNs）、阳性对照组（SHRA）和治疗组（SHRA＋D）四组，每组8只。空白对照组未作治疗即测定各指标，并与8只同周龄Wistar鼠比较。除空白对照组外，后三组分别经生理盐水、络活喜、络活喜加复方丹参滴丸治疗8周后处死，测定各项指标。结果：与wistar组比较，空白对照组及阴性对照组血压明显增高、管腔增大、血管壁明显增厚，尤以血管中层平滑肌细胞（VSMC）肥大为主；经治疗后，阳性对照组及治疗组血压明显降低，血管壁增厚得到遏制；阳性对照组与治疗组之间比较，治疗组血管壁变薄更明显，但无统计学意义。结论：络活喜通过降低血压遏制SHR血管壁重塑，加复方丹参滴丸治疗能进一步遏制血管壁重塑，但可能表现为时效关系。     

中心组合设计优化法筛选独一味分散片制备工艺

 目的　中心组合设计法优化独一味分散片制备处方。方法　采用粉末直接压片,以分散片崩解时间、木犀草苷溶出的t₈₀及分散片混悬后的混悬系数为考察指标,应用中心多点等距设计法及效应面优化法对独一味分散片处方进行筛选,通过对自变量各水平的多元线性回归及二项式拟合,选取较佳工艺,并进行预测分析。结果　考察指标与各因素间具有较好的相关性,优化后独一味分散片处方为12％PVPP为崩解剂、14％预胶化淀粉为溶胀剂、3％微粉硅胶为润滑剂和助流剂、45％MCC为填充剂。优化条件下制备的分散片崩解时间为20.6s,木犀草苷溶出的t₈₀为1.68 min,分散片混悬后的混悬系数为0.068 9。结论 中心组合设计法具有应用简便、预测性好等优点,筛选的处方可以在最适宜的范围内制备一定硬度且具有可被接受口味的速释分散片。     

Biocompatibility evaluation of dicalcium phosphate/calcium sulfate/poly (amino acid) composite for orthopedic tissue engineering in vitro and in vivo

In vitro cytocompatibility of ternary biocomposite of dicalcium phosphate (DCP) and calcium sulfate (CS) containing 40 wt% poly (amino acid) (PAA) was evaluated using L929 ?broblasts and MG-63 osteoblast-like cells. Thereafter, the biocompatibility of biocomposite in vivo was investigated using an implantation in muscle and bone model. In vitro L929 and MG-63 cell culture experiments showed that the composite and PAA polymer were noncytotoxic and allowed cells to adhere and proliferate. The scanning electron microscope (SEM) confirmed that two kinds of cells maintained their phenotype on all of samples surfaces. Moreover, the DCP/CS/PAA composite showed higher cellular viability than that of PAA; meanwhile, the cell proliferation and ALP activity were much higher when DCP/CS had added into PAA. After implanted in muscle of rabbits for 12 weeks, the histological evaluation indicated that the composite exhibited excellent biocompatibility and no inflammatory responses were found. When implanted into bone defects of femoral condyle of rabbits, the composite was combined directly with the host bone tissue without fibrous capsule tissue, which shown good biocompatibility and osteoconductivity. Thus, this novel composite may have potential application in the clinical setting.     

Balancing mechanical strength with bioactivity in chitosan–calcium phosphate 3D microsphere scaffolds for bone tissue engineering: air- vs. freeze-drying processes

The objective of this study was to evaluate the potential benefit of 3D composite scaffolds composed of chitosan and calcium phosphate for bone tissue engineering. Additionally, incorporation of mechanically weak lyophilized microspheres within those air-dried (AD) was considered for enhanced bioactivity. AD microsphere, alone, and air- and freeze-dried microsphere (FDAD) 3D scaffolds were evaluated in vitro using a 28-day osteogenic culture model with the Saos-2 cell line. Mechanical testing, quantitative microscopy, and lysozyme-driven enzymatic degradation of the scaffolds were also studied. FDAD scaffold showed a higher concentration (p?<?0.01) in cells per scaffold mass vs. AD constructs. Collagen was ～31% greater (p?<?0.01) on FDAD compared to AD scaffolds not evident in microscopy of microsphere surfaces. Alternatively, AD scaffolds demonstrated a superior threefold increase in compressive strength over FDAD (12 vs. 4?MPa) with minimal degradation. Inclusion of FD spheres within the FDAD scaffolds allowed increased cellular activity through improved seeding, proliferation, and extracellular matrix production (as collagen), although mechanical strength was sacrificed through introduction of the less stiff, porous FD spheres.     

Experimental and Numerical Study on the Mechanical Behavior of Composite Steel Structure under Explosion Load

Most engineering structures are composed of basic components such as plates, shells, and beams, and their dynamic characteristics under explosion load determine the impact resistance of the structure. In this paper, a three-dimensional composite steel structure was designed using a beam, plate, and other basic elements to study its mechanical behavior under explosion load. Subsequently, experiments on the composite steel structure under explosion load were carried out to study its mechanical behavior, and the failure mode and deformation data of the composite steel structure were obtained, which provided important experimental data regarding the dynamic response and mechanical behavior of the composite steel structure under explosion load. Then, we independently developed a parallel program with the coupled calculation method to solve the numerical simulation of the dynamic response and failure process of the composite steel structure under explosion load. This program adopts the Euler method as a whole, and Lagrange particles are used for materials that need to be accurately tracked. The numerical calculation results are in good agreement with the experimental data, indicating that the developed parallel program can effectively deal with the large deformation problems of multi-medium materials and the numerical simulation of the complex engineering structure failures subjected to the strong impact load.     

Mediterranean Diet and Cardiovascular Disease: A Critical Evaluation of A Priori Dietary Indexes

The aim of this paper is to analyze the a priori dietary indexes used in the studies that have evaluated the role of the Mediterranean Diet in influencing the risk of developing cardiovascular disease. All the studies show that this dietary pattern protects against cardiovascular disease, but studies show quite different effects on specific conditions such as coronary heart disease or cerebrovascular disease. A priori dietary indexes used to measure dietary exposure imply quantitative and/or qualitative divergences from the traditional Mediterranean Diet of the early 1960s, and, therefore, it is very difficult to compare the results of different studies. Based on real cultural heritage and traditions, we believe that the a priori indexes used to evaluate adherence to the Mediterranean Diet should consider classifying whole grains and refined grains, olive oil and monounsaturated fats, and wine and alcohol differently.